Cloned (Comment) | Organism |
---|---|
gene NTAN1, phylogenetic analysis, recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes with a C-terminal strepII-tag or a FLAG-strepII tandem affinity tag in Escherichia coli strain BL21(DE3), method improvement | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C160A | site-directed mutagenesis, the mutant show unaltered activity compared to the wild-type enzyme | Homo sapiens |
C75A | site-directed mutagenesis, inactive mutant | Homo sapiens |
C75S | site-directed mutagenesis, inactive mutant | Homo sapiens |
C75T | site-directed mutagenesis, inactive mutant | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | partial inhibition at 0.050 mM | Homo sapiens | |
Cu2+ | complete inhibition at 0.050 mM | Homo sapiens | |
Mn2+ | partial inhibition at 0.050 mM | Homo sapiens | |
additional information | no inhibition by Mg2+, Ca2+, EDTA, or D-desthiobiotin | Homo sapiens | |
Ni2+ | partial inhibition at 0.050 mM | Homo sapiens | |
Zn2+ | complete inhibition at 0.050 mM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetic modelling | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-terminal L-asparaginyl-[protein] + H2O | Homo sapiens | - |
N-terminal L-aspartyl-[protein] + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q96AB6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by affinity cromatography and ultrafiltration, method improvement | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | hNTAN1 is highly selective for the hydrolysis of N-terminal peptidyl L-Asn but fails to deamidate free L-Asn or L-Gln, N-terminal peptidyl L-Gln, or acetylated N-terminal peptidyl L-Asn | Homo sapiens | ? | - |
? | |
N-terminal L-asparaginyl-[protein] + H2O | - |
Homo sapiens | N-terminal L-aspartyl-[protein] + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
N-terminal asparagine amidohydrolase | - |
Homo sapiens |
NTAN1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | hNTAN1 is shown to possess a critical Cys residue that is absolutely required for catalysis, corroborated in part by abolishment of activity through the Cys75Ala point mutation | Homo sapiens |
metabolism | the enzyme is involved in the mammalian N-end rule pathway | Homo sapiens |
physiological function | the enzymatic deamidation of N-terminal L-Asn by N-terminal asparagine amidohydrolase (NTAN1) is a feature of the ubiquitin-dependent N-end rule pathway of protein degradation, which relates the in vivo half-life of a protein to the identity of its N-terminal residue. hNTAN1 is shown to possess a critical Cys residue that is absolutely required for catalysis, corroborated in part by abolishment of activity through the Cys75Ala point mutation. The exposure of a conserved L-Pro at the N-terminus of hNTAN1 following removal of the initiating L-Met is important for the function of the enzyme | Homo sapiens |