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Literature summary for 3.5.1.14 extracted from

  • Koreishi, M.; Zhang, D.; Imanaka, H.; Imamura, K.; Adachi, S.; Matsuno, R.; Nakanishi, K.
    A novel acylase from Streptomyces mobaraensis that efficiently catalyzes hydrolysis/synthesis of capsaicins as well as N-acyl-L-amino acids and N-acyl-peptides (2006), J. Agric. Food Chem., 54, 72-78.
    View publication on PubMed

General Stability

General Stability Organism
Co2+ activates and stabilizes Streptomyces mobaraensis

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 24% inhibition Streptomyces mobaraensis
EDTA 13% inhibition Streptomyces mobaraensis
additional information no effects by GSH, L-Cys, (NH4)6Mo7O24, PMSF, PCMB, DTT, MgSO4, AgNO3, CaCl2, FeSO4, ZnCl2, and CuSO4 Streptomyces mobaraensis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates and stabilizes, 148% activity at 0.1 mM, tightly bound to the enzyme Streptomyces mobaraensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
1 * 61000 + 1 * 19000, SDS-PAGE, the enzyme is active only as complete heterodimer Streptomyces mobaraensis
61000
-
1 * 61000 + 1 * 19000, SDS-PAGE, the enzyme is active only as complete heterodimer Streptomyces mobaraensis
75000
-
gel filtration Streptomyces mobaraensis

Organism

Organism UniProt Comment Textmining
Streptomyces mobaraensis
-
i.e. Streptomyces luteoreticuli, strains NBRC/IFO 13819 and 13422
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 1300fold by ammonium sulfate fractionation, dialysis, ion exchange chromatography, hydroxyapatite chromatography, and a second step of dialysis Streptomyces mobaraensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
22
-
purified enzyme, substrate N-lauroyl-L-tryptophan Streptomyces mobaraensis
25
-
purified enzyme, substrate N-lauroyl-L-phenylalanine Streptomyces mobaraensis
70
-
purified enzyme, substrate N-lauroyl-L-glutaminyl-L-lysine Streptomyces mobaraensis
87
-
purified enzyme, substrate N-lauroyl-L-valine Streptomyces mobaraensis
90
-
purified enzyme, substrate N-lauroyl-L-glutamyl-glycine Streptomyces mobaraensis
90
-
purified enzyme, substrate N-lauroyl-L-isoleucine Streptomyces mobaraensis
110
-
purified enzyme, substrate N-lauroyl-L-glutaminyl-glycine Streptomyces mobaraensis
118
-
purified enzyme, substrate N-lauroyl-L-histidine Streptomyces mobaraensis
129
-
purified enzyme, substrate N-lauroyl-L-leucine Streptomyces mobaraensis
138
-
purified enzyme, substrate N-lauroyl-L-aspartic acid Streptomyces mobaraensis
145
-
purified enzyme, substrate N-lauroyl-L-tyrosine Streptomyces mobaraensis
177
-
purified enzyme, substrate N-lauroyl-L-methionine Streptomyces mobaraensis
194
-
purified enzyme, substrate N-lauroyl-L-arginine Streptomyces mobaraensis
196
-
purified enzyme, substrate N-lauroyl-L-glutamic acid Streptomyces mobaraensis
210
-
purified enzyme, substrate capsaicin Streptomyces mobaraensis
221
-
purified enzyme, substrate N-lauroyl-L-glutamine Streptomyces mobaraensis
222
-
purified enzyme, substrate N-lauroyl-glycine Streptomyces mobaraensis
227
-
purified enzyme, substrate N-lauroyl-L-asparagine Streptomyces mobaraensis
230
-
purified enzyme, substrate N-lauroyl-L-threonine Streptomyces mobaraensis
236
-
purified enzyme, substrate N-lauroyl-L-alanine Streptomyces mobaraensis
244
-
purified enzyme, substrate N-lauroyl-L-lysine Streptomyces mobaraensis
295
-
purified enzyme, substrate N-lauroyl-L-serine Streptomyces mobaraensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8-methyl-N-vanillyl-6-nonenamide + H2O i.e. capsaicin Streptomyces mobaraensis ?
-
?
additional information substrate synthesis in an aqueous/n-hexane biphasic system Streptomyces mobaraensis ?
-
?
N-lauroyl-glycine + H2O
-
Streptomyces mobaraensis laurate + glycine
-
?
N-lauroyl-L-alanine + H2O
-
Streptomyces mobaraensis laurate + L-alanine
-
?
N-lauroyl-L-arginine + H2O
-
Streptomyces mobaraensis laurate + L-arginine
-
?
N-lauroyl-L-asparagine + H2O
-
Streptomyces mobaraensis laurate + L-asparagine
-
?
N-lauroyl-L-aspartate + H2O
-
Streptomyces mobaraensis laurate + L-aspartate
-
?
N-lauroyl-L-glutamate + H2O
-
Streptomyces mobaraensis laurate + L-glutamate
-
?
N-lauroyl-L-glutamine + H2O
-
Streptomyces mobaraensis laurate + L-glutamine
-
?
N-lauroyl-L-glutaminyl-glycine + H2O
-
Streptomyces mobaraensis laurate + L-glutaminyl-glycine
-
?
N-lauroyl-L-glutaminyl-L-lysine + H2O
-
Streptomyces mobaraensis laurate + L-glutaminyl-L-lysine
-
?
N-lauroyl-L-glutamyl-glycine + H2O
-
Streptomyces mobaraensis laurate + L-glutamyl-glycine
-
?
N-lauroyl-L-histidine + H2O
-
Streptomyces mobaraensis laurate + L-histidine
-
?
N-lauroyl-L-isoleucine + H2O
-
Streptomyces mobaraensis laurate + L-isoleucine
-
?
N-lauroyl-L-leucine + H2O
-
Streptomyces mobaraensis laurate + L-leucine
-
?
N-lauroyl-L-methionine + H2O
-
Streptomyces mobaraensis laurate + L-methionine
-
?
N-lauroyl-L-phenylalanine + H2O
-
Streptomyces mobaraensis laurate + L-phenylalanine
-
?
N-lauroyl-L-serine + H2O best substrate Streptomyces mobaraensis laurate + L-serine
-
?
N-lauroyl-L-threonine + H2O
-
Streptomyces mobaraensis laurate + L-threonine
-
?
N-lauroyl-L-tryptophan + H2O
-
Streptomyces mobaraensis laurate + L-tryptophan
-
?
N-lauroyl-L-tyrosine + H2O
-
Streptomyces mobaraensis laurate + L-tyrosine
-
?
N-lauroyl-L-valine + H2O
-
Streptomyces mobaraensis laurate + L-valine
-
?
Nalpha-lauroyl-L-lysine + H2O
-
Streptomyces mobaraensis laurate + L-lysine
-
?
Nepsilon-lauroyl-L-lysine + H2O
-
Streptomyces mobaraensis laurate + L-lysine
-
?

Subunits

Subunits Comment Organism
dimer 1 * 61000 + 1 * 19000, SDS-PAGE, the enzyme is active only as complete heterodimer Streptomyces mobaraensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Streptomyces mobaraensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
1 h, pH 7.8, stable, above 55°C the enzyme is stable only in presence of Co2+ Streptomyces mobaraensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Streptomyces mobaraensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 50 mm Tris, 0.5 mM Co2+, 37°C, stable Streptomyces mobaraensis