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Literature summary for 3.5.1.137 extracted from

  • Karns, J.; Tomasek, P.
    Carbofuran hydrolase - Purification and properties (1991), J. Agric. Food Chem., 39, 1004-1008 .
No PubMed abstract available
Search for more literature for 3.5.1.137

Inhibitors

Inhibitors Comment Organism Structure
EDTA the addition of EDTA results in the complete inactivation of the enzyme. Addition of 1 mM Mn2+ to the reaction mixture restores the activity to 70% of its original level Achromobacter sp. WM111

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.063
-
 carbofuran at pH 8.5 and 37°C Achromobacter sp. WM111
2.7
-
 2-nitrophenyl dimethylcarbamate at pH 8.5 and 37°C Achromobacter sp. WM111

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required for enzyme activity. The enzyme preparation is stabilized at all stages of the purification regimen by manganese ions Achromobacter sp. WM111
additional information not influenced by Mg2+, Ca2+, Co2+, Cu2+, Fe2+, Ni2+, Pd2+, Sn2+, or Zn2+ Achromobacter sp. WM111

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
 density gradient centrifugation Achromobacter sp. WM111
170000
-
 gel filtration Achromobacter sp. WM111

Organism

Organism UniProt Comment Textmining
Achromobacter sp. WM111
-
-
-

Purification (Commentary)

Purification (Comment) Organism
TSK-DEAE column chromatography, TSK-phenyl column chromatography, and Bio-Gel A0.5m gel filtration Achromobacter sp. WM111

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.14
-
 crude enzyme from supernatant, at pH 8.5 and 37°C Achromobacter sp. WM111
7.85
-
 after 58.1fold purification, at pH 8.5 and 37°C Achromobacter sp. WM111

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl dimethylcarbamate + H2O
-
 Achromobacter sp. WM111 ? + methylamine + CO2
-
 ?
aldicarb + H2O
-
 Achromobacter sp. WM111 ? + methylamine + CO2
-
 ?
carbaryl + H2O
-
 Achromobacter sp. WM111 1-naphthol + methylamine + CO2
-
 ?
carbofuran + H2O
-
 Achromobacter sp. WM111 ? + methylamine + CO2
-
 ?
additional information the purified enzyme has no urease activity Achromobacter sp. WM111 ?
-
-

Synonyms

Synonyms Comment Organism
carbofuran hydrolase
-
 Achromobacter sp. WM111

pI Value

Organism Comment pI Value Maximum pI Value
Achromobacter sp. WM111 isoelectric focusing
-
 4.8