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Literature summary for 3.5.1.137 extracted from
- Isolation of a constitutively expressed enzyme for hydrolysis of carbaryl in Pseudomonas aeruginosa (1993), J. Bacteriol., 175, 6711-6716 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.009 | - |
Carbaryl | at pH 7.0 and 30°C | Pseudomonas aeruginosa |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Pseudomonas aeruginosa | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Ba2+, Ca2+, Co2+, Cu2+, Fe2+, Hg2+, Li2+, Mg2+, Mn2+, and Sr2+ do not have any effect on the activity of the enzyme | Pseudomonas aeruginosa |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
gel filtration | Pseudomonas aeruginosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas aeruginosa | - |
isolate 50581 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-cellulose column chromatography, hydroxylapatite column chromatography, and Sephacryl S-300 gel filtration | Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.68 | - |
crude extract, at pH 7.0 and 30°C | Pseudomonas aeruginosa |
| 1201.8 | - |
after 1767fold purification, at pH 7.0 and 30°C | Pseudomonas aeruginosa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, purified enzyme in the presence of 20% (v/v) glycerol, 1 month, no loss of activity | Pseudomonas aeruginosa |
| 25°C, purified enzyme, 10 days, 10% loss of activity | Pseudomonas aeruginosa |
| 25°C, purified enzyme, 12 h, 15% loss of activity | Pseudomonas aeruginosa |
| 25°C, purified enzyme, 25 days, 47% loss of activity | Pseudomonas aeruginosa |
| 25°C, purified enzyme, 7 days, no loss of activity | Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carbaryl + H2O | - |
Pseudomonas aeruginosa | 1-naphthol + methylamine + CO2 | - |
? | |
| additional information | no activity with carbofuran and aldicarb | Pseudomonas aeruginosa | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 65000, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| carbaryl hydrolase | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Pseudomonas aeruginosa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
the enzyme loses its catalytic activity when heated to 65°C for 5 min | Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Pseudomonas aeruginosa |
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Release 2023.1 (January 2023)
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