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Literature summary for 3.5.1.13 extracted from

  • Rajesh, R.V.; Chitra, L.; Layer, P.G.; Boopathy, R.
    The aryl acylamidase activity is much more sensitive to Alzheimer drugs than the esterase activity of acetylcholinesterase in chicken embryonic brain (2009), Biochimie, 91, 1087-1094.
    View publication on PubMed
Search for more literature for 3.5.1.13

Inhibitors

Inhibitors Comment Organism Structure
1,5-bis(4-allyl-dimethylammonium-phenyl)pentan-3-one-dibromide i.e. BW284c51, inhibition is more pronounced at older developmental stages Gallus gallus
donepezil an Alzheimer drug Gallus gallus
huperzine an Alzheimer drug Gallus gallus
additional information the aryl acylamidase activity is associated with acetylcholinesterase in chicken embryonic brain, and is much more sensitive to Alzheimer drugs, huperzine and donepezil, especially at younger developmental stages, than the esterase activity of the acetylcholinesterase, whereas inhibition of esterase activity is sensitive to tacrine and DFP Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
 white Leghorn chicken
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from brain by ammonium sulfate fractionation and procainamide affinity chromatography Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
brain embryonic, the aryl acylamidase is associated with acetylcholinesterase in chicken embryonic brain Gallus gallus
-
additional information AAA activity shows a correlated expression with esterase activity at all stages, but the relative ratios of AAA to esterase activity are higher at younger stages, developmental expression patterns of AAA and AChE, overview Gallus gallus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitroacetanilide + H2O
-
 Gallus gallus 2-nitroaniline + acetate
-
 ?

Synonyms

Synonyms Comment Organism
AAA
-
 Gallus gallus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 50 assay at Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Gallus gallus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00001
-
 pH 7.0, 40-50°C Gallus gallus huperzine
0.0001
-
 pH 7.0, 40-50°C Gallus gallus donepezil
0.0001
-
 pH 7.0, 40-50°C Gallus gallus 1,5-bis(4-allyl-dimethylammonium-phenyl)pentan-3-one-dibromide