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Literature summary for 3.5.1.137 extracted from

  • Hayatsu, M.; Nagata, T.
    Purification and characterization of carbaryl hydrolase from Blastobacter sp. strain M501 (1993), Appl. Environ. Microbiol., 59, 2121-2125 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.137

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ complete inhibition at 1 mM Blastobacter sp. M501
additional information EDTA, iodoacetoamide, diisopropyl fluorophosphate, and paraoxon have no effect on the enzyme activity Blastobacter sp. M501

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.055
-
 Carbaryl at pH 7.0 and 30°C Blastobacter sp. M501
0.172
-
 xylylcarb at pH 7.0 and 30°C Blastobacter sp. M501

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Fe2+, Fe3+, Cu2+, Zn2+, Ba2+, Ca2+, Co2+, K+, Mg2+, Mn2+, and Mn3+ do not affect the enzyme activity Blastobacter sp. M501

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
166000
-
 gel filtration Blastobacter sp. M501

Organism

Organism UniProt Comment Textmining
Blastobacter sp. M501
-
-
-

Purification (Commentary)

Purification (Comment) Organism
protamine sulfate treatment, ammonium sulfate precipitation, Phenyl-Sepharose CL-4B column chromatography, DEAE-Toyopearl 650 column chromatography, Toyopearl HW-55 gel filtration, and hydroxylapatite column chromatography Blastobacter sp. M501

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3
-
 crude extract, at pH 7.0 and 30°C Blastobacter sp. M501
704.3
-
 after 306.2fold purification, at pH 7.0 and 30°C Blastobacter sp. M501

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl acetate + H2O 2% activity compared to carbaryl Blastobacter sp. M501 1-naphthol + acetate
-
 ?
3,5-dimethylphenyl N-methylcarbamate + H2O 66% activity compared to carbaryl Blastobacter sp. M501 ?
-
 ?
4-nitrophenyl acetate + H2O 4% activity compared to carbaryl Blastobacter sp. M501 4-nitrophenol + acetate
-
 ?
carbaryl + H2O i.e. 1-naphthyl N-methylcarbamate, 100% activity Blastobacter sp. M501 1-naphthol + methylamine + CO2
-
 ?
fenobucarb + H2O i.e 2-S-butylphenyl N-methylcarbamate, 2% activity compared to carbaryl Blastobacter sp. M501 ?
-
 ?
isoprocarb + H2O i.e. 2-isopropylphenyl N-methylcarbamate, 2% activity compared to carbaryl Blastobacter sp. M501 ?
-
 ?
metolcarb + H2O i.e. 3-methylphenyl N-methylcarbamate, 62% activity compared to carbaryl Blastobacter sp. M501 ?
-
 ?
additional information hydrolase activity is not detected with the substrates of fenitrothion, methylparathion, paraoxon, chloropropham, swep, propanil, and linuron Blastobacter sp. M501 ?
-
-
propoxur + H2O i.e. 2-isopropoxyphenyl N-methylcarbamate, 14% activity compared to carbaryl Blastobacter sp. M501 ?
-
 ?
xylylcarb + H2O i.e. 3,4-dimethylphenyl N-methylcarbamate, 88% activity compared to carbaryl Blastobacter sp. M501 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 84000, SDS-PAGE Blastobacter sp. M501

Synonyms

Synonyms Comment Organism
carbaryl hydrolase
-
 Blastobacter sp. M501

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Blastobacter sp. M501

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 50 the enzyme is stable at temperature up to 30°C, and 50% of the initial activity is retained at 50°C. The enzyme is not stable at temperatures above 40°C Blastobacter sp. M501

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 at 30°C Blastobacter sp. M501

pH Range

pH Minimum pH Maximum Comment Organism
6 11 the enzyme retains over 80% of its maximum activity between pH values of 6.0 to 11.0 Blastobacter sp. M501

pH Stability

pH Stability pH Stability Maximum Comment Organism
10 11 the hydrolase is stable in a pH range of 10.0 to 11.0 Blastobacter sp. M501