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Literature summary for 3.5.1.13 extracted from
- Purification and properties of aryl acylamidase from Pseudomonas fluorescens ATCC 39004 (1983), Eur. J. Biochem., 132, 651-655.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 1.0 mM AgNO3, complete inactivation | Pseudomonas fluorescens |  |
| CdCl2 | 1.0 mM, complete inactivation | Pseudomonas fluorescens | |
| HgCl2 | 1.0 mM, complete inactivation | Pseudomonas fluorescens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0061 | - |
hydroxyacetanilide | - |
Pseudomonas fluorescens | |
| 0.069 | - |
p-nitroacetanilide | - |
Pseudomonas fluorescens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
gel filtration | Pseudomonas fluorescens |
| 52500 | - |
1 * 52500, SDS-PAGE | Pseudomonas fluorescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
ATCC 39004 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas fluorescens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 319.2 | - |
- |
Pseudomonas fluorescens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C or 20°C, stable for 420 min | Pseudomonas fluorescens |
| 4°C, storage after freeze-drying in buffer containing 5% lactose, about 12% loss of activity | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hydroxyacetanilide + H2O | - |
Pseudomonas fluorescens | hydroxyaniline + acetate | - |
? | |
| p-nitroacetanilide + H2O | - |
Pseudomonas fluorescens | 4-nitroaniline + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 52500, SDS-PAGE | Pseudomonas fluorescens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Pseudomonas fluorescens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 55 | 25°C: about 55% of maximal activity, 55°C: about 40% of maximal activity | Pseudomonas fluorescens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
20 min, complete inactivation | Pseudomonas fluorescens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
- |
Pseudomonas fluorescens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 12 | pH 7.0: about 50% of maximal activity, pH 12.0: about 30% of maximal activity | Pseudomonas fluorescens |
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