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Literature summary for 3.5.1.137 extracted from

  • Kamini, Q.; Shetty, D.; Trivedi, V.D.; Varunjikar, M.; Phale, P.S.
    Compartmentalization of the carbaryl degradation pathway Molecular characterization of inducible periplasmic carbaryl hydrolase from Pseudomonas spp. (2018), Appl. Environ. Microbiol., 84, e02115-17 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.137

Cloned(Commentary)

Cloned (Comment) Organism
enzyme variant rCHDELTA(Tmd)a is expressed in Escherichia coli BL21(DE3) cells Pseudomonas sp. C5pp

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.098
-
 Carbaryl native enzyme, at pH 7.5, temperature not specified in the publication Pseudomonas sp. C5pp
0.111
-
 Carbaryl native enzyme, at pH 7.5, temperature not specified in the publication Pseudomonas sp. C7
0.111
-
 Carbaryl recombinant enzyme rCHDELTA(Tmd)a, at pH 7.5, temperature not specified in the publication Pseudomonas sp. C5pp

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Pseudomonas sp. C5pp
-
-
periplasm
-
 Pseudomonas sp. C7
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 native enzyme, gel filtration Pseudomonas sp. C5pp
70000
-
 native enzyme, gel filtration Pseudomonas sp. C7

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
carbaryl + H2O Pseudomonas sp. C5pp
-
 1-naphthol + methylamine + CO2
-
 ?
carbaryl + H2O Pseudomonas sp. C7
-
 1-naphthol + methylamine + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp. C5pp A0A140DCH5
-
-
Pseudomonas sp. C7
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography, Sepharose CL-4B column chromatography, hydroxyapatite column chromatography and Sephacryl S-200HR gel filtration Pseudomonas sp. C7
Q-Sepharose column chromatography, Sepharose CL-4B column chromatography, hydroxyapatite column chromatography and Sephacryl S-200HR gel filtration, and Ni-NTA column chromatography Pseudomonas sp. C5pp

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbaryl + H2O
-
 Pseudomonas sp. C5pp 1-naphthol + methylamine + CO2
-
 ?
carbaryl + H2O
-
 Pseudomonas sp. C7 1-naphthol + methylamine + CO2
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 68000, native enzyme, SDS-PAGE Pseudomonas sp. C5pp
monomer 1 * 68000, native enzyme, SDS-PAGE Pseudomonas sp. C7
monomer 1 * 76000, recombinant enzyme rCHDELTA(Tmd)a, SDS-PAGE Pseudomonas sp. C5pp

Synonyms

Synonyms Comment Organism
carbaryl hydrolase
-
 Pseudomonas sp. C5pp
carbaryl hydrolase
-
 Pseudomonas sp. C7
mcbA
-
 Pseudomonas sp. C5pp
mcbA
-
 Pseudomonas sp. C7

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
81
-
 Carbaryl native enzyme, at pH 7.5, temperature not specified in the publication Pseudomonas sp. C5pp
84
-
 Carbaryl native enzyme, at pH 7.5, temperature not specified in the publication Pseudomonas sp. C7
86
-
 Carbaryl recombinant enzyme rCHDELTA(Tmd)a, at pH 7.5, temperature not specified in the publication Pseudomonas sp. C5pp

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
760
-
 Carbaryl native enzyme, at pH 7.5, temperature not specified in the publication Pseudomonas sp. C7
770
-
 Carbaryl recombinant enzyme rCHDELTA(Tmd)a, at pH 7.5, temperature not specified in the publication Pseudomonas sp. C5pp
830
-
 Carbaryl native enzyme, at pH 7.5, temperature not specified in the publication Pseudomonas sp. C5pp