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Literature summary for 3.5.1.121 extracted from

  • Kwon, Y.; Balogh, S.; Davydov, I.; Kashina, A.; Yoon, J.; Xie, Y.; Gaur, A.; Hyde, L.; Denenberg, V.; Varshavsky, A.
    Altered activity, social behavior, and spatial memory in mice lacking the NTAN1p amidase and the asparagine branch of the N-end rule pathway (2000), Mol. Cell. Biol., 20, 4135-4148.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.121

Cloned(Commentary)

Cloned (Comment) Organism
gene Ntan1, chromosomal localization and expression analysis, recombinant expression of N-terminally GFP-tagged 65-kDa in NTAN1pmouse 3T3 cells Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information construction and analysis of mouse strains lacking the asparagine-specific N-terminal amidase (NtN-amidase), encoded by the Ntan1 gene, by deletion-disruption of the mouse Ntan1 gene Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-terminal L-asparaginyl-[protein] + H2O Mus musculus
-
 N-terminal L-aspartyl-[protein] + NH3
-
 ?
N-terminal L-asparaginyl-[protein] + H2O Mus musculus C129
-
 N-terminal L-aspartyl-[protein] + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus Q64311
-
-
Mus musculus C129 Q64311
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Mus musculus
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embryo in wild-type embryos, Ntan1 is strongly expressed in the branchial arches and in the tail and limb buds Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-terminal L-asparaginyl-[protein] + H2O
-
 Mus musculus N-terminal L-aspartyl-[protein] + NH3
-
 ?
N-terminal L-asparaginyl-[protein] + H2O Asp-DHFR protein bearing Asn at its N-terminus is used as substrate Mus musculus N-terminal L-aspartyl-[protein] + NH3
-
 ?
N-terminal L-asparaginyl-[protein] + H2O
-
 Mus musculus C129 N-terminal L-aspartyl-[protein] + NH3
-
 ?
N-terminal L-asparaginyl-[protein] + H2O Asp-DHFR protein bearing Asn at its N-terminus is used as substrate Mus musculus C129 N-terminal L-aspartyl-[protein] + NH3
-
 ?

Synonyms

Synonyms Comment Organism
NTAN1
-
 Mus musculus
NTAN1p amidase
-
 Mus musculus

General Information

General Information Comment Organism
malfunction Ntan1-/- mice lack NtN-amidase and the asparagine-specific branch of the N-end rule pathway. The phenoytpe shows altered activity, social behavior, and spatial memory in mice lacking the NTAN1p amidase and the asparagine branch of the N-end rule pathway. The Ntan1-/- mouse strains lacking the NtN-amidase activity but retaining glutamine-specific NtQ amidase, EC 3.5.1.122, show that the two enzymes are encoded by different genes. Among the normally short-lived N-end rule substrates, only those bearing N-terminal asparagine became long-lived in Ntan1-/- fibroblasts. The Ntan1-/- mice are fertile and outwardly normal but differ from their congenic wild-type counterparts in spontaneous activity, spatial memory, and a socially conditioned exploratory phenotype Mus musculus
metabolism the enzyme is involved in the mammalian N-end rule pathway. The N-end rule relates the in vivo half-life of a protein to the identity of its N-terminal residue. N-terminal asparagine and glutamine are tertiary destabilizing residues, in that they are enzymatically deamidated to yield secondary destabilizing residues aspartate and glutamate, which are conjugated to arginine, a primary destabilizing residue Mus musculus