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Literature summary for 3.5.1.119 extracted from

  • Striebel, F.; Imkamp, F.; Sutter, M.; Steiner, M.; Mamedov, A.; Weber-Ban, E.
    Bacterial ubiquitin-like modifier Pup is deamidated and conjugated to substrates by distinct but homologous enzymes (2009), Nat. Struct. Mol. Biol., 16, 647-651.
    View publication on PubMed
Search for more literature for 3.5.1.119

Cloned(Commentary)

Cloned (Comment) Organism
the dop gene is cloned with a C-terminal His6-tag, expression in Escherichia coli Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[prokaryotic ubiquitin-like protein]-L-glutamine + H2O Mycobacterium tuberculosis the enzyme deamidates prokaryotic ubiquitin-like protein (Pup) on the C-terminal glutamine, thereby rendering Pup competent for conjugation [prokaryotic ubiquitin-like protein]-L-glutamate + NH3
-
 ?
[prokaryotic ubiquitin-like protein]-L-glutamine + H2O Mycobacterium tuberculosis ATCC 25618 the enzyme deamidates prokaryotic ubiquitin-like protein (Pup) on the C-terminal glutamine, thereby rendering Pup competent for conjugation [prokaryotic ubiquitin-like protein]-L-glutamate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WNU9
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-
Mycobacterium tuberculosis ATCC 25618 P9WNU9
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-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme expressed in Escherichia coli Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[prokaryotic ubiquitin-like protein]-L-glutamine + H2O the enzyme deamidates prokaryotic ubiquitin-like protein (Pup) on the C-terminal glutamine, thereby rendering Pup competent for conjugation Mycobacterium tuberculosis [prokaryotic ubiquitin-like protein]-L-glutamate + NH3
-
 ?
[prokaryotic ubiquitin-like protein]-L-glutamine + H2O
-
 Mycobacterium tuberculosis [prokaryotic ubiquitin-like protein]-L-glutamate + NH3
-
 ?
[prokaryotic ubiquitin-like protein]-L-glutamine + H2O the enzyme deamidates prokaryotic ubiquitin-like protein (Pup) on the C-terminal glutamine, thereby rendering Pup competent for conjugation Mycobacterium tuberculosis ATCC 25618 [prokaryotic ubiquitin-like protein]-L-glutamate + NH3
-
 ?
[prokaryotic ubiquitin-like protein]-L-glutamine + H2O
-
 Mycobacterium tuberculosis ATCC 25618 [prokaryotic ubiquitin-like protein]-L-glutamate + NH3
-
 ?

Synonyms

Synonyms Comment Organism
Dop
-
 Mycobacterium tuberculosis
Rv2112 locus name Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
ATP deamidation requires ATP as a cofactor but not its hydrolysis Mycobacterium tuberculosis

General Information

General Information Comment Organism
metabolism the enzyme is involved in pupylation Mycobacterium tuberculosis