Activating Compound | Comment | Organism | Structure |
---|---|---|---|
mono-benzyl malonate | 25 mM, activation to 109% of control | Thermococcus litoralis |
Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli | Thermococcus litoralis |
overexpressed in Escherichia coli, modification of N-terminal residues of the recombinant enzyme to enhance expression in Escherichia coli using the vector pUCcer11. The recombinant enzyme has an insertion of four amino acids (Glu-Pro-Leu-Phe) between the N-terminal methionine and the second residue lysine of the wild type | Thermococcus litoralis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 1 mM, 54% inhibition | Thermococcus litoralis | |
4-hydroxymercuribenzoate | 1 mM, 67% inhibition | Thermococcus litoralis | |
Ba2+ | 0.01 mM, 17% inhibition | Thermococcus litoralis | |
benzamidine | 0.1 mM, 7% inhibition | Thermococcus litoralis | |
Ca2+ | 0.01 mM, 45% inhibition | Thermococcus litoralis | |
Cs+ | 0.01 mM, 47% inhibition | Thermococcus litoralis | |
Cu2+ | 0.01 mM, 95% inhibition | Thermococcus litoralis | |
dithiothreitol | 1 mM, 22% inhibition; 1 mM, 42% inhibition | Thermococcus litoralis | |
EDTA | 1 mM, 45% inhibition | Thermococcus litoralis | |
Fe2+ | 0.01 mM, 33% inhibition | Thermococcus litoralis | |
Fe3+ | 0.01 mM, 99% inhibition | Thermococcus litoralis | |
iodoacetate | 1 mM, 1.1fold activation | Thermococcus litoralis | |
Isopropanol | 10% | Thermococcus litoralis | |
K+ | 0.01 mM, 24% inhibition | Thermococcus litoralis | |
La3+ | 0.01 mM, 17% inhibition | Thermococcus litoralis | |
Li+ | 0.01 mM, 51% inhibition | Thermococcus litoralis | |
Mg2+ | 0.01 mM, 8% inhibition | Thermococcus litoralis | |
Mn2+ | 0.01 mM, 54% inhibition | Thermococcus litoralis | |
mono-tert-butyl malonate | 25 mM, 14% inhibition | Thermococcus litoralis | |
N-(4-toluenesulfonyl)-DL-methionine | 25 mM, 14% inhibition | Thermococcus litoralis | |
N-(p-toluenesulfonyl)-DL-Met | 25 mM, 14% inhibition | Thermococcus litoralis | |
Na+ | 0.01 mM, 19% inhibition | Thermococcus litoralis | |
Ni2+ | 0.01 mM, 27% inhibition | Thermococcus litoralis | |
phenylmethylsulfonyl fluoride | 1 mM, 22% inhibition | Thermococcus litoralis | |
Sn4+ | 0.01 mM, 99% inhibition | Thermococcus litoralis | |
tosyl-L-lysine chloromethyl ketone | 25 mM, 7% inhibition | Thermococcus litoralis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 0.01 mM, 1.1fold activation | Thermococcus litoralis | |
Co2+ | 0.01 mM, 1.1fold activation. 0.1 mM, 1.3fold activation | Thermococcus litoralis | |
Zn2+ | 0.01 mM or 0.1 mM, 1.3fold activation | Thermococcus litoralis | |
Zn2+ | 0.01 mM, 1.3fold activation | Thermococcus litoralis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
4 * 43000, SDS-PAGE | Thermococcus litoralis |
43000 | - |
4 * 43000, SDS-PAGE, sedimentation studies | Thermococcus litoralis |
43814 | - |
4 * 43814, calculated from sequence | Thermococcus litoralis |
43815 | - |
4 * 43815, calculated from sequence | Thermococcus litoralis |
170000 | - |
gel filtration | Thermococcus litoralis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus litoralis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is most specific for substrates containing N-benzoyl- or N-chloroacetyl-amino acids, preferring substrates containing hydrophobic, uncharged, or weakly charged amino acids such as phenylalanine, methionine, and cysteine. It contains esterase activity with 4-nitrophenyl-palmitate and 4-nitrophenyllaurate, but not Phe-ethyl ester at 85°C | Thermococcus litoralis | ? | - |
? | |
additional information | the enzyme is most specific for substrates containing N-benzoyl- or N-chloroacetyl-amino acids, preferring substrates containing hydrophobic, uncharged, or weakly charged amino acids such as phenylalanine, methionine, and cysteine. No activity with N-benzoyl-D-phenylalanine, N-benzyloxycarbonyl-D-phenylalanine, tert-butyloxycarbonyl-D-phenylalanine, N-acetyl-D-tryptophan | Thermococcus litoralis | ? | - |
? | |
N-acetyl-L-cysteine + H2O | - |
Thermococcus litoralis | acetate + L-cysteine | - |
? | |
N-acetyl-L-methionine + H2O | - |
Thermococcus litoralis | acetate + L-methionine | - |
? | |
N-acetyl-L-methionine + H2O | 40.5% of the activity with N-acetyl-L-phenylalanine | Thermococcus litoralis | acetate + L-methionine | - |
? | |
N-acetyl-L-Phe methyl ester + H2O | the enzyme also shows esterase activity with N-acetyl-L-Phe methyl ester | Thermococcus litoralis | acetate + L-Phe methyl ester | - |
? | |
N-acetyl-L-phenylalanine + H2O | no activity with N-acetyl-D-phenylalanine | Thermococcus litoralis | acetate + L-phenylalanine | - |
? | |
N-acetyl-L-phenylalanine methyl ester + H2O | - |
Thermococcus litoralis | acetate + L-phenylalanine methyl ester | - |
? | |
N-acetyl-L-tryptophan + H2O | - |
Thermococcus litoralis | acetate + L-tryptophan | - |
? | |
N-benzoyl-L-phenylalanine + H2O | - |
Thermococcus litoralis | benzoate + L-phenylalanine | - |
? | |
N-benzoyl-L-phenylalanine + H2O | 735.7% of the activity with N-acetyl-L-phenylalanine | Thermococcus litoralis | benzoate + L-phenylalanine | - |
? | |
N-benzyloxycarbonyl-L-Phe-Gly + H2O | - |
Thermococcus litoralis | benzyl hydrogencarbonate + L-Phe-Gly | - |
? | |
N-benzyloxycarbonyl-L-phenylalanine + H2O | - |
Thermococcus litoralis | benzyl hydrogencarbonate + L-phenylalanine | - |
? | |
N-benzyloxycarbonyl-L-phenylalanine + H2O | 33.9% of the activity with N-acetyl-L-phenylalanine. no activity with N-benzyloxycarbonyl-D-phenylalanine | Thermococcus litoralis | benzyloxycarbonate + L-phenylalanine | - |
? | |
N-benzyloxycarbonyl-L-valine + H2O | - |
Thermococcus litoralis | benzyl hydrogencarbonate + L-valine | - |
? | |
N-chloroacetyl-L-phenylalanine + H2O | - |
Thermococcus litoralis | chloroacetate + L-phenylalanine | - |
? | |
N-tert-butyloxycarbonyl-L-phenylalanine + H2O | - |
Thermococcus litoralis | tert-butyloxycarboxylate + L-phenylalanine | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 43000, SDS-PAGE | Thermococcus litoralis |
homotetramer | 4 * 43000, SDS-PAGE, sedimentation studies | Thermococcus litoralis |
homotetramer | 4 * 43814, calculated from sequence | Thermococcus litoralis |
homotetramer | 4 * 43815, calculated from sequence | Thermococcus litoralis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
assay at | Thermococcus litoralis |
85 | - |
at pH 8.0 | Thermococcus litoralis |
85 | - |
1 h, Tris-HCl at pH 8.0 | Thermococcus litoralis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
half-life: 25 h | Thermococcus litoralis |
85 | - |
half-life: 1.7 h | Thermococcus litoralis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Thermococcus litoralis |
7.5 | - |
with Mes buffer | Thermococcus litoralis |
8 | - |
assay at | Thermococcus litoralis |
8 | - |
with Tris-HCl or phosphate buffers | Thermococcus litoralis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.5 | at least 70% of the activity is present between pH 6.5 and 9.5 | Thermococcus litoralis |