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Literature summary for 3.5.1.129 extracted from

  • Taylor, Z.; Brown, H.; Holden, H.; Raushel, F.
    Biosynthesis of nucleoside diphosphoramidates in Campylobacter jejuni (2017), Biochemistry, 56, 6079-6082 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.028
-
N5-(cytidine 5'-diphosphoramidyl)-L-glutamine pH 8.0, 25°C Campylobacter jejuni subsp. jejuni serotype O:2

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni subsp. jejuni serotype O:2 Q0P8J7
-
-
Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 Q0P8J7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information L-glutamine phosphate is a very poor substrate Campylobacter jejuni subsp. jejuni serotype O:2 ?
-
?
additional information L-glutamine phosphate is a very poor substrate Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 ?
-
?
N5-(cytidine 5'-diphosphoramidyl)-L-glutamine + H2O
-
Campylobacter jejuni subsp. jejuni serotype O:2 cytidine 5'-diphosphoramidate + L-glutamate
-
?
N5-(cytidine 5'-diphosphoramidyl)-L-glutamine + H2O
-
Campylobacter jejuni subsp. jejuni serotype O:2 ATCC 700819 cytidine 5'-diphosphoramidate + L-glutamate
-
?

Synonyms

Synonyms Comment Organism
Cj1417c
-
Campylobacter jejuni subsp. jejuni serotype O:2
gamma-glutamyl-CDP-amidate hydrolase
-
Campylobacter jejuni subsp. jejuni serotype O:2

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.55
-
N5-(cytidine 5'-diphosphoramidyl)-L-glutamine pH 8.0, 25°C Campylobacter jejuni subsp. jejuni serotype O:2

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Campylobacter jejuni subsp. jejuni serotype O:2

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
20
-
N5-(cytidine 5'-diphosphoramidyl)-L-glutamine pH 8.0, 25°C Campylobacter jejuni subsp. jejuni serotype O:2