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Literature summary for 3.5.1.14 extracted from

  • Cho, H.Y.; Tanizawa, K.; Tanaka, H.; Soda, K.
    Thermostable aminoacylase from Bacillus thermoglucosidius: Purification and characterization (1987), Agric. Biol. Chem., 51, 2793-2800.
No PubMed abstract available
Search for more literature for 3.5.1.14

Application

Application Comment Organism
synthesis
-
 Sus scrofa
synthesis enzyme used for production of L-amino acids Aspergillus oryzae
synthesis thermostable enzyme useful for industrial production, optical resolution of DL-amino acids on an industrial scale, utilization in industrial production of stereoisomers from racemates Bacillus sp. (in: Bacteria)

General Stability

General Stability Organism
very active and physically stable, resistant to urea, 85% activity retained with 8 M urea at 37°C for 30 min Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
Cd2+
-
 Aspergillus oryzae
Cd2+
-
 Bacillus sp. (in: Bacteria)
Cu2+
-
 Aspergillus oryzae
Cu2+
-
 Bacillus sp. (in: Bacteria)
Fe2+
-
 Bacillus sp. (in: Bacteria)
Hg2+
-
 Bacillus sp. (in: Bacteria)
Ni2+
-
 Aspergillus oryzae
Ni2+
-
 Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
 N-acetyl-L-methionine addition of 0.2 mM Co2+ Bacillus sp. (in: Bacteria)
0.056
-
 N-acetyl-L-methionine addition of 0.2 mM Mn2+ Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ enzyme strongly activated by Co2+ ions Sus scrofa
Co2+ enzyme strongly activated by Co2+ ions Bacillus sp. (in: Bacteria)
Co2+ enzyme strongly activated by Co2+ ions Aspergillus oryzae
Mn2+
-
 Bacillus sp. (in: Bacteria)
Zn2+ enzyme contains Zn as prosthetic metal Sus scrofa
Zn2+ enzyme contains Zn as prosthetic metal Bacillus sp. (in: Bacteria)
Zn2+ enzyme contains Zn as prosthetic metal Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
-
 Aspergillus oryzae
37000
-
 subunit, SDS-PAGE Bacillus sp. (in: Bacteria)
42500
-
 calculated from amino acid composition Bacillus sp. (in: Bacteria)
43000
-
 2 * 43000, SDS-PAGE Sus scrofa
43000
-
 2 * 43000, SDS-PAGE Aspergillus oryzae
43000
-
 4 * 43000, 4gatom Zn per mol enzyme, SDS-PAGE Bacillus sp. (in: Bacteria)
175000
-
 gel permeation Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-L-methionine Sus scrofa
-
 L-methionine + acetate
-
 ?
N-acetyl-L-methionine Bacillus sp. (in: Bacteria)
-
 L-methionine + acetate
-
 ?
N-acetyl-L-methionine Aspergillus oryzae
-
 L-methionine + acetate
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-
Bacillus sp. (in: Bacteria)
-
 thermoglucosidicus, strain DSM 2542
-
Sus scrofa
-
 pig
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sus scrofa
-
 Bacillus sp. (in: Bacteria)
-
 Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-DL-methionine + H2O
-
 Sus scrofa DL-methionine + acetate
-
 ?
N-acetyl-DL-methionine + H2O
-
 Bacillus sp. (in: Bacteria) DL-methionine + acetate
-
 ?
N-acetyl-DL-methionine + H2O
-
 Aspergillus oryzae DL-methionine + acetate
-
 ?
N-acetyl-DL-norleucine + H2O
-
 Sus scrofa DL-norleucine + acetate
-
 ?
N-acetyl-DL-norleucine + H2O
-
 Bacillus sp. (in: Bacteria) DL-norleucine + acetate
-
 ?
N-acetyl-L-alanine + H2O
-
 Sus scrofa L-alanine + acetate
-
 ?
N-acetyl-L-alanine + H2O
-
 Bacillus sp. (in: Bacteria) L-alanine + acetate
-
 ?
N-acetyl-L-alanine + H2O
-
 Aspergillus oryzae L-alanine + acetate
-
 ?
N-acetyl-L-glutamine + H2O
-
 Sus scrofa L-glutamine + acetate
-
 ?
N-acetyl-L-glutamine + H2O
-
 Bacillus sp. (in: Bacteria) L-glutamine + acetate
-
 ?
N-acetyl-L-glutamine + H2O
-
 Aspergillus oryzae L-glutamine + acetate
-
 ?
N-acetyl-L-methionine
-
 Sus scrofa L-methionine + acetate
-
 ?
N-acetyl-L-methionine
-
 Bacillus sp. (in: Bacteria) L-methionine + acetate
-
 ?
N-acetyl-L-methionine
-
 Aspergillus oryzae L-methionine + acetate
-
 ?
N-acetyl-L-phenylalanine + H2O
-
 Sus scrofa L-phenylalanine + acetate
-
 ?
N-acetyl-L-phenylalanine + H2O
-
 Bacillus sp. (in: Bacteria) L-phenylalanine + acetate
-
 ?
N-acetyl-L-valine + H2O
-
 Sus scrofa L-valine + acetate
-
 ?
N-acetyl-L-valine + H2O
-
 Bacillus sp. (in: Bacteria) L-valine + acetate
-
 ?
N-chloroacetyl-DL-valine + H2O
-
 Sus scrofa chloroacetate + DL-valine
-
 ?
N-chloroacetyl-DL-valine + H2O
-
 Bacillus sp. (in: Bacteria) chloroacetate + DL-valine
-
 ?
N-chloroacetyl-L-leucine + H2O
-
 Sus scrofa chloroacetate + L-leucine
-
 ?
N-chloroacetyl-L-leucine + H2O
-
 Bacillus sp. (in: Bacteria) chloroacetate + L-leucine
-
 ?
N-chloroacetyl-L-leucine + H2O
-
 Aspergillus oryzae chloroacetate + L-leucine
-
 ?
N-chloroacetylglycine + H2O N-chloroacetylamino acids are better substrates than N-acetylamino acids Bacillus sp. (in: Bacteria) chloroacetate + glycine
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 43000, SDS-PAGE Sus scrofa
dimer 2 * 43000, SDS-PAGE Aspergillus oryzae
tetramer 4 * 43000, 4gatom Zn per mol enzyme, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70 80 retains full activity on heating at 70°C for 10 min, 90% of the original activity after heating at 80%, much activity lost on 30 min at 80°C Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
 Sus scrofa
8 8.5
-
 Aspergillus oryzae
8.3
-
-
 Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 11.5 no activity lost at 37°C for 1h Bacillus sp. (in: Bacteria)