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Literature summary for 3.5.1.137 extracted from

  • Hayatsu, M.; Mizutani, A.; Hashimoto, M.; Sato, K.; Hayano, K.
    Purification and characterization of carbaryl hydrolase from Arthrobacter sp. RC100 (2001), FEMS Microbiol. Lett., 201, 99-103 .
    View publication on PubMed
Search for more literature for 3.5.1.137

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 110% activity at 1 mM Arthrobacter sp. RC100

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 90% residual activity at 1 mM Arthrobacter sp. RC100
diisopropylfluorophosphate 37% residual activity at 1 mM Arthrobacter sp. RC100
Hg2+ 20% residual activity at 1 mM Arthrobacter sp. RC100
additional information not inhibited by EDTA, N-ethylmaleimide and iodoacetamide Arthrobacter sp. RC100
paraoxon less than 1% residual activity at 1 mM Arthrobacter sp. RC100
phenylmethylsulfonyl fluoride less than 4% residual activity at 1 mM Arthrobacter sp. RC100

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 gel filtration Arthrobacter sp. RC100

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an N-methyl carbamate ester + H2O Arthrobacter sp. RC100
-
 an alcohol + methylamine + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Arthrobacter sp. RC100
-
-
-

Purification (Commentary)

Purification (Comment) Organism
protamine sulfate treatment, ammonium sulfate precipitation, phenyl Sepharose column chromatography, DEAE-Sepharose column chromatography, and Superdex 200 gel filtration Arthrobacter sp. RC100

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.043
-
 crude extract, at pH 7.0 and 30°C Arthrobacter sp. RC100
0.388
-
 after 9.1fold purification, at pH 7.0 and 30°C Arthrobacter sp. RC100

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl acetate + H2O 51% activity compared to carbaryl Arthrobacter sp. RC100 1-naphthol + acetate
-
 ?
3,5-dimethylphenyl N-methylcarbamate + H2O 43% activity compared to carbaryl Arthrobacter sp. RC100 ? + methylamine + CO2
-
 ?
4-nitrophenyl acetate + H2O 80% activity compared to carbaryl Arthrobacter sp. RC100 4-nitrophenol + acetate
-
 ?
an N-methyl carbamate ester + H2O
-
 Arthrobacter sp. RC100 an alcohol + methylamine + CO2
-
 ?
carbaryl + H2O 100% activity Arthrobacter sp. RC100 1-naphthol + methylamine + CO2
-
 ?
metolcarb + H2O 304% activity compared to carbaryl Arthrobacter sp. RC100 ? + methylamine + CO2
-
 ?
additional information the enzyme is not able to hydrolyze chloropropham, fenobucarb, propoxur, and isoprocarb Arthrobacter sp. RC100 ?
-
-
xylylcarb + H2O 66% activity compared to carbaryl Arthrobacter sp. RC100 ? + methylamine + CO2
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 51000, SDS-PAGE Arthrobacter sp. RC100

Synonyms

Synonyms Comment Organism
carbaryl hydrolase
-
 Arthrobacter sp. RC100

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Arthrobacter sp. RC100

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 70 40% of the initial activity is retained after 60 min at 60°C. The enzyme is completely inactivated by heating for 60 min at 70°C Arthrobacter sp. RC100

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Arthrobacter sp. RC100

pH Range

pH Minimum pH Maximum Comment Organism
6 10 the enzyme retains over 80% of its maximum activity between pH 6.0 and 10.0 Arthrobacter sp. RC100