BRENDA - Enzyme Database show
show all sequences of 3.5.1.13

The active site of an inducible arylacylamidase from Pseudomonas acidovorans

Heymann, E.; Rix, H.; Int. J. Pept. Protein Res. 11, 59-64 (1978)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
diisopropylphosphofluoridate
-
Delftia acidovorans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Delftia acidovorans
inducible enzyme
?
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Delftia acidovorans
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
amino acid sequence around the reactive serine residue is Gly-Ser-Ile
208981
Delftia acidovorans
?
-
-
-
-
additional information
inducible enzyme
208981
Delftia acidovorans
?
-
-
-
?
p-nitroacetanilide + H2O
i.e. 4-nitroacetanilide
208981
Delftia acidovorans
4-nitroaniline + acetate
-
-
-
?
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
diisopropylphosphofluoridate
-
Delftia acidovorans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Delftia acidovorans
inducible enzyme
?
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
amino acid sequence around the reactive serine residue is Gly-Ser-Ile
208981
Delftia acidovorans
?
-
-
-
-
additional information
inducible enzyme
208981
Delftia acidovorans
?
-
-
-
?
p-nitroacetanilide + H2O
i.e. 4-nitroacetanilide
208981
Delftia acidovorans
4-nitroaniline + acetate
-
-
-
?
Other publictions for EC 3.5.1.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
733190
Ma
Nitrilase superfamily aryl acy ...
Streptomyces sp. 211726, Streptomyces sp.
Appl. Microbiol. Biotechnol.
98
8583-8590
2014
2
-
1
-
3
-
5
-
-
1
1
2
-
5
-
-
1
-
-
1
3
-
10
1
1
1
3
-
1
1
1
-
-
-
-
2
-
1
-
-
3
-
-
5
-
-
-
1
1
2
-
-
-
1
-
1
3
-
10
1
1
1
3
-
1
1
1
-
-
1
1
-
-
-
718672
Shen
Cloning and characterization o ...
Paracoccus sp. M1-1
Appl. Microbiol. Biotechnol.
94
1007-1018
2012
-
-
1
-
3
-
17
3
-
3
2
-
-
3
-
-
1
-
-
-
1
1
7
1
1
1
4
3
1
3
4
-
-
1
-
-
-
1
-
-
3
-
-
17
-
3
-
3
2
-
-
-
-
1
-
-
1
1
7
1
1
1
4
3
1
3
4
1
-
2
2
-
3
3
720273
Acero
-
Two-step enzymatic functionali ...
Nocardia farcinica, Nocardia farcinica IFM 10152
J. Mol. Catal. B
79
54-60
2012
-
-
-
-
-
-
-
1
-
-
1
-
-
3
-
-
1
-
-
-
1
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
-
1
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
713035
Ko
Molecular characterization of ...
bacterium CSBL00001
Mol. Cells
29
485-492
2010
-
-
1
-
-
-
5
4
-
-
-
5
-
2
-
-
-
-
-
-
-
-
7
-
1
-
4
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
5
-
4
-
-
-
5
-
-
-
-
-
-
-
-
7
-
1
-
4
-
2
-
-
-
-
-
-
-
-
-
696876
Heumann
A novel aryl acylamidase from ...
Nocardia farcinica
Biotechnol. Bioeng.
102
1003-1011
2009
-
-
-
-
-
-
4
2
-
-
3
-
-
4
-
-
1
-
-
-
1
2
13
1
1
-
1
2
1
1
-
-
4
-
-
-
-
-
-
-
-
-
-
4
4
2
-
-
3
-
-
-
-
1
-
-
1
2
13
1
1
-
1
2
1
1
-
-
-
-
-
-
-
-
697902
Montenegro
Hydrolysis of acetylthiocoline ...
Bos taurus
FEBS J.
276
2074-2083
2009
-
-
-
-
-
-
2
2
-
-
-
-
-
3
-
-
1
-
-
1
1
-
2
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
1
-
1
1
-
2
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
711318
Rajesh
The aryl acylamidase activity ...
Gallus gallus
Biochimie
91
1087-1094
2009
-
-
-
-
-
-
4
-
-
-
-
-
-
3
-
-
1
-
-
3
-
-
1
-
1
-
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
-
3
4
-
-
-
-
-
-
-
-
-
1
-
3
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
711319
Rajesh
High aryl acylamidase activity ...
Bungarus, Daboia russelii, Echis carinatus, Naja oxiana
Biochimie
91
1450-1456
2009
-
4
-
-
-
-
2
-
4
-
-
-
-
7
-
-
-
-
-
4
-
-
4
-
4
-
-
-
4
-
-
-
-
-
-
-
4
-
-
-
-
-
-
2
-
-
4
-
-
-
-
-
-
-
-
4
-
-
4
-
4
-
-
-
4
-
-
-
-
4
4
-
-
-
678541
Montenegro
Human butyrylcholinesterase co ...
Homo sapiens
Biol. Chem.
389
425-432
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
4
7
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
4
7
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
679183
Montenegro
The level of aryl acylamidase ...
Homo sapiens
Chem. Biol. Interact.
175
336-339
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
3
3
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
3
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697309
Li
Binding and hydrolysis of soma ...
Homo sapiens
Chem. Res. Toxicol.
21
421-431
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697867
Masson
Kinetic analysis of effector m ...
Homo sapiens
FEBS J.
275
2617-2631
2008
2
-
1
-
1
-
3
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
10
-
-
2
-
1
-
-
1
-
-
3
10
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
678058
Peterson
The dependence of enzyme activ ...
Pseudomonas fluorescens
Biochem. J.
403
615
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
679287
Boopathy
Human serum cholinesterase fro ...
Homo sapiens
Clin. Chim. Acta
380
151-156
2007
-
1
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
5
21
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
5
21
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
681110
Masson
Aryl acylamidase activity of h ...
Homo sapiens
J. Enzyme Inhib. Med. Chem.
22
463-469
2007
-
-
-
-
-
-
1
1
-
-
-
-
-
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667330
Manoharan
Diisopropylfluorophosphate-sen ...
Homo sapiens
Arch. Biochem. Biophys.
452
186-188
2006
-
-
-
-
-
-
1
-
1
-
-
1
-
2
-
-
-
-
-
6
1
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
6
1
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
667928
Darvesh
On the active site for hydroly ...
Homo sapiens, Pseudomonas fluorescens
Bioorg. Med. Chem.
14
4586-4599
2006
-
-
-
-
-
-
-
8
-
-
-
1
-
3
-
-
-
1
-
1
2
-
27
1
2
-
-
11
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
1
-
-
-
-
-
1
2
-
27
1
2
-
-
11
2
-
-
-
-
-
-
-
-
-
668239
Allebrandt
Expression of acetylcholineste ...
Danio rerio
Chem. Biol. Interact.
157-158
353-355
2005
-
-
-
-
-
-
2
-
-
-
-
1
-
3
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
670800
Boopathy
Aryl acylamidase activity on a ...
Gallus gallus
Protein J.
23
325-333
2004
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
1
1
-
3
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
-
3
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
657938
Stein
Enzymatic hydrolysis of p-nitr ...
Pseudomonas fluorescens
Biochemistry
41
991-1000
2002
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
208992
Weitnauer
Aryl acylamidase activity exhi ...
Equus caballus, Gallus gallus
Neurosci. Lett.
254
153-156
1998
-
-
-
-
-
-
2
-
-
-
-
-
-
6
-
-
-
-
-
4
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
4
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
208994
Costagli
Inhibition of cholinesterase-a ...
Electrophorus electricus, Equus caballus
Biochem. Pharmacol.
55
1733-1737
1998
-
-
-
-
-
-
28
-
-
-
-
-
-
4
-
-
-
-
-
3
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
28
-
-
-
-
-
-
-
-
-
-
-
3
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
208993
Fukuda
Crystallization and preliminar ...
Tulipa gesneriana
Acta Crystallogr. Sect. D
53
342-344
1997
-
-
-
1
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
208991
Hoagland
-
Rhizobacteria with exceptional ...
Pseudomonas sp., Pseudomonas sp. RA2
Pestic. Biochem. Physiol.
52
190-200
1995
-
-
-
-
-
-
1
6
-
-
-
2
-
2
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
6
-
-
-
2
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
208971
Villarreal
A structure-activity study wit ...
Acinetobacter sp., Acinetobacter sp. DV1, Arthrobacter sp., Corynebacterium sp., Corynebacterium sp. DAK12
Appl. Environ. Microbiol.
60
3939-3944
1994
-
-
-
-
-
-
-
-
-
-
-
10
-
5
-
-
-
-
-
-
-
-
10
-
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208996
Checler
Cholinesterases display genuin ...
Electrophorus electricus
J. Neurochem.
62
756-763
1994
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208997
Jayanthi
Cholinesterases exhibiting ary ...
Homo sapiens
Clin. Chim. Acta
205
157-166
1992
1
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208989
Khanna
Characteristics of a cytosolic ...
Felis catus, Homo sapiens, Mus musculus, Rattus norvegicus
J. Pharmacol. Exp. Ther.
262
1225-1231
1991
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7
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1
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208990
Jun
-
The propanil hydrolyzing enzym ...
Oryza alta, Oryza australiensis, Oryza barthii, Oryza coarctata, Oryza collina, Oryza eichingeri, Oryza glaberrima, Oryza grandiglumis, Oryza latifolia, Oryza longiglumis, Oryza longistaminata, Oryza malabarensis, Oryza meyeriana var. granulata, Oryza minuta, Oryza officinalis, Oryza punctata, Oryza ridleyi, Oryza rufipogon, Oryza sativa
Pestic. Biochem. Physiol.
38
26-33
1990
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12
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19
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12
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12
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19
-
12
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12
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208995
Vaughan
Aryl acylamidase from Rhodococ ...
Rhodococcus erythropolis
Appl. Microbiol. Biotechnol.
34
42-46
1990
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1
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3
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1
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208969
Boopathy
Chemical modification of the b ...
Homo sapiens
Eur. J. Biochem.
151
351-360
1985
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7
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208970
Majumdar
Chemical modification of acety ...
Electrophorus electricus, Ovis aries
Biochemistry
23
4088-4093
1984
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5
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3
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5
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2
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2
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208972
Gaynor
Subcellular localization of ri ...
Oryza sativa
Plant Physiol.
72
80-85
1983
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2
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1
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3
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3
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208973
Hammond
Purification and properties of ...
Pseudomonas fluorescens
Eur. J. Biochem.
132
651-655
1983
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3
2
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2
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2
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1
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2
2
1
1
1
1
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1
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1
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1
2
2
1
1
1
1
-
1
1
-
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-
208974
Hsu
Brain aryl acylamidase ...
Rattus norvegicus
Int. J. Biochem.
14
1037-1042
1982
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1
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4
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208975
Hsu
Rat brain aryl acylamidase: fu ...
Rattus norvegicus
Int. J. Biochem.
14
581-584
1982
7
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9
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9
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208976
Majumdar
Essential and non-essential ph ...
Ovis aries
FEBS Lett.
146
335-338
1982
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1
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1
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208977
George
The aryl acylamidases and thei ...
Homo sapiens
Eur. J. Biochem.
121
177-186
1981
1
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2
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2
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5
2
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1
1
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-
-
208978
George
The identity of the serotonin- ...
Electrophorus electricus, Homo sapiens, Ovis aries
Eur. J. Biochem.
111
511-524
1980
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15
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3
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6
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3
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8
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3
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15
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3
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8
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3
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-
208979
Oommen
The association of the seroton ...
Platyrrhini
Eur. J. Biochem.
94
135-143
1979
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15
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2
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1
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15
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1
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2
1
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2
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-
-
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-
208980
Nakadai
-
Properties of arylamidase from ...
Aspergillus oryzae
Agric. Biol. Chem.
42
1291-1292
1978
-
-
-
-
-
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11
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1
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1
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13
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2
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4
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1
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11
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1
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13
-
2
-
3
-
4
-
1
-
-
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-
-
-
-
208981
Heymann
The active site of an inducibl ...
Delftia acidovorans
Int. J. Pept. Protein Res.
11
59-64
1978
-
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1
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1
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1
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3
-
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-
-
-
-
-
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-
208982
Hsu
Rat brain aryl acylamidase: mu ...
Rattus norvegicus
Life Sci.
20
857-866
1977
-
-
-
-
-
-
4
-
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2
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1
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1
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2
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4
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1
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1
-
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-
2
-
-
-
-
-
-
-
-
-
208983
Fujimoto
Serotonin-sensitive aryl acyla ...
Electrophorus electricus, Sus scrofa
FEBS Lett.
71
121-123
1976
-
-
-
-
-
-
2
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2
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1
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3
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2
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1
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3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
208984
Paul
Rat brain de-acetylating activ ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
70
207-211
1976
-
-
-
-
-
-
7
-
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1
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1
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7
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
208985
Hoagland
-
Hydrolysis of 3',4'-dichloropr ...
Taraxacum officinale
Phytochemistry
14
383-386
1975
-
-
-
-
-
-
9
-
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1
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1
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1
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10
-
1
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3
-
1
1
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1
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9
1
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1
-
1
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10
-
1
-
3
-
1
1
-
-
-
-
-
-
-
-
208986
Hoagland
The purification and propertie ...
Glycine max
Can. J. Biochem.
52
903-910
1974
-
-
-
-
-
-
8
1
-
-
1
-
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1
-
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1
-
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1
1
1
13
-
1
-
1
-
1
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-
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-
-
-
8
-
1
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1
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1
-
1
1
1
13
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
208987
Fujimoto
Serotonin-sensitive aryl acyla ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
61
72-74
1974
-
-
-
-
-
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2
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2
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2
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2
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-
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-
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-
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-
-
208988
Engelhardt
Purification and properties of ...
Lysinibacillus sphaericus
Appl. Microbiol.
26
709-718
1973
-
-
-
-
-
1
10
4
-
-
1
1
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2
-
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1
-
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1
5
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3
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1
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10
-
4
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1
1
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1
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1
5
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3
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