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Literature summary for 3.5.1.14 extracted from

  • Dong, T.; Zhao, L.; Huang, Y.; Tan, X.
    Preparation of cross-linked aggregates of aminoacylase from Aspergillus melleus by using bovine serum albumin as an inert additive (2010), Biores. Technol., 101, 6569-6571.
    View publication on PubMed
Search for more literature for 3.5.1.14

Protein Variants

Protein Variants Comment Organism
additional information preparation of cross-linked aggregates of aminoacylase by using bovine serum albumin as an inert additive, cross-linking is conducted at varying glutaraldehyde to enzyme ratio. Cross-linked enzyme aggregates retain 82% of maximal activity in presence of bovine serum albumin, and 24% in its absence Aspergillus melleus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.964
-
 N-acetyl-L-methionine pH 8.0, 40°C, native enzyme Aspergillus melleus
1.497
-
 N-acetyl-L-methionine pH 8.0, 40°C, immobilized, cross-linked enzyme-bovine serum albumin aggregate Aspergillus melleus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Aspergillus melleus aminoacylase catalyzes the hydrolysis of L-acyl-amino acids to produce the corresponding L-amino acid ?
-
 ?
N-acetyl-L-methionine + H2O Aspergillus melleus
-
 acetate + L-methionine
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus melleus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Aspergillus melleus
-

Storage Stability

Storage Stability Organism
47°C, pH 8.0, 0.2% w/v, 0.1 M sodium phosphate buffer, no and 23% remaining activity for the free aminoacylase and the immobilized, cross-linked enzyme-bovine serum albumin aggregate, respectively, after 24 h Aspergillus melleus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information aminoacylase catalyzes the hydrolysis of L-acyl-amino acids to produce the corresponding L-amino acid Aspergillus melleus ?
-
 ?
N-acetyl-L-methionine + H2O
-
 Aspergillus melleus acetate + L-methionine
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 assay at Aspergillus melleus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Aspergillus melleus