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Literature summary for 3.5.1.14 extracted from
- Enantioselective synthesis of L-homophenylalanine by whole cells of recombinant Escherichia coli expressing L-aminoacylase and N-acylamino acid racemase genes from Deinococcus radiodurans BCRC12827 (2006), Biotechnol. Prog., 22, 1578-1584.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Deinococcus radiodurans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Deinococcus radiodurans |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
- |
Deinococcus radiodurans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Deinococcus radiodurans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Deinococcus radiodurans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
1.74 U/mg in phosphate buffer and 0.88 U/mg in 0.1M Tris-HCl buffer | Deinococcus radiodurans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, pH 8 | Deinococcus radiodurans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-D-Homophenylalanine + H2O | - |
Deinococcus radiodurans | acetate + D-Homophenylalanine | - |
? | |
| N-acetyl-L-Homophenylalanine + H2O | - |
Deinococcus radiodurans | acetate + L-Homophenylalanine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LAA | - |
Deinococcus radiodurans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Deinococcus radiodurans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 90 | - |
Deinococcus radiodurans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Deinococcus radiodurans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.7 | 10.8 | using different buffers containing N-acetyl-D-Homophenylalanine or N-acetyl-L-Homophenylalanine | Deinococcus radiodurans |
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Release 2023.1 (January 2023)
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