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Literature summary for 3.5.1.136 extracted from

  • Jiang, Z.; Niu, T.; Lv, X.; Liu, Y.; Li, J.; Lu, W.; Du, G.; Chen, J.; Liu, L.
    Secretory expression fine-tuning and directed evolution of diacetylchitobiose deacetylase by Bacillus subtilis (2019), Appl. Environ. Microbiol., 85, e01076-19 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis production of chitosan oligosaccharides and monosaccharides Pyrococcus horikoshii

Cloned(Commentary)

Cloned (Comment) Organism
expression in Bacillus subtilis Pyrococcus horikoshii

Protein Variants

Protein Variants Comment Organism
R157T mutant enzyme exhibits much higher specific activity and catalytic efficiency as compared to wild-type enzyme Pyrococcus horikoshii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.19
-
N,N'-diacetylchitobiose pH 8.0, 40°C, mutant enzyme R157T Pyrococcus horikoshii
7.04
-
N,N'-diacetylchitobiose pH 8.0, 40°C, wild-type enzyme Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N,N'-diacetylchitobiose + H2O Pyrococcus horikoshii
-
beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
?
N,N'-diacetylchitobiose + H2O Pyrococcus horikoshii DSM 12428
-
beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
?
N,N'-diacetylchitobiose + H2O Pyrococcus horikoshii NBRC 100139
-
beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
?
N,N'-diacetylchitobiose + H2O Pyrococcus horikoshii JCM 9974
-
beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
?
N,N'-diacetylchitobiose + H2O Pyrococcus horikoshii ATCC 700860
-
beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
?
N,N'-diacetylchitobiose + H2O Pyrococcus horikoshii OT-3
-
beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
?

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O58235
-
-
Pyrococcus horikoshii ATCC 700860 O58235
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N,N'-diacetylchitobiose + H2O
-
Pyrococcus horikoshii beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
?
N,N'-diacetylchitobiose + H2O
-
Pyrococcus horikoshii DSM 12428 beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
?
N,N'-diacetylchitobiose + H2O
-
Pyrococcus horikoshii NBRC 100139 beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
?
N,N'-diacetylchitobiose + H2O
-
Pyrococcus horikoshii JCM 9974 beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
?
N,N'-diacetylchitobiose + H2O
-
Pyrococcus horikoshii ATCC 700860 beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
?
N,N'-diacetylchitobiose + H2O
-
Pyrococcus horikoshii OT-3 beta-D-glucosaminyl-(1->4)-N-acetyl-D-glucosamine + acetate
-
?

Synonyms

Synonyms Comment Organism
PH0499
-
Pyrococcus horikoshii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
assay at Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Pyrococcus horikoshii