Literature summary for 3.5.1.137 extracted from

Jiang, W.; Gao, Q.; Zhang, L.; Wang, H.; Zhang, M.; Liu, X.; Zhou, Y.; Ke, Z.; Wu, C.; Qiu, J.; Hong, Q.
Identification of the key amino acid sites of the carbofuran hydrolase CehA from a newly isolated carbofuran-degrading strain Sphingbium sp. CFD-1 (2020), Ecotoxicol. Environ. Saf., 189, 109938 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Sphingobium sp. CFD-1

Protein Variants

Protein Variants	Comment	Organism
H351A	the mutant loses hydrolysis activity against carbofuran	Sphingobium sp. CFD-1
H353A	the mutant loses hydrolysis activity against carbofuran	Sphingobium sp. CFD-1
H453A	the mutant loses hydrolysis activity against carbofuran	Sphingobium sp. CFD-1
H495A	the mutant loses hydrolysis activity against carbofuran	Sphingobium sp. CFD-1

Inhibitors

Inhibitors	Comment	Organism	Structure
Fe3+	-	Sphingobium sp. CFD-1
Hg2+	-	Sphingobium sp. CFD-1

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.13322	-	carbofuran	at pH 7.0 and 30Â°C	Sphingobium sp. CFD-1

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	activates	Sphingobium sp. CFD-1
Cu2+	activates	Sphingobium sp. CFD-1
additional information	Fe2+, Ca2+, Mn2+ and Mg2+ have no obvious effects on the enzyme activity	Sphingobium sp. CFD-1
Ni2+	activates	Sphingobium sp. CFD-1
Zn2+	activates	Sphingobium sp. CFD-1

Organism

Organism	UniProt	Comment	Textmining
Sphingobium sp. CFD-1	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose resin column chromatography and Superdex 200 gel filtration	Sphingobium sp. CFD-1

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
aldicarb + H2O	-	Sphingobium sp. CFD-1	? + methylamine + CO2	-	?
carbaryl + H2O	-	Sphingobium sp. CFD-1	1-naphthol + methylamine + CO2	-	?
carbofuran + H2O	-	Sphingobium sp. CFD-1	carbofuran phenol + methylamine + CO2	-	?
isoprocarb + H2O	-	Sphingobium sp. CFD-1	? + methylamine + CO2	-	?
additional information	no activity with methomyl	Sphingobium sp. CFD-1	?	-	-
oxamyl + H2O	-	Sphingobium sp. CFD-1	? + methylamine + CO2	-	?
propoxur + H2O	-	Sphingobium sp. CFD-1	? + methylamine + CO2	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 87660, calculated from amino acid sequence	Sphingobium sp. CFD-1

Synonyms

Synonyms	Comment	Organism
carbofuran hydrolase	-	Sphingobium sp. CFD-1
cehA	-	Sphingobium sp. CFD-1

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Sphingobium sp. CFD-1

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
9.48	-	carbofuran	at pH 7.0 and 30Â°C	Sphingobium sp. CFD-1

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Sphingobium sp. CFD-1

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Release 2023.1 (January 2023)