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Literature summary for 3.5.1.13 extracted from
- Enzymatic hydrolysis of p-nitroacetanilide: mechanistic studies of the aryl acylamidase from Pseudomonas fluorescens (2002), Biochemistry, 41, 991-1000.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
p-nitroacetanilide | 25°C, pH 8 | Pseudomonas fluorescens |  |
| 0.018 | - |
4-nitrophenyl acetate | 25°C, pH 8 | Pseudomonas fluorescens | |
| 0.02 | - |
p-nitroacetanilide | 25°C, pH 10 | Pseudomonas fluorescens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Pseudomonas fluorescens | ? | - |
? | |
| p-nitroacetanilide + H2O | studies on the mechanism of the reaction | Pseudomonas fluorescens | p-nitroaniline + acetate | - |
r | |
| p-nitrophenyl acetate + H2O | - |
Pseudomonas fluorescens | p-nitrophenol + acetate | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AAA | - |
Pseudomonas fluorescens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 11 | maximal activity in this range of pH, enzyme is inactive below pH 7.5 | Pseudomonas fluorescens |
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