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Literature summary for 3.5.1.13 extracted from

  • Heumann, S.; Eberl, A.; Fischer-Colbrie, G.; Pobeheim, H.; Kaufmann, F.; Ribitsch, D.; Cavaco-Paulo, A.; Guebitz, G.M.
    A novel aryl acylamidase from Nocardia farcinica hydrolyses polyamide (2009), Biotechnol. Bioeng., 102, 1003-1011.
    View publication on PubMed
Search for more literature for 3.5.1.13

Inhibitors

Inhibitors Comment Organism Structure
1-aminohexane
-
 Nocardia farcinica
AgNO3
-
 Nocardia farcinica
CuSO4
-
 Nocardia farcinica
HgCl2
-
 Nocardia farcinica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 p-nitroacetanilide
-
 Nocardia farcinica
5.5
-
 hexanoamide
-
 Nocardia farcinica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 4 * 54000 Da, SDS-PAGE, 4 * 50000, calculated from tryptic digest/LC-MS/MS analysis Nocardia farcinica
54000
-
 4 * 54000 Da, SDS-PAGE, 4 * 50000, calculated from tryptic digest/LC-MS/MS analysis Nocardia farcinica
190000
-
 gel filtration Nocardia farcinica

Organism

Organism UniProt Comment Textmining
Nocardia farcinica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Nocardia farcinica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4
-
-
 Nocardia farcinica

Storage Stability

Storage Stability Organism
-18°C, 1 week, 18% loss of activity Nocardia farcinica
25°C, 1 week, 5% loss of activity Nocardia farcinica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetamide + H2O
-
 Nocardia farcinica acetic acid + NH3
-
 ?
adipic acid bishexylamide + H2O
-
 Nocardia farcinica adipic acid + dihexylamine
-
 ?
asparagine + H2O
-
 Nocardia farcinica aspartic acid + NH3
-
 ?
benzamide + H2O
-
 Nocardia farcinica benzoic acid + NH3
-
 ?
butyramide + H2O
-
 Nocardia farcinica butanoic acid + NH3
-
 ?
hexanoamide + ?
-
 Nocardia farcinica hexanoic acid + NH3
-
 ?
mandelamine + H2O
-
 Nocardia farcinica mandelic acid + NH3
-
 ?
methacrylamide + H2O
-
 Nocardia farcinica methacrylic acid + NH3
-
 ?
additional information surface hydrolysis of polyamide 6 Nocardia farcinica ?
-
 ?
nicotinamide + H2O
-
 Nocardia farcinica nicotinic acid + NH3
-
 ?
p-nitroacetanilide + H2O
-
 Nocardia farcinica 4-nitroaniline + acetic acid
-
 ?
propionamide + H2O
-
 Nocardia farcinica propanoic acid + NH3
-
 ?
stearamide + H2O
-
 Nocardia farcinica stearic acid + NH3
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 54000 Da, SDS-PAGE, 4 * 50000, calculated from tryptic digest/LC-MS/MS analysis Nocardia farcinica

Synonyms

Synonyms Comment Organism
aryl acylamidase
-
 Nocardia farcinica
polyamidase
-
 Nocardia farcinica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 pH8 Nocardia farcinica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 half life time: 35 min Nocardia farcinica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5
-
 hexanoamide
-
 Nocardia farcinica
5.8
-
 p-nitroacetanilide
-
 Nocardia farcinica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 11
-
 Nocardia farcinica

pH Range

pH Minimum pH Maximum Comment Organism
6 11
-
 Nocardia farcinica

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.2
-
 HgCl2
-
 Nocardia farcinica
0.2
-
 AgNO3
-
 Nocardia farcinica
2.7
-
 1-aminohexane
-
 Nocardia farcinica
3.2
-
 CuSO4
-
 Nocardia farcinica