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Literature summary for 3.5.1.130 extracted from

  • Horie, A.; Tomita, T.; Saiki, A.; Kono, H.; Taka, H.; Mineki, R.; Fujimura, T.; Nishiyama, C.; Kuzuyama, T.; Nishiyama, M.
    Discovery of proteinaceous N-modification in lysine biosynthesis of Thermus thermophilus (2009), Nat. Chem. Biol., 5, 673-679 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermus thermophilus

Inhibitors

Inhibitors Comment Organism Structure
[carrier protein LysW]-C-terminal-alpha-(L-lysyl)-L-glutamate
-
Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Thermus thermophilus for the conversion of alpha-aminoadipate to lysine, the amino group of alpha-aminoadipate is modified by attachment to the gamma-carboxyl group of the C-terminal Glu54 of a small protein, LysW. The side chain of alpha-aminoadipate is converted to the lysyl side chain while still attached to LysW, and lysine is subsequently liberated from the LysW-lysine fusion ?
-
?
additional information Thermus thermophilus DSM 7039 for the conversion of alpha-aminoadipate to lysine, the amino group of alpha-aminoadipate is modified by attachment to the gamma-carboxyl group of the C-terminal Glu54 of a small protein, LysW. The side chain of alpha-aminoadipate is converted to the lysyl side chain while still attached to LysW, and lysine is subsequently liberated from the LysW-lysine fusion ?
-
?
[carrier protein LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate + H2O Thermus thermophilus
-
[carrier protein LysW]-C-terminal-L-glutamate + L-lysine
-
?
[carrier protein LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate + H2O Thermus thermophilus DSM 7039
-
[carrier protein LysW]-C-terminal-L-glutamate + L-lysine
-
?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q8VUS5
-
-
Thermus thermophilus DSM 7039 Q8VUS5
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information for the conversion of alpha-aminoadipate to lysine, the amino group of alpha-aminoadipate is modified by attachment to the gamma-carboxyl group of the C-terminal Glu54 of a small protein, LysW. The side chain of alpha-aminoadipate is converted to the lysyl side chain while still attached to LysW, and lysine is subsequently liberated from the LysW-lysine fusion Thermus thermophilus ?
-
?
additional information no substrates: [carrier protein LysW]-C-terminal-alpha-(L-lysyl)-L-glutamate, [carrier protein LysW]-13-alpha-(L-lysyl)-L-glutamate Thermus thermophilus ?
-
?
additional information for the conversion of alpha-aminoadipate to lysine, the amino group of alpha-aminoadipate is modified by attachment to the gamma-carboxyl group of the C-terminal Glu54 of a small protein, LysW. The side chain of alpha-aminoadipate is converted to the lysyl side chain while still attached to LysW, and lysine is subsequently liberated from the LysW-lysine fusion Thermus thermophilus DSM 7039 ?
-
?
additional information no substrates: [carrier protein LysW]-C-terminal-alpha-(L-lysyl)-L-glutamate, [carrier protein LysW]-13-alpha-(L-lysyl)-L-glutamate Thermus thermophilus DSM 7039 ?
-
?
[carrier protein LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate + H2O
-
Thermus thermophilus [carrier protein LysW]-C-terminal-L-glutamate + L-lysine
-
?
[carrier protein LysW]-C-terminal-gamma-(L-lysyl)-L-glutamate + H2O
-
Thermus thermophilus DSM 7039 [carrier protein LysW]-C-terminal-L-glutamate + L-lysine
-
?

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00067
-
pH 8.0, 60°C Thermus thermophilus [carrier protein LysW]-C-terminal-alpha-(L-lysyl)-L-glutamate