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Literature summary for 3.5.1.126 extracted from

  • Hicks, K.A.; Ealick, S.E.
    Biochemical and structural characterization of Klebsiella pneumoniae oxamate amidohydrolase in the uric acid degradation pathway (2016), Acta Crystallogr. Sect. D, 72, 808-816.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.126

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Klebsiella pneumoniae subsp. pneumoniae

Crystallization (Commentary)

Crystallization (Comment) Organism
to 2.9 A resolution. This active site is located in a solvent-exposed cleft with nonpolar amino acids lining the binding cleft, including Met423, Gly424 and Gly425. Other amino acids in the active site are Thr342, Ser360, Tyr362 and Leu405 Klebsiella pneumoniae subsp. pneumoniae

Protein Variants

Protein Variants Comment Organism
S360A mutant is able to autoprocess, but is catalytically inactive Klebsiella pneumoniae subsp. pneumoniae
T342A mutant does not autoprocess into a mature heterodimer and is inactive Klebsiella pneumoniae subsp. pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
-
 Klebsiella pneumoniae subsp. pneumoniae
55000
-
-
 Klebsiella pneumoniae subsp. pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oxamate + H2O Klebsiella pneumoniae subsp. pneumoniae
-
 oxalate + ammonia
-
 ?
oxamate + H2O Klebsiella pneumoniae subsp. pneumoniae ATCC 700721
-
 oxalate + ammonia
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae subsp. pneumoniae A6T9C8
-
-
Klebsiella pneumoniae subsp. pneumoniae ATCC 700721 A6T9C8
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification protein autoprocessed from an inactive precursor to form a heterodimer, resulting in a 35.5 kDa alpha subunit and a 20 kDa beta subunit Klebsiella pneumoniae subsp. pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxamate + H2O
-
 Klebsiella pneumoniae subsp. pneumoniae oxalate + ammonia
-
 ?
oxamate + H2O
-
 Klebsiella pneumoniae subsp. pneumoniae ATCC 700721 oxalate + ammonia
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, precursor protein, x *35500, alpha subunit, x * 20000, beta subunit, SDS-PAGE Klebsiella pneumoniae subsp. pneumoniae

Synonyms

Synonyms Comment Organism
HpxW
-
 Klebsiella pneumoniae subsp. pneumoniae
KPN_01768
-
 Klebsiella pneumoniae subsp. pneumoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.5
-
 oxamate pH 8.5, 22°C Klebsiella pneumoniae subsp. pneumoniae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.159
-
 oxamate pH 8.5, 22°C Klebsiella pneumoniae subsp. pneumoniae