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Literature summary for 3.5.1.136 extracted from
- Concerted action of diacetylchitobiose deacetylase and exo-beta-D-glucosaminidase in a novel chitinolytic pathway in the hyperthermophilic archaeon Thermococcus kodakaraensis KOD1 (2004), J. Biol. Chem., 279, 30021-30027 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Thermococcus kodakarensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 31.8 | - |
N,N'-diacetylchitobiose | pH 8.0, 70°C | Thermococcus kodakarensis |  |
| 98 | - |
N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine | pH 8.0, 70°C | Thermococcus kodakarensis | |
| 127 | - |
N-acetyl-D-glucosamine | pH 8.0, 70°C | Thermococcus kodakarensis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Thermococcus kodakarensis | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 160000 | - |
gel filtration | Thermococcus kodakarensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N,N'-diacetylchitobiose + H2O | Thermococcus kodakarensis | the enzyme is involved in the chitin catabolic pathway | beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermococcus kodakarensis | Q6F4N1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Thermococcus kodakarensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N,N'-diacetylchitobiose + H2O | - |
Thermococcus kodakarensis | beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate | - |
? | |
| N,N'-diacetylchitobiose + H2O | the enzyme is involved in the chitin catabolic pathway | Thermococcus kodakarensis | beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate | - |
? | |
| N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + H2O | - |
Thermococcus kodakarensis | beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate | - |
? | |
| N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + H2O | - |
Thermococcus kodakarensis | beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate | - |
? | |
| N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + H2O | - |
Thermococcus kodakarensis | beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate | - |
? | |
| N-acetyl-D-glucosamine + H2O | - |
Thermococcus kodakarensis | beta-D-glucosamine + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homohexamer | 6 * 30000, SDS-PAGE | Thermococcus kodakarensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NAc2 deacetylase | - |
Thermococcus kodakarensis |
| Tk-Dac | - |
Thermococcus kodakarensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Thermococcus kodakarensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 100 | 37°C: about 20% of maximal activity, 100°C: about 20% of maximal activity | Thermococcus kodakarensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Thermococcus kodakarensis |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Thermococcus kodakarensis | expression is not observed under basal culture condition. It is induced by the addition of GlcNAc2. GlcNAc, lactose, cellobiose, maltose or GlcN can not act as an inducer | up |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0684 | - |
N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine | pH 8.0, 70°C | Thermococcus kodakarensis | |
| 0.529 | - |
N-acetyl-D-glucosamine | pH 8.0, 70°C | Thermococcus kodakarensis | |
| 0.77 | - |
N,N'-diacetylchitobiose | pH 8.0, 70°C | Thermococcus kodakarensis | |
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