Any feedback?
Literature summary for 3.5.1.14 extracted from
- Preparation and properties of immobilized pig kidney aminoacylase and optical resolution of N-acyl-DL-alanine (1999), Appl. Biochem. Biotechnol., 76, 183-191.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | - |
Sus scrofa |
| synthesis | - |
Aspergillus oryzae |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Aspergillus oryzae |  |
| Co2+ | activates free and immobilized enzyme | Sus scrofa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-L-methionine | Sus scrofa | - |
L-methionine + acetate | - |
? | |
| N-acetyl-L-methionine | Aspergillus oryzae | - |
L-methionine + acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | - |
- |
- |
| Sus scrofa | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Sus scrofa | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, immobilized enzyme stable for more than 2 months without significant loss of activity | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-DL-methionine + H2O | - |
Sus scrofa | DL-methionine + acetate | - |
? | |
| N-acetyl-DL-methionine + H2O | - |
Aspergillus oryzae | DL-methionine + acetate | - |
? | |
| N-acetyl-L-alanine + H2O | - |
Sus scrofa | L-alanine + acetate | - |
? | |
| N-acetyl-L-alanine + H2O | - |
Aspergillus oryzae | L-alanine + acetate | - |
? | |
| N-acetyl-L-methionine | - |
Sus scrofa | L-methionine + acetate | - |
? | |
| N-acetyl-L-methionine | - |
Aspergillus oryzae | L-methionine + acetate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
immobilized enzyme | Sus scrofa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
immobilized enzyme more thermally stable, preserved during prolonged incubation at 70°C, soluble enzyme completely inactivated in 10 min under same conditions | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
immobilized enzyme | Sus scrofa |
| 8 | - |
- |
Sus scrofa |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
