Literature summary for 3.5.1.128 extracted from

Peracchi, A.; Veiga-da-Cunha, M.; Kuhara, T.; Ellens, K.W.; Paczia, N.; Stroobant, V.; Seliga, A.K.; Marlaire, S.; Jaisson, S.; Bommer, G.T.; Sun, J.; Huebner, K.; Linster, C.L.; Cooper, A.J.L.; Van Schaftingen, E.
Nit1 is a metabolite repair enzyme that hydrolyzes deaminated glutathione (2017), Proc. Natl. Acad. Sci. USA, 114, E3233-E3242 .

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.045	-	N-(4-oxoglutaryl)-L-cysteinylglycine	pH 8.5, 30°C	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
N-(4-oxoglutaryl)-L-cysteinylglycine + H2O	Homo sapiens	-	2-oxoglutarate + L-cysteinylglycine	-	?
N-(4-oxoglutaryl)-L-cysteinylglycine + H2O	Saccharomyces cerevisiae	-	2-oxoglutarate + L-cysteinylglycine	-	r
N-(4-oxoglutaryl)-L-cysteinylglycine + H2O	Mus musculus	-	2-oxoglutarate + L-cysteinylglycine	-	?
N-(4-oxoglutaryl)-L-cysteinylglycine + H2O	Pseudomonas aeruginosa	-	2-oxoglutarate + L-cysteinylglycine	-	r
N-(4-oxoglutaryl)-L-cysteinylglycine + H2O	Synechocystis sp. PCC 6803	-	2-oxoglutarate + L-cysteinylglycine	-	r
N-(4-oxoglutaryl)-L-cysteinylglycine + H2O	Yersinia enterocolitica	-	2-oxoglutarate + L-cysteinylglycine	-	r
N-(4-oxoglutaryl)-L-cysteinylglycine + H2O	Pseudomonas aeruginosa DSM 22644	-	2-oxoglutarate + L-cysteinylglycine	-	r

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q86X76	-	-
Mus musculus	Q8VDK1	-	-
Pseudomonas aeruginosa	Q9HVU6	-	-
Pseudomonas aeruginosa DSM 22644	Q9HVU6	-	-
Saccharomyces cerevisiae	P47016	-	-
Synechocystis sp. PCC 6803	P0DP66	-	-
Yersinia enterocolitica	P0DP68	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
HAP-1 cell	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
6.5	-	pH 8.5, 37°C	Yersinia enterocolitica
7.8	-	pH 8.5, 37°C	Synechocystis sp. PCC 6803
8.71	-	pH 8.5, 37°C	Saccharomyces cerevisiae
13.6	-	pH 8.5, 37°C	Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
N-(4-oxoglutaryl)-L-cysteinylglycine + H2O	-	Homo sapiens	2-oxoglutarate + L-cysteinylglycine	-	?
N-(4-oxoglutaryl)-L-cysteinylglycine + H2O	-	Saccharomyces cerevisiae	2-oxoglutarate + L-cysteinylglycine	-	?
N-(4-oxoglutaryl)-L-cysteinylglycine + H2O	-	Saccharomyces cerevisiae	2-oxoglutarate + L-cysteinylglycine	-	r
N-(4-oxoglutaryl)-L-cysteinylglycine + H2O	-	Mus musculus	2-oxoglutarate + L-cysteinylglycine	-	?
N-(4-oxoglutaryl)-L-cysteinylglycine + H2O	-	Pseudomonas aeruginosa	2-oxoglutarate + L-cysteinylglycine	-	?
N-(4-oxoglutaryl)-L-cysteinylglycine + H2O	-	Pseudomonas aeruginosa	2-oxoglutarate + L-cysteinylglycine	-	r
N-(4-oxoglutaryl)-L-cysteinylglycine + H2O	-	Synechocystis sp. PCC 6803	2-oxoglutarate + L-cysteinylglycine	-	?
N-(4-oxoglutaryl)-L-cysteinylglycine + H2O	-	Synechocystis sp. PCC 6803	2-oxoglutarate + L-cysteinylglycine	-	r
N-(4-oxoglutaryl)-L-cysteinylglycine + H2O	-	Yersinia enterocolitica	2-oxoglutarate + L-cysteinylglycine	-	?
N-(4-oxoglutaryl)-L-cysteinylglycine + H2O	-	Yersinia enterocolitica	2-oxoglutarate + L-cysteinylglycine	-	r
N-(4-oxoglutaryl)-L-cysteinylglycine + H2O	-	Pseudomonas aeruginosa DSM 22644	2-oxoglutarate + L-cysteinylglycine	-	?
N-(4-oxoglutaryl)-L-cysteinylglycine + H2O	-	Pseudomonas aeruginosa DSM 22644	2-oxoglutarate + L-cysteinylglycine	-	r

Synonyms

Synonyms	Comment	Organism
deaminated glutathione amidase	-	Homo sapiens
deaminated glutathione amidase	-	Saccharomyces cerevisiae
NIT1	-	Homo sapiens
NIT1	-	Saccharomyces cerevisiae
NIT1	-	Mus musculus
NIT1	-	Synechocystis sp. PCC 6803
NIT1	-	Yersinia enterocolitica
NIT2	-	Saccharomyces cerevisiae
nitrilase-like protein 1	-	Homo sapiens
nitrilase-like protein 1	-	Saccharomyces cerevisiae
nitrilase-like protein 1	-	Mus musculus
PA4475	-	Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.6	-	N-(4-oxoglutaryl)-L-cysteinylglycine	pH 8.5, 30°C	Saccharomyces cerevisiae

General Information

General Information	Comment	Organism
physiological function	deaminated glutathione, i.e. N-(4-oxoglutaryl)-L-cysteinylglycine, is exreted in large amounts in the urine of Nit1 knock-out mice	Mus musculus
physiological function	Nit1-KO mutant cells accumulate deaminated GSH	Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
80	-	N-(4-oxoglutaryl)-L-cysteinylglycine	pH 8.5, 30°C	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)