Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli XL1-Blue cells and in Salmonella enterica strain TN5911 | Salmonella enterica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-captopril | low-micromolar inhibitor, DapE is not the main target of L-captopril inhibition | Salmonella enterica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | the enzyme possesses a catalytic domain with a di-zinc active site | Salmonella enterica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41600 | - |
2 * 41600, estimated from amino acid sequence | Salmonella enterica |
42000 | - |
2 * 42000, SD-PAGE | Salmonella enterica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-succinyl-LL-2,6-diaminoheptanedioate + H2O | Salmonella enterica | i.e. N-succinyl-L,L-diaminopimelic acid | succinate + LL-2,6-diaminoheptanedioate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-succinyl-LL-2,6-diaminoheptanedioate + H2O | i.e. N-succinyl-L,L-diaminopimelic acid | Salmonella enterica | succinate + LL-2,6-diaminoheptanedioate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 41600, estimated from amino acid sequence | Salmonella enterica |
homodimer | 2 * 42000, SD-PAGE | Salmonella enterica |
Synonyms | Comment | Organism |
---|---|---|
DapE | - |
Salmonella enterica |
N-succinyl-L,L-diaminopimelic acid desuccinylase | - |
Salmonella enterica |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0018 | - |
L-captopril | pH and temperature not specified in the publication | Salmonella enterica |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0033 | - |
pH and temperature not specified in the publication | Salmonella enterica | L-captopril |