BRENDA - Enzyme Database
show all sequences of 3.5.1.14

A novel acylase from Streptomyces mobaraensis that efficiently catalyzes hydrolysis/synthesis of capsaicins as well as N-acyl-L-amino acids and N-acyl-peptides

Koreishi, M.; Zhang, D.; Imanaka, H.; Imamura, K.; Adachi, S.; Matsuno, R.; Nakanishi, K.; J. Agric. Food Chem. 54, 72-78 (2006) View publication on PubMed

Data extracted from this reference:

General Stability
General Stability
Organism
Co2+ activates and stabilizes
Streptomyces mobaraensis
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
24% inhibition
Streptomyces mobaraensis
EDTA
13% inhibition
Streptomyces mobaraensis
additional information
no effects by GSH, L-Cys, (NH4)6Mo7O24, PMSF, PCMB, DTT, MgSO4, AgNO3, CaCl2, FeSO4, ZnCl2, and CuSO4
Streptomyces mobaraensis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
activates and stabilizes, 148% activity at 0.1 mM, tightly bound to the enzyme
Streptomyces mobaraensis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
19000
-
1 * 61000 + 1 * 19000, SDS-PAGE, the enzyme is active only as complete heterodimer
Streptomyces mobaraensis
61000
-
1 * 61000 + 1 * 19000, SDS-PAGE, the enzyme is active only as complete heterodimer
Streptomyces mobaraensis
75000
-
gel filtration
Streptomyces mobaraensis
Organism
Organism
UniProt
Commentary
Textmining
Streptomyces mobaraensis
-
i.e. Streptomyces luteoreticuli, strains NBRC/IFO 13819 and 13422
-
Purification (Commentary)
Purification (Commentary)
Organism
native enzyme 1300fold by ammonium sulfate fractionation, dialysis, ion exchange chromatography, hydroxyapatite chromatography, and a second step of dialysis
Streptomyces mobaraensis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
22
-
purified enzyme, substrate N-lauroyl-L-tryptophan
Streptomyces mobaraensis
25
-
purified enzyme, substrate N-lauroyl-L-phenylalanine
Streptomyces mobaraensis
70
-
purified enzyme, substrate N-lauroyl-L-glutaminyl-L-lysine
Streptomyces mobaraensis
87
-
purified enzyme, substrate N-lauroyl-L-valine
Streptomyces mobaraensis
90
-
purified enzyme, substrate N-lauroyl-L-glutamyl-glycine
Streptomyces mobaraensis
90
-
purified enzyme, substrate N-lauroyl-L-isoleucine
Streptomyces mobaraensis
110
-
purified enzyme, substrate N-lauroyl-L-glutaminyl-glycine
Streptomyces mobaraensis
118
-
purified enzyme, substrate N-lauroyl-L-histidine
Streptomyces mobaraensis
129
-
purified enzyme, substrate N-lauroyl-L-leucine
Streptomyces mobaraensis
138
-
purified enzyme, substrate N-lauroyl-L-aspartic acid
Streptomyces mobaraensis
145
-
purified enzyme, substrate N-lauroyl-L-tyrosine
Streptomyces mobaraensis
177
-
purified enzyme, substrate N-lauroyl-L-methionine
Streptomyces mobaraensis
194
-
purified enzyme, substrate N-lauroyl-L-arginine
Streptomyces mobaraensis
196
-
purified enzyme, substrate N-lauroyl-L-glutamic acid
Streptomyces mobaraensis
210
-
purified enzyme, substrate capsaicin
Streptomyces mobaraensis
221
-
purified enzyme, substrate N-lauroyl-L-glutamine
Streptomyces mobaraensis
222
-
purified enzyme, substrate N-lauroyl-glycine
Streptomyces mobaraensis
227
-
purified enzyme, substrate N-lauroyl-L-asparagine
Streptomyces mobaraensis
230
-
purified enzyme, substrate N-lauroyl-L-threonine
Streptomyces mobaraensis
236
-
purified enzyme, substrate N-lauroyl-L-alanine
Streptomyces mobaraensis
244
-
purified enzyme, substrate N-lauroyl-L-lysine
Streptomyces mobaraensis
295
-
purified enzyme, substrate N-lauroyl-L-serine
Streptomyces mobaraensis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
8-methyl-N-vanillyl-6-nonenamide + H2O
i.e. capsaicin
669031
Streptomyces mobaraensis
?
-
-
-
?
additional information
substrate synthesis in an aqueous/n-hexane biphasic system
669031
Streptomyces mobaraensis
?
-
-
-
?
N-lauroyl-glycine + H2O
-
669031
Streptomyces mobaraensis
laurate + glycine
-
-
-
?
N-lauroyl-L-alanine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-alanine
-
-
-
?
N-lauroyl-L-arginine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-arginine
-
-
-
?
N-lauroyl-L-asparagine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-asparagine
-
-
-
?
N-lauroyl-L-aspartate + H2O
-
669031
Streptomyces mobaraensis
laurate + L-aspartate
-
-
-
?
N-lauroyl-L-glutamate + H2O
-
669031
Streptomyces mobaraensis
laurate + L-glutamate
-
-
-
?
N-lauroyl-L-glutamine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-glutamine
-
-
-
?
N-lauroyl-L-glutaminyl-glycine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-glutaminyl-glycine
-
-
-
?
N-lauroyl-L-glutaminyl-L-lysine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-glutaminyl-L-lysine
-
-
-
?
N-lauroyl-L-glutamyl-glycine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-glutamyl-glycine
-
-
-
?
N-lauroyl-L-histidine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-histidine
-
-
-
?
N-lauroyl-L-isoleucine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-isoleucine
-
-
-
?
N-lauroyl-L-leucine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-leucine
-
-
-
?
N-lauroyl-L-methionine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-methionine
-
-
-
?
N-lauroyl-L-phenylalanine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-phenylalanine
-
-
-
?
N-lauroyl-L-serine + H2O
best substrate
669031
Streptomyces mobaraensis
laurate + L-serine
-
-
-
?
N-lauroyl-L-threonine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-threonine
-
-
-
?
N-lauroyl-L-tryptophan + H2O
-
669031
Streptomyces mobaraensis
laurate + L-tryptophan
-
-
-
?
N-lauroyl-L-tyrosine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-tyrosine
-
-
-
?
N-lauroyl-L-valine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-valine
-
-
-
?
Nalpha-lauroyl-L-lysine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-lysine
-
-
-
?
Nepsilon-lauroyl-L-lysine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-lysine
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
1 * 61000 + 1 * 19000, SDS-PAGE, the enzyme is active only as complete heterodimer
Streptomyces mobaraensis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
55
-
-
Streptomyces mobaraensis
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
1 h, pH 7.8, stable, above 55°C the enzyme is stable only in presence of Co2+
Streptomyces mobaraensis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Streptomyces mobaraensis
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5
8
50 mm Tris, 0.5 mM Co2+, 37°C, stable
Streptomyces mobaraensis
General Stability (protein specific)
General Stability
Organism
Co2+ activates and stabilizes
Streptomyces mobaraensis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
24% inhibition
Streptomyces mobaraensis
EDTA
13% inhibition
Streptomyces mobaraensis
additional information
no effects by GSH, L-Cys, (NH4)6Mo7O24, PMSF, PCMB, DTT, MgSO4, AgNO3, CaCl2, FeSO4, ZnCl2, and CuSO4
Streptomyces mobaraensis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
activates and stabilizes, 148% activity at 0.1 mM, tightly bound to the enzyme
Streptomyces mobaraensis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
19000
-
1 * 61000 + 1 * 19000, SDS-PAGE, the enzyme is active only as complete heterodimer
Streptomyces mobaraensis
61000
-
1 * 61000 + 1 * 19000, SDS-PAGE, the enzyme is active only as complete heterodimer
Streptomyces mobaraensis
75000
-
gel filtration
Streptomyces mobaraensis
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme 1300fold by ammonium sulfate fractionation, dialysis, ion exchange chromatography, hydroxyapatite chromatography, and a second step of dialysis
Streptomyces mobaraensis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
22
-
purified enzyme, substrate N-lauroyl-L-tryptophan
Streptomyces mobaraensis
25
-
purified enzyme, substrate N-lauroyl-L-phenylalanine
Streptomyces mobaraensis
70
-
purified enzyme, substrate N-lauroyl-L-glutaminyl-L-lysine
Streptomyces mobaraensis
87
-
purified enzyme, substrate N-lauroyl-L-valine
Streptomyces mobaraensis
90
-
purified enzyme, substrate N-lauroyl-L-glutamyl-glycine
Streptomyces mobaraensis
90
-
purified enzyme, substrate N-lauroyl-L-isoleucine
Streptomyces mobaraensis
110
-
purified enzyme, substrate N-lauroyl-L-glutaminyl-glycine
Streptomyces mobaraensis
118
-
purified enzyme, substrate N-lauroyl-L-histidine
Streptomyces mobaraensis
129
-
purified enzyme, substrate N-lauroyl-L-leucine
Streptomyces mobaraensis
138
-
purified enzyme, substrate N-lauroyl-L-aspartic acid
Streptomyces mobaraensis
145
-
purified enzyme, substrate N-lauroyl-L-tyrosine
Streptomyces mobaraensis
177
-
purified enzyme, substrate N-lauroyl-L-methionine
Streptomyces mobaraensis
194
-
purified enzyme, substrate N-lauroyl-L-arginine
Streptomyces mobaraensis
196
-
purified enzyme, substrate N-lauroyl-L-glutamic acid
Streptomyces mobaraensis
210
-
purified enzyme, substrate capsaicin
Streptomyces mobaraensis
221
-
purified enzyme, substrate N-lauroyl-L-glutamine
Streptomyces mobaraensis
222
-
purified enzyme, substrate N-lauroyl-glycine
Streptomyces mobaraensis
227
-
purified enzyme, substrate N-lauroyl-L-asparagine
Streptomyces mobaraensis
230
-
purified enzyme, substrate N-lauroyl-L-threonine
Streptomyces mobaraensis
236
-
purified enzyme, substrate N-lauroyl-L-alanine
Streptomyces mobaraensis
244
-
purified enzyme, substrate N-lauroyl-L-lysine
Streptomyces mobaraensis
295
-
purified enzyme, substrate N-lauroyl-L-serine
Streptomyces mobaraensis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
8-methyl-N-vanillyl-6-nonenamide + H2O
i.e. capsaicin
669031
Streptomyces mobaraensis
?
-
-
-
?
additional information
substrate synthesis in an aqueous/n-hexane biphasic system
669031
Streptomyces mobaraensis
?
-
-
-
?
N-lauroyl-glycine + H2O
-
669031
Streptomyces mobaraensis
laurate + glycine
-
-
-
?
N-lauroyl-L-alanine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-alanine
-
-
-
?
N-lauroyl-L-arginine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-arginine
-
-
-
?
N-lauroyl-L-asparagine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-asparagine
-
-
-
?
N-lauroyl-L-aspartate + H2O
-
669031
Streptomyces mobaraensis
laurate + L-aspartate
-
-
-
?
N-lauroyl-L-glutamate + H2O
-
669031
Streptomyces mobaraensis
laurate + L-glutamate
-
-
-
?
N-lauroyl-L-glutamine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-glutamine
-
-
-
?
N-lauroyl-L-glutaminyl-glycine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-glutaminyl-glycine
-
-
-
?
N-lauroyl-L-glutaminyl-L-lysine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-glutaminyl-L-lysine
-
-
-
?
N-lauroyl-L-glutamyl-glycine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-glutamyl-glycine
-
-
-
?
N-lauroyl-L-histidine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-histidine
-
-
-
?
N-lauroyl-L-isoleucine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-isoleucine
-
-
-
?
N-lauroyl-L-leucine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-leucine
-
-
-
?
N-lauroyl-L-methionine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-methionine
-
-
-
?
N-lauroyl-L-phenylalanine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-phenylalanine
-
-
-
?
N-lauroyl-L-serine + H2O
best substrate
669031
Streptomyces mobaraensis
laurate + L-serine
-
-
-
?
N-lauroyl-L-threonine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-threonine
-
-
-
?
N-lauroyl-L-tryptophan + H2O
-
669031
Streptomyces mobaraensis
laurate + L-tryptophan
-
-
-
?
N-lauroyl-L-tyrosine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-tyrosine
-
-
-
?
N-lauroyl-L-valine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-valine
-
-
-
?
Nalpha-lauroyl-L-lysine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-lysine
-
-
-
?
Nepsilon-lauroyl-L-lysine + H2O
-
669031
Streptomyces mobaraensis
laurate + L-lysine
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
1 * 61000 + 1 * 19000, SDS-PAGE, the enzyme is active only as complete heterodimer
Streptomyces mobaraensis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
55
-
-
Streptomyces mobaraensis
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
1 h, pH 7.8, stable, above 55°C the enzyme is stable only in presence of Co2+
Streptomyces mobaraensis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Streptomyces mobaraensis
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5
8
50 mm Tris, 0.5 mM Co2+, 37°C, stable
Streptomyces mobaraensis
Other publictions for EC 3.5.1.14
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Protein Variants
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
kcat/KM [mM/s]
kcat/KM [mM/s] (protein specific)
752619
Adenan
-
Characterization of thermosta ...
Geobacillus sp., Geobacillus sp. SZN
Asia Pac. J. Mol. Biol. Biotechnol.
27
1-9
2019
-
-
-
-
-
1
6
-
-
4
1
-
-
2
-
-
1
-
-
-
1
-
2
1
-
1
1
4
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
6
-
-
-
4
1
-
-
-
-
1
-
-
1
-
2
1
1
1
4
-
1
1
-
-
-
-
-
-
-
-
755482
Cheng
Expressional divergence of th ...
Helicoverpa assulta, Helicoverpa armigera
Sci. Rep.
7
8721
2017
-
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
3
-
-
752480
Anila
A cysteine-specific fluoresce ...
Homo sapiens
Anal. Chem.
88
12161-12168
2016
-
2
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
1
-
2
1
-
-
-
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
733955
Shi
Overexpression of aminoacylase ...
Homo sapiens
Hum. Pathol.
44
1089-1097
2013
-
-
-
-
1
-
-
-
1
-
-
-
-
3
-
-
-
-
-
4
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
719017
Stocker
Aminoacylase 1-catalysed deace ...
Homo sapiens, Sus scrofa
Biochimie
94
1668-1675
2012
-
-
-
-
-
-
-
18
-
-
-
-
-
8
-
-
2
-
-
1
-
-
12
2
4
-
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
18
-
-
-
-
-
-
-
2
-
1
-
-
12
2
-
-
-
18
-
-
-
-
-
-
-
-
18
18
719598
Kuhns
A lepidopteran aminoacylase (L ...
Heliothis virescens
Insect Biochem. Mol. Biol.
42
32-40
2012
-
-
-
-
-
-
2
-
-
1
2
-
-
18
-
-
1
-
-
5
1
-
1
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
2
-
-
-
-
1
-
5
1
-
1
1
-
-
-
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Refolding intermediate of guan ...
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670454
Groger
The first aminoacylase-catalyz ...
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670918
Youshko
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Application of aminoacylase I ...
Aspergillus melleus
Tetrahedron
15
1933-1936
2004
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1
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Crystal structure of D-aminoac ...
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Curley
Purification and characterisat ...
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2
1
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1
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D'Ambrosio
Probing the dimeric structure ...
Sus scrofa
Biochemistry
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2003
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658227
Durand
Rat kidney acylase I: further ...
Rattus norvegicus
Biochimie
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2003
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3
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Natsch
A specific bacterial aminoacyl ...
Corynebacterium striatum, Corynebacterium striatum AX20
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2003
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657442
Hsu
Crystallization and preliminar ...
Alcaligenes faecalis
Acta Crystallogr. Sect. D
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2002
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724947
Toogood
A thermostable L-aminoacylase ...
Thermococcus litoralis
Extremophiles
6
111-122
2002
1
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2
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26
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4
5
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4
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27
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17
4
3
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2
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4
1
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726571
Hollingsworth
Crystallization and preliminar ...
Thermococcus litoralis
Acta Crystallogr. Sect. D
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507-510
2002
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1
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657573
German
-
Isolation and characterization ...
Streptomyces olivoreticuli
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1
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1
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1
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-
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721329
Ishikawa
Novel bifunctional hyperthermo ...
Pyrococcus horikoshii, Pyrococcus horikoshii OT-3
Appl. Environ. Microbiol.
67
673-679
2001
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1
1
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3
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2
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4
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21
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12
1
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4
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2
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1
1
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3
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2
2
4
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4
1
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-
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12
1
-
-
-
4
-
-
-
-
-
-
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-
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288874
Tamura
Noncompetitive, reversible inh ...
Aspergillus oryzae, Sus scrofa
Arch. Biochem. Biophys.
379
261-266
2000
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-
-
-
-
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6
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2
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4
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4
2
2
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1
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6
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2
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4
-
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4
2
-
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-
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1
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-
-
-
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288873
Wang
Preparation and properties of ...
Aspergillus oryzae, Sus scrofa
Appl. Biochem. Biotechnol.
76
183-191
1999
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2
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-
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2
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2
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5
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3
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1
6
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1
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1
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2
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2
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2
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3
-
1
6
-
1
-
1
-
2
-
-
-
-
-
-
-
-
-
288871
Uttamsingh
Acylase I-catalyzed deacetylat ...
Homo sapiens, Rattus norvegicus, Sus scrofa
Chem. Res. Toxicol.
11
801-809
1998
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1
-
-
-
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2
7
2
1
2
3
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11
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1
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-
8
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19
2
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-
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-
-
1
-
-
-
-
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-
-
1
-
-
-
-
-
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2
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7
2
1
2
3
-
-
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1
-
8
-
-
19
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
288872
Wakayama
-
Purification and characterizat ...
Aspergillus oryzae, Bacillus sp. (in: Bacteria), Stenotrophomonas maltophilia, Sus scrofa
J. Ferment. Bioeng.
85
278-282
1998
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4
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-
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10
2
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9
3
4
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4
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1
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-
-
-
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39
3
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1
-
-
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2
1
1
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-
-
-
-
4
-
-
-
-
-
-
10
-
2
-
9
3
4
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-
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1
-
-
-
-
39
3
1
-
-
-
2
1
1
-
-
-
-
-
-
-
288870
Giardina
The hog intestinal mucosa acyl ...
Bos taurus, Sus scrofa
Biochimie
79
265-273
1997
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-
-
-
-
1
4
1
1
3
1
2
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2
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1
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2
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1
5
2
4
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
4
-
1
1
3
1
2
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-
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1
-
2
-
1
5
2
1
-
1
-
1
-
1
-
-
-
-
-
-
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664342
Honda
Heterologous protein productio ...
Pseudomonas sp.
Biosci. Biotechnol. Biochem.
61
948-955
1997
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1
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-
-
-
-
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2
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1
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5
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1
1
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-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
5
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288869
Yang
Kinetics of inhibition of amin ...
Sus scrofa
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48
532-538
1996
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-
-
-
-
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2
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1
-
1
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10
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-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
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2
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-
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1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288868
Palm
Aminoacylase I from porcine ki ...
Homo sapiens, Sus scrofa
J. Protein Chem.
14
233-240
1995
-
-
-
-
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5
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1
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2
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4
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1
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-
-
-
-
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-
-
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-
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2
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1
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2
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-
4
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-
-
-
-
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-
-
-
-
-
-
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288867
Yang
Purification and characterizat ...
Alcaligenes sp., Aspergillus oryzae, Bacillus sp. (in: Bacteria), Cercidium texanum, Sus scrofa, Aspergillus oryzae No.9
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58
204-205
1994
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3
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-
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10
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1
8
4
5
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12
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1
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-
1
-
-
18
4
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2
-
3
-
2
1
1
-
-
-
-
-
3
-
-
-
-
-
-
10
-
-
1
8
4
5
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-
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1
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1
-
-
18
4
2
-
3
-
2
1
1
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-
-
-
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-
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31930
Moriguchi
Purification and characterizat ...
Alcaligenes sp.
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57
1145-1148
1993
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-
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-
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13
5
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2
1
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1
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1
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-
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8
1
2
1
1
1
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1
-
1
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-
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-
-
-
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-
-
-
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13
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5
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2
1
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1
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-
-
-
8
1
1
1
1
-
1
-
1
-
-
-
-
-
-
-
288866
Sakanyan
Gene cloning, sequence analysi ...
Aspergillus oryzae, Bacillus sp. (in: Bacteria), Homo sapiens, Sus scrofa
Appl. Environ. Microbiol.
59
3878-3888
1993
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3
1
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4
3
4
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15
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1
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-
-
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35
1
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1
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-
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-
-
-
-
-
-
-
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3
1
-
-
-
-
-
-
-
-
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4
3
4
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-
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1
-
-
-
-
35
1
1
-
-
-
-
-
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-
-
-
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-
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659675
Rosenberg
Studies directed toward the de ...
Aspergillus niger
J. Med. Chem.
36
449-459
1993
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1
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-
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1
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-
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1
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1
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1
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1
-
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1
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288863
Cho
-
Thermostable aminoacylase from ...
Aspergillus oryzae, Bacillus sp. (in: Bacteria), Sus scrofa
Agric. Biol. Chem.
51
2793-2800
1987
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3
-
-
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1
8
2
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7
7
3
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3
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3
-
-
-
-
-
24
3
-
1
-
1
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3
-
1
-
-
-
-
-
3
-
-
-
-
1
-
8
-
2
-
7
7
3
-
-
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3
-
-
-
-
24
3
1
-
1
-
3
-
1
-
-
-
-
-
-
-
288864
Henseling
Unusual solvent isotope effect ...
Sus scrofa
FEBS Lett.
219
27-30
1987
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1
-
-
-
-
-
7
-
-
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1
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4
-
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1
-
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2
-
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4
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1
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-
-
-
-
-
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-
-
-
-
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1
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-
-
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7
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1
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1
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2
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4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
760213
Keller
-
Enzymatic resolution of 2-tri ...
Sus scrofa
Tetrahedron Lett.
27
1249-1250
1986
-
-
-
-
-
-
-
-
-
-
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1
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1
-
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2
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2
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-
-
-
-
-
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-
-
-
-
-
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-
-
-
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-
-
-
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-
1
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-
2
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-
-
-
-
-
-
-
-
-
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-
-
-
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288860
Gade
Purification, characterization ...
Bos taurus, Sus scrofa
Biochim. Biophys. Acta
662
86-93
1981
-
-
-
-
-
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4
1
-
2
2
2
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2
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1
-
-
1
-
1
24
2
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
1
-
2
2
2
-
-
-
1
-
1
-
1
24
2
-
-
-
-
3
1
-
-
-
-
-
-
-
-
288861
Gilles
-
Co2+-substituted acylamino aci ...
Aspergillus oryzae
Z. Naturforsch. C
36
751-754
1981
-
-
-
-
-
-
2
-
-
2
-
1
-
1
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
2
-
1
-
-
-
-
-
-
-
-
12
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
288862
Kumpe
Studies on the Zn2+/Co2+ excha ...
Sus scrofa
Z. Naturforsch. C
36
951-955
1981
-
-
-
-
-
-
3
-
-
2
-
1
-
1
-
-
1
-
-
1
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
2
-
1
-
-
-
1
-
1
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
288857
Gentzen
Aminoacylase from Aspergillus ...
Aspergillus oryzae, Sus scrofa
Z. Naturforsch. C
35
544-550
1980
-
-
-
-
-
-
8
-
-
4
4
2
-
12
-
-
1
-
-
4
-
1
5
1
-
-
-
2
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
4
4
2
-
-
-
1
-
4
-
1
5
1
-
-
2
-
3
-
-
-
-
-
-
-
-
-
288858
Sugie
-
Optical resolution of DL-amino ...
Streptomyces sp., no activity in Streptomyces olivaceus, no activity in Streptomyces olivaceus IFO 12805
Agric. Biol. Chem.
44
1089-1095
1980
-
2
-
-
-
-
4
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
11
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288859
Szajani
-
Comparative studies on soluble ...
Aspergillus oryzae, Sus scrofa
J. Appl. Biochem.
2
72-80
1980
-
1
-
-
-
-
-
2
-
-
-
2
-
2
-
-
1
-
-
1
-
-
8
1
-
2
1
1
-
4
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
2
-
-
-
1
-
1
-
-
8
1
2
1
1
-
4
-
1
-
-
-
-
-
-
-
288856
Koerdel
Chemical investigations on pig ...
Sus scrofa
Biochim. Biophys. Acta
445
446-457
1976
-
-
-
-
-
-
5
-
-
-
3
1
-
6
-
-
1
-
-
2
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
3
1
-
-
-
1
-
2
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288855
Matsumoto
Amidohydrolases for N-short an ...
Mycobacterium sp.
J. Biochem.
72
269-279
1972
3
-
-
-
-
-
6
2
-
5
2
1
-
1
-
-
1
-
-
-
-
1
8
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
-
6
-
2
-
5
2
1
-
-
-
1
-
-
-
1
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
288854
Park
An acylase system related to t ...
Streptomyces sp., Lactiplantibacillus plantarum, Sus scrofa
J. Biol. Chem.
235
3193-3197
1960
-
-
-
-
-
-
2
-
-
-
-
2
-
3
-
-
-
-
-
-
-
-
13
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
13
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
288853
Fones
Hydrolysis of N-acyl derivativ ...
Sus scrofa
J. Biol. Chem.
201
847-856
1953
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
29
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
29
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288852
Birnbaum
Specificity of amino acid acyl ...
Sus scrofa
J. Biol. Chem.
194
455-470
1952
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
42
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
42
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-