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Literature summary for 3.5.1.12 extracted from
- Enkephalin hydrolysis by human serum biotinidase (1991), Biochim. Biophys. Acta, 1074, 433-438.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Homo sapiens |  |
| amastatin | peptidase inhibitor | Homo sapiens | |
| bestatin | peptidase inhibitor | Homo sapiens | |
| Hg2+ | - |
Homo sapiens | |
| puromycin | peptidase inhibitor | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0051 | - |
epsilon-N-biotinyl-L-lysine | - |
Homo sapiens | |
| 0.03 | - |
p-(N-biotinylamino)benzoic acid | - |
Homo sapiens | |
| 0.099 | - |
Met-enkephalin amide | - |
Homo sapiens | |
| 0.119 | - |
Leu-enkephalin amide | - |
Homo sapiens | |
| 0.132 | - |
dynorphin A (1-7) | - |
Homo sapiens | |
| 0.167 | - |
dynorphin A (1-6) | - |
Homo sapiens | |
| 0.303 | - |
Met-enkephalin | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Homo sapiens | 5737 | - |
| cytoplasm | - |
Sus scrofa | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 76000 | - |
- |
Homo sapiens |
| 76000 | - |
glycoprotein with 59000 Da polypeptide core , SDS-PAGE | Sus scrofa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| biotin amide + H2O | Homo sapiens | - |
? | - |
? | |
| biotin amide + H2O | Sus scrofa | - |
? | - |
? | |
| epsilon-N-biotinyl-L-lysine + H2O | Homo sapiens | - |
? | - |
? | |
| epsilon-N-biotinyl-L-lysine + H2O | Sus scrofa | - |
? | - |
? | |
| Leu-enkephalin + H2O | Homo sapiens | - |
? | - |
? | |
| Met-enkephalin + H2O | Homo sapiens | suitable natural substrate for biotinidase | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
human | - |
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blood | serum, plasma, leukocytes | Homo sapiens | - |
| blood | serum, plasma, leukocytes | Sus scrofa | - |
| brain | - |
Homo sapiens | - |
| brain | - |
Sus scrofa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| biotin amide + H2O | - |
Homo sapiens | ? | - |
? | |
| biotin amide + H2O | - |
Sus scrofa | ? | - |
? | |
| dynorphin A (1-6) + H2O | - |
Homo sapiens | ? | - |
? | |
| dynorphin A (1-7) + H2O | - |
Homo sapiens | ? | - |
? | |
| epsilon-N-biotinyl-L-lysine + H2O | - |
Homo sapiens | biotin + L-lysine | - |
ir | |
| epsilon-N-biotinyl-L-lysine + H2O | - |
Sus scrofa | biotin + L-lysine | - |
ir | |
| epsilon-N-biotinyl-L-lysine + H2O | - |
Homo sapiens | ? | - |
? | |
| epsilon-N-biotinyl-L-lysine + H2O | - |
Sus scrofa | ? | - |
? | |
| Leu-enkephalin + H2O | - |
Homo sapiens | ? | - |
? | |
| Leu-enkephalin amide + H2O | - |
Homo sapiens | ? | - |
? | |
| Met-enkephalin + H2O | - |
Homo sapiens | ? | - |
? | |
| Met-enkephalin + H2O | suitable natural substrate for biotinidase | Homo sapiens | ? | - |
? | |
| Met-enkephalin amide + H2O | - |
Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| biocytin hydrolyzing amidase | - |
Homo sapiens |
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