Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.1.14 extracted from

  • Yang, Y.B.; Hu, H.L.; Chang, M.C.; Li, H.; Tsai, Y.C.
    Purification and characterization of L-aminoacylase from Alcaligenes denitrificans DA181 (1994), Biosci. Biotechnol. Biochem., 58, 204-205.
    View publication on PubMed
Search for more literature for 3.5.1.14

Application

Application Comment Organism
synthesis
-
 Sus scrofa
synthesis
-
 Bacillus sp. (in: Bacteria)
synthesis
-
 Aspergillus oryzae

Inhibitors

Inhibitors Comment Organism Structure
Cd2+
-
 Alcaligenes sp.
Cd2+
-
 Aspergillus oryzae
Cd2+
-
 Bacillus sp. (in: Bacteria)
Cu2+
-
 Alcaligenes sp.
Cu2+
-
 Aspergillus oryzae
Cu2+
-
 Bacillus sp. (in: Bacteria)
EDTA
-
 Alcaligenes sp.
EDTA
-
 Aspergillus oryzae
Pb2+
-
 Alcaligenes sp.
Zn2+
-
 Alcaligenes sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Alcaligenes sp. 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ enzyme strongly activated by Co2+ ions Sus scrofa
Co2+ enzyme strongly activated by Co2+ ions Bacillus sp. (in: Bacteria)
Co2+ enzyme strongly activated by Co2+ ions Alcaligenes sp.
Co2+ enzyme strongly activated by Co2+ ions Aspergillus oryzae
Zn2+ enzyme contains Zn as prosthetic metal Sus scrofa
Zn2+ enzyme contains Zn as prosthetic metal Bacillus sp. (in: Bacteria)
Zn2+ enzyme contains Zn as prosthetic metal Alcaligenes sp.
Zn2+ enzyme contains Zn as prosthetic metal Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 2 * 40000, SDS-PAGE Alcaligenes sp.
74000
-
-
 Aspergillus oryzae
80000
-
 gel filtration Alcaligenes sp.
175000
-
-
 Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-L-methionine Sus scrofa
-
 L-methionine + acetate
-
 ?
N-acetyl-L-methionine Bacillus sp. (in: Bacteria)
-
 L-methionine + acetate
-
 ?
N-acetyl-L-methionine Alcaligenes sp.
-
 L-methionine + acetate
-
 ?
N-acetyl-L-methionine Aspergillus oryzae
-
 L-methionine + acetate
-
 ?
N-acetyl-L-methionine Aspergillus oryzae No.9
-
 L-methionine + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Alcaligenes sp.
-
 denitrificans DA181, M14
-
Alcaligenes sp.
-
 xylosoxydans subsp. xylosoxydans A-6
-
Aspergillus oryzae
-
 No.9
-
Aspergillus oryzae No.9
-
 No.9
-
Bacillus sp. (in: Bacteria)
-
 thermoglucosidicus, strain DSM 2542
-
Cercidium texanum
-
 paloverde tree
-
Sus scrofa
-
 pig
-

Purification (Commentary)

Purification (Comment) Organism
-
 Alcaligenes sp.

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Cercidium texanum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-DL-methionine + H2O
-
 Sus scrofa DL-methionine + acetate
-
 ?
N-acetyl-DL-methionine + H2O
-
 Bacillus sp. (in: Bacteria) DL-methionine + acetate
-
 ?
N-acetyl-DL-methionine + H2O
-
 Alcaligenes sp. DL-methionine + acetate
-
 ?
N-acetyl-DL-methionine + H2O
-
 Aspergillus oryzae DL-methionine + acetate
-
 ?
N-acetyl-DL-methionine + H2O
-
 Aspergillus oryzae No.9 DL-methionine + acetate
-
 ?
N-acetyl-L-alanine + H2O
-
 Sus scrofa L-alanine + acetate
-
 ?
N-acetyl-L-alanine + H2O
-
 Bacillus sp. (in: Bacteria) L-alanine + acetate
-
 ?
N-acetyl-L-alanine + H2O
-
 Alcaligenes sp. L-alanine + acetate
-
 ?
N-acetyl-L-alanine + H2O
-
 Aspergillus oryzae L-alanine + acetate
-
 ?
N-acetyl-L-alanine + H2O
-
 Aspergillus oryzae No.9 L-alanine + acetate
-
 ?
N-acetyl-L-methionine
-
 Sus scrofa L-methionine + acetate
-
 ?
N-acetyl-L-methionine
-
 Bacillus sp. (in: Bacteria) L-methionine + acetate
-
 ?
N-acetyl-L-methionine
-
 Alcaligenes sp. L-methionine + acetate
-
 ?
N-acetyl-L-methionine
-
 Aspergillus oryzae L-methionine + acetate
-
 ?
N-acetyl-L-methionine
-
 Aspergillus oryzae No.9 L-methionine + acetate
-
 ?
N-acetyl-L-valine + H2O
-
 Sus scrofa L-valine + acetate
-
 ?
N-acetyl-L-valine + H2O
-
 Bacillus sp. (in: Bacteria) L-valine + acetate
-
 ?
N-acetyl-L-valine + H2O favorite substrate Alcaligenes sp. L-valine + acetate
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Sus scrofa
dimer
-
 Aspergillus oryzae
dimer 2 * 40000, SDS-PAGE Alcaligenes sp.
tetramer
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Sus scrofa
65
-
-
 Alcaligenes sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
-
 Sus scrofa
60
-
-
 Aspergillus oryzae
60
-
 retains 80% activity after 1 h at 60°C Alcaligenes sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Sus scrofa
8
-
-
 Alcaligenes sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 6.5
-
 Alcaligenes sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9
-
 Alcaligenes sp.