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Literature summary for 3.5.1.16 extracted from

  • Vogel, H.J.; Bonner, D.M.
    Acetylornithinase of Escherichia coli: Partial purification and some properties (1956), J. Biol. Chem., 218, 97-106.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
glutathione
-
Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Escherichia coli
EDTA
-
Escherichia coli
Ni2+
-
Escherichia coli
p-chloromercuribenzoate
-
Escherichia coli
Zn2+
-
Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ enzyme is a metalloprotein, activated by a Co2+ complex Escherichia coli
Co2+ activity increases 8fold Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N2-acety-L-ornithine + H2O Escherichia coli step in arginine biosynthesis L-ornithine + acetate
-
?
N2-acety-L-ornithine + H2O Erwinia sp. step in arginine biosynthesis L-ornithine + acetate
-
?
N2-acety-L-ornithine + H2O Klebsiella sp. step in arginine biosynthesis L-ornithine + acetate
-
?
N2-acety-L-ornithine + H2O Proteus sp. step in arginine biosynthesis L-ornithine + acetate
-
?
N2-acety-L-ornithine + H2O Salmonella sp. step in arginine biosynthesis L-ornithine + acetate
-
?
N2-acety-L-ornithine + H2O Serratia sp. step in arginine biosynthesis L-ornithine + acetate
-
?
N2-acety-L-ornithine + H2O Shigella sp. step in arginine biosynthesis L-ornithine + acetate
-
?
N2-acety-L-ornithine + H2O Aerobacter sp. step in arginine biosynthesis L-ornithine + acetate
-
?
N2-acety-L-ornithine + H2O Escherichia coli B / ATCC 11303 step in arginine biosynthesis L-ornithine + acetate
-
?
N2-acety-L-ornithine + H2O Escherichia coli 160-37 step in arginine biosynthesis L-ornithine + acetate
-
?
N2-acety-L-ornithine + H2O Escherichia coli ATCC 9637 step in arginine biosynthesis L-ornithine + acetate
-
?

Organism

Organism UniProt Comment Textmining
Aerobacter sp.
-
-
-
Erwinia sp.
-
-
-
Escherichia coli
-
K-12
-
Escherichia coli
-
B
-
Escherichia coli
-
160-37
-
Escherichia coli
-
ATCC9637
-
Escherichia coli 160-37
-
160-37
-
Escherichia coli ATCC 9637
-
ATCC9637
-
Escherichia coli B / ATCC 11303
-
B
-
Klebsiella sp.
-
-
-
Proteus sp.
-
-
-
Salmonella sp.
-
-
-
Serratia sp.
-
-
-
Shigella sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
800
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-DL-methionine + H2O
-
Escherichia coli methionine + acetate
-
?
N-acetyl-DL-methionine + H2O
-
Erwinia sp. methionine + acetate
-
?
N-acetyl-DL-methionine + H2O
-
Klebsiella sp. methionine + acetate
-
?
N-acetyl-DL-methionine + H2O
-
Proteus sp. methionine + acetate
-
?
N-acetyl-DL-methionine + H2O
-
Salmonella sp. methionine + acetate
-
?
N-acetyl-DL-methionine + H2O
-
Serratia sp. methionine + acetate
-
?
N-acetyl-DL-methionine + H2O
-
Shigella sp. methionine + acetate
-
?
N-acetyl-DL-methionine + H2O
-
Aerobacter sp. methionine + acetate
-
?
N-acetyl-DL-methionine + H2O
-
Escherichia coli B / ATCC 11303 methionine + acetate
-
?
N-acetyl-DL-methionine + H2O
-
Escherichia coli 160-37 methionine + acetate
-
?
N-acetyl-DL-methionine + H2O
-
Escherichia coli ATCC 9637 methionine + acetate
-
?
N2-acety-L-ornithine + H2O step in arginine biosynthesis Escherichia coli L-ornithine + acetate
-
?
N2-acety-L-ornithine + H2O step in arginine biosynthesis Erwinia sp. L-ornithine + acetate
-
?
N2-acety-L-ornithine + H2O step in arginine biosynthesis Klebsiella sp. L-ornithine + acetate
-
?
N2-acety-L-ornithine + H2O step in arginine biosynthesis Proteus sp. L-ornithine + acetate
-
?
N2-acety-L-ornithine + H2O step in arginine biosynthesis Salmonella sp. L-ornithine + acetate
-
?
N2-acety-L-ornithine + H2O step in arginine biosynthesis Serratia sp. L-ornithine + acetate
-
?
N2-acety-L-ornithine + H2O step in arginine biosynthesis Shigella sp. L-ornithine + acetate
-
?
N2-acety-L-ornithine + H2O step in arginine biosynthesis Aerobacter sp. L-ornithine + acetate
-
?
N2-acety-L-ornithine + H2O step in arginine biosynthesis Escherichia coli B / ATCC 11303 L-ornithine + acetate
-
?
N2-acety-L-ornithine + H2O step in arginine biosynthesis Escherichia coli 160-37 L-ornithine + acetate
-
?
N2-acety-L-ornithine + H2O step in arginine biosynthesis Escherichia coli ATCC 9637 L-ornithine + acetate
-
?
Nalpha-acetyl-L-ornithine + H2O
-
Escherichia coli L-ornithine + acetate
-
?
Nalpha-acetyl-L-ornithine + H2O
-
Erwinia sp. L-ornithine + acetate
-
?
Nalpha-acetyl-L-ornithine + H2O
-
Klebsiella sp. L-ornithine + acetate
-
?
Nalpha-acetyl-L-ornithine + H2O
-
Proteus sp. L-ornithine + acetate
-
?
Nalpha-acetyl-L-ornithine + H2O
-
Salmonella sp. L-ornithine + acetate
-
?
Nalpha-acetyl-L-ornithine + H2O
-
Serratia sp. L-ornithine + acetate
-
?
Nalpha-acetyl-L-ornithine + H2O
-
Shigella sp. L-ornithine + acetate
-
?
Nalpha-acetyl-L-ornithine + H2O
-
Aerobacter sp. L-ornithine + acetate
-
?
Nalpha-acetyl-L-ornithine + H2O
-
Escherichia coli B / ATCC 11303 L-ornithine + acetate
-
?
Nalpha-acetyl-L-ornithine + H2O
-
Escherichia coli 160-37 L-ornithine + acetate
-
?
Nalpha-acetyl-L-ornithine + H2O
-
Escherichia coli ATCC 9637 L-ornithine + acetate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6 8
-
Escherichia coli