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Literature summary for 3.5.1.18 extracted from

  • Uda, N.R.; Creus, M.
    Selectivity of inhibition of N-succinyl-L,L-diaminopimelic acid desuccinylase in bacteria: the product of dapE-gene is not the target of L-captopril antimicrobial activity (2011), Bioinorg. Chem. Appl., 2011, 306465.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli XL1-Blue cells and in Salmonella enterica strain TN5911 Salmonella enterica

Inhibitors

Inhibitors Comment Organism Structure
L-captopril low-micromolar inhibitor, DapE is not the main target of L-captopril inhibition Salmonella enterica

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ the enzyme possesses a catalytic domain with a di-zinc active site Salmonella enterica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41600
-
2 * 41600, estimated from amino acid sequence Salmonella enterica
42000
-
2 * 42000, SD-PAGE Salmonella enterica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-succinyl-LL-2,6-diaminoheptanedioate + H2O Salmonella enterica i.e. N-succinyl-L,L-diaminopimelic acid succinate + LL-2,6-diaminoheptanedioate
-
?

Organism

Organism UniProt Comment Textmining
Salmonella enterica
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-succinyl-LL-2,6-diaminoheptanedioate + H2O i.e. N-succinyl-L,L-diaminopimelic acid Salmonella enterica succinate + LL-2,6-diaminoheptanedioate
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 41600, estimated from amino acid sequence Salmonella enterica
homodimer 2 * 42000, SD-PAGE Salmonella enterica

Synonyms

Synonyms Comment Organism
DapE
-
Salmonella enterica
N-succinyl-L,L-diaminopimelic acid desuccinylase
-
Salmonella enterica

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0018
-
L-captopril pH and temperature not specified in the publication Salmonella enterica

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0033
-
pH and temperature not specified in the publication Salmonella enterica L-captopril