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Literature summary for 3.5.1.14 extracted from

  • Stocker, P.; Brunel, J.; de Rezende, L.; -do Amaral, A.; Morelli, X.; Roche, P.; Vidal, N.; Giardina, T.; Perrier, J.
    Aminoacylase 1-catalysed deacetylation of bioactives epoxides mycotoxin-derived mercapturates; 3,4-epoxyprecocenes as models of cytotoxic epoxides (2012), Biochimie, 94, 1668-1675.
    View publication on PubMed
Search for more literature for 3.5.1.14

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
0.15
-
 2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid analysis by HPLC, pH 7.6, 37°C Sus scrofa
0.2
-
 N-acetyl-L-Met analysis by HPLC, pH 7.6, 37°C Sus scrofa
0.2
-
 2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid analysis by HPLC, pH 7.6, 37°C Homo sapiens
0.2
-
 2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
0.3
-
 2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid analysis by HPLC, pH 7.6, 37°C Sus scrofa
0.3
-
 2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid analysis by HPLC, pH 7.6, 37°C Homo sapiens
0.3
-
 N-acetyl-L-Met analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
0.4
-
 N-acetyl-L-Cys analysis by HPLC, pH 7.6, 37°C Sus scrofa
0.4
-
 2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid analysis by HPLC, pH 7.6, 37°C Sus scrofa
0.4
-
 N-acetyl-L-Val analysis by HPLC, pH 7.6, 37°C Sus scrofa
0.5
-
 N-acetyl-L-Cys analysis by HPLC, pH 7.6, 37°C Homo sapiens
0.5
-
 N-acetyl-L-Met analysis by HPLC, pH 7.6, 37°C Homo sapiens
0.5
-
 2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid analysis by HPLC, pH 7.6, 37°C Homo sapiens
0.5
-
 N-acetyl-L-Cys analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
0.5
-
 2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
0.5
-
 N-acetyl-L-Val analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
1
-
 2-acetylamino-3-((3S,4S)-2-hydroxycyclooctylsulfanyl)-propionic acid analysis by HPLC, pH 7.6, 37°C Sus scrofa

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Sus scrofa P37111
-
-

Purification (Commentary)

Purification (Comment) Organism
commercial preparation Homo sapiens
commercial preparation Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid + H2O
-
 Homo sapiens S-[(1S,2S)-2-hydroxycyclohexyl]cysteine + acetate
-
 ?
2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid + H2O
-
 Sus scrofa S-[(1S,2S)-2-hydroxycyclohexyl]cysteine + acetate
-
 ?
2-acetylamino-3-((1S,2S)-2-hydroxycyclooctylsulfanyl)-propionic acid + H2O
-
 Sus scrofa S-[(1S,2S)-2-hydroxycyclooctyl]cysteine + acetate
-
 ?
2-acetylamino-3-((3S,4S)-3-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid + H2O
-
 Homo sapiens S-[(3S,4S)-3-hydroxy-2,2-dimethyl-3,4-dihydro-2H-chromen-4-yl]cysteine + acetate
-
 ?
2-acetylamino-3-((3S,4S)-3-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid + H2O
-
 Sus scrofa S-[(3S,4S)-3-hydroxy-2,2-dimethyl-3,4-dihydro-2H-chromen-4-yl]cysteine + acetate
-
 ?
2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid + H2O
-
 Homo sapiens S-[(1R,2R)-1-hydroxy-3-oxo-1,3-diphenylpropan-2-yl]cysteine + acetate
-
 ?
2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid + H2O
-
 Sus scrofa S-[(1R,2R)-1-hydroxy-3-oxo-1,3-diphenylpropan-2-yl]cysteine + acetate
-
 ?
N-acetyl-L-Cys + H2O
-
 Homo sapiens acetate + L-Cys
-
 ?
N-acetyl-L-Cys + H2O
-
 Sus scrofa L-Cys + acetate
-
 ?
N-acetyl-L-Met + H2O
-
 Homo sapiens acetate + L-Met
-
 ?
N-acetyl-L-Met + H2O
-
 Sus scrofa L-Met + acetate
-
 ?
N-acetyl-L-Val + H2O
-
 Sus scrofa L-Val + acetate
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Homo sapiens
homodimer
-
 Sus scrofa

Synonyms

Synonyms Comment Organism
aminoacylase 1
-
 Homo sapiens
aminoacylase 1
-
 Sus scrofa
N-acyl-L-amino acid hydrolase
-
 Homo sapiens
N-acyl-L-amino acid hydrolase
-
 Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7
-
 N-acetyl-L-Val analysis by HPLC, pH 7.6, 37°C Sus scrofa
2
-
 N-acetyl-L-Val analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
3
-
 2-acetylamino-3-((3S,4S)-2-hydroxycyclooctylsulfanyl)-propionic acid analysis by HPLC, pH 7.6, 37°C Sus scrofa
9
-
 N-acetyl-L-Cys analysis by HPLC, pH 7.6, 37°C Sus scrofa
18
-
 N-acetyl-L-Cys analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
19
-
 N-acetyl-L-Met analysis by HPLC, pH 7.6, 37°C Sus scrofa
19.5
-
 2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid analysis by HPLC, pH 7.6, 37°C Sus scrofa
21
-
 2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
26
-
 2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
30
-
 N-acetyl-L-Cys analysis by HPLC, pH 7.6, 37°C Homo sapiens
30
-
 2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid analysis by HPLC, pH 7.6, 37°C Sus scrofa
30
-
 N-acetyl-L-Met analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
45
-
 2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid analysis by HPLC, pH 7.6, 37°C Sus scrofa
50
-
 2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
65
-
 N-acetyl-L-Met analysis by HPLC, pH 7.6, 37°C Homo sapiens
80
-
 2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid analysis by HPLC, pH 7.6, 37°C Homo sapiens
120
-
 2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid analysis by HPLC, pH 7.6, 37°C Homo sapiens
150
-
 2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid analysis by HPLC, pH 7.6, 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3
-
 2-acetylamino-3-((3S,4S)-2-hydroxycyclooctylsulfanyl)-propionic acid analysis by HPLC, pH 7.6, 37°C Sus scrofa
4
-
 N-acetyl-L-Val analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
4.3
-
 N-acetyl-L-Val analysis by HPLC, pH 7.6, 37°C Sus scrofa
23
-
 N-acetyl-L-Cys analysis by HPLC, pH 7.6, 37°C Sus scrofa
36
-
 N-acetyl-L-Cys analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
49
-
 2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid analysis by HPLC, pH 7.6, 37°C Sus scrofa
52
-
 2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
60
-
 N-acetyl-L-Cys analysis by HPLC, pH 7.6, 37°C Homo sapiens
95
-
 N-acetyl-L-Met analysis by HPLC, pH 7.6, 37°C Sus scrofa
100
-
 2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid analysis by HPLC, pH 7.6, 37°C Sus scrofa
100
-
 N-acetyl-L-Met analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
110
-
 2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
130
-
 N-acetyl-L-Met analysis by HPLC, pH 7.6, 37°C Homo sapiens
240
-
 2-acetylamino-3-((1S,2S)-2-hydroxycyclohexylsulfanyl)-propionic acid analysis by HPLC, pH 7.6, 37°C Homo sapiens
270
-
 2-acetylamino-3-[(S)-1-((R)-hydroxyphenyl-methyl)-2-oxo-2-phenyl-ethylsulfanyl]-propionic acid analysis by HPLC, pH 7.6, 37°C Homo sapiens
300
-
 2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid analysis by HPLC, pH 7.6, 37°C Sus scrofa
500
-
 2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid analysis by TNBS (2,4,6-trinitrobenzenesulphonic acid) assay, pH 7.6, 37°C Sus scrofa
750
-
 2-acetylamino-3-((1S,2S)-2-hydroxy-2,2-dimethyl-chroman-4-ylsulfanyl)-propionic acid analysis by HPLC, pH 7.6, 37°C Homo sapiens