Literature summary for 3.5.1.16 extracted from

Hernandez, V.M.; Girard, L.; Hernandez-Lucas, I.; Vazquez, A.; Ortiz-Ortiz, C.; Diaz, R.; Dunn, M.F.
Genetic and biochemical characterization of arginine biosynthesis in Sinorhizobium meliloti 1021 (2015), Microbiology, 161, 1671-1682 .

Search for more literature for 3.5.1.16

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli K-12. Because the purified 6His-Sumo-ArgE loses all activity following removal of the affinity tag, it is not possible to characterize the tag-free enzyme	Sinorhizobium meliloti

Inhibitors

Inhibitors	Comment	Organism	Structure
arginine	-	Sinorhizobium meliloti

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4	-	N2-Acetyl-L-ornithine	pH 8.0, 30°C	Sinorhizobium meliloti

Metals/Ions

Metals/Ions	Comment	Organism	Structure
CoCl2	metal ion removal from the purified Sumo-ArgE decreases enzyme activity by nearly 90% in reactions without added CoCl2 in comparison with those containing 0.2 mM CoCl2	Sinorhizobium meliloti
MnCl2	metal ion removal from the purified Sumo-ArgE decreases enzyme activity by nearly 90% in reactions without added CoCl2 in comparison with those containing 0.2 mM CoCl2. MnCl2 (0.2 mM) added to the reaction in place of CoCl2 restores activity to about 40 % of that seen with CoCl2	Sinorhizobium meliloti

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
N2-acetyl-L-ornithine + H2O	Sinorhizobium meliloti	-	acetate + L-ornithine	-	?

Organism

Organism	UniProt	Comment	Textmining
Sinorhizobium meliloti	Q92Y75	-	-

Purification (Commentary)

Purification (Comment)	Organism
because the purified 6His-Sumo-ArgE loses all activity following removal of the affinity tag, it is not possible to characterize the tag-free enzyme	Sinorhizobium meliloti

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N2-acetyl-L-ornithine + H2O	-	Sinorhizobium meliloti	acetate + L-ornithine	-	?

Synonyms

Synonyms	Comment	Organism
ArgE	-	Sinorhizobium meliloti
N-acetylornithine deacetylase	-	Sinorhizobium meliloti
NAO deacetylase	-	Sinorhizobium meliloti

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Sinorhizobium meliloti

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Sinorhizobium meliloti

General Information

General Information	Comment	Organism
malfunction	inactivation of argE gene does not cause arginine auxotrophy or marked growth defects	Sinorhizobium meliloti

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)