Application | Comment | Organism |
---|---|---|
drug development | development of antimicrobial agents that target DapE | Haemophilus influenzae |
Crystallization (Comment) | Organism |
---|---|
ultrapure recombinant DapE with one and two zinc ions bound in the active site, respectively, at 16°C, by vapor diffusion in hanging drops containing 1 ml of precipitant solution containing 1 M ammonium sulfate, 0.2 M NaCl, and 0.1 M Na acetate, pH 4.4, and 0.001 ml of 13 mg/ml of DapE with three equivalents of zinc, 2 weeks, X-ray diffraction structure determination and analysis at 2.0-2.3 A resolution | Haemophilus influenzae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | design of structure-based, catalytic inhibitors, overview | Haemophilus influenzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
730 | - |
N-succinyl LL-diaminopimelic acid | pH not specified in the publication, temperature not specified in the publication | Haemophilus influenzae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
sulfate | in one of the monomers of the ZnZn_DapE structure, both of these residues form a charged dipole interaction with a sulfate ion, a possible mimic of the carboxylic group of the substrate | Haemophilus influenzae | |
Zn2+ | required for activity, DapE has one or two zinc ions bound in the active site, the two forms show different activity, structures of monometalated and dimetalated forms, overview | Haemophilus influenzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41500 | - |
2 * 41500, SDS-PAGE | Haemophilus influenzae |
83000 | - |
about | Haemophilus influenzae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-succinyl LL-diaminopimelic acid + H2O | Haemophilus influenzae | - |
succinate + LL-2,6-diaminoheptanedioate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemophilus influenzae | P44514 | gene dapE | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
N-succinyl-LL-2,6-diaminoheptanedioate + H2O = succinate + LL-2,6-diaminoheptanedioate | structure-activity relationship and catalytic mechanism of peptide bond cleavage by DapE enzymes, overview. The catalytic domain is composed of residues 1-179 and 293-376 | Haemophilus influenzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-succinyl LL-diaminopimelic acid + H2O | - |
Haemophilus influenzae | succinate + LL-2,6-diaminoheptanedioate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 41500, SDS-PAGE | Haemophilus influenzae |
More | the core of the catalytic domain consists of an eight-stranded twisted beta-sheet that is sandwiched between seven alpha-helices, active site structure and structure-activity relationship, overview | Haemophilus influenzae |
Synonyms | Comment | Organism |
---|---|---|
DapE | - |
Haemophilus influenzae |
N-succinyl-L,L-diaminopimelic acid desuccinylase | - |
Haemophilus influenzae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
140 | - |
N-succinyl LL-diaminopimelic acid | pH not specified in the publication, temperature not specified in the publication | Haemophilus influenzae |
General Information | Comment | Organism |
---|---|---|
metabolism | DapE is involved in the meso-diaminopimelate, mDAP/lysine biosynthetic pathway | Haemophilus influenzae |
physiological function | DapE is essential for cell growth and proliferation | Haemophilus influenzae |