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Literature summary for 3.5.1.117 extracted from

  • akehara, I.; Fujii, T.; Tanimoto, Y.; Kato, D.I.; Takeo, M.; Negoro, S.
    Metabolic pathway of 6-aminohexanoate in the nylon oligomer-degrading bacterium Arthrobacter sp. KI72 identification of the enzymes responsible for the conversion of 6-aminohexanoate to adipate (2018), Appl. Microbiol. Biotechnol., 102, 801-814 .
    View publication on PubMed
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Application

Application Comment Organism
synthesis bio-based conversion from wastes/byproducts of nylon-6 to adipate (nylon-66 monomer) is possible, when the NADPH generated by the NylE reaction is re-oxidized to NADP+ by coupling a suitable oxidoreductase reaction and the enzyme activity is high enough at a practical level Paenarthrobacter ureafaciens

Cloned(Commentary)

Cloned (Comment) Organism
gene nylC, phylogenetic analysis and tree Paenarthrobacter ureafaciens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-aminohexanoate oligomer + H2O Paenarthrobacter ureafaciens
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 6-aminohexanoate dimer + ?
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 ?

Organism

Organism UniProt Comment Textmining
Paenarthrobacter ureafaciens P07062 i.e. Flacobacterium sp. K172
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Source Tissue

Source Tissue Comment Organism Textmining
additional information Arthrobacter sp. strain KI72 grows on a 6-aminohexanoate oligomer, which is a by-product of nylon-6 manufacturing, as a sole source of carbon and nitrogen Paenarthrobacter ureafaciens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-aminohexanoate oligomer + H2O
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 Paenarthrobacter ureafaciens 6-aminohexanoate dimer + ?
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 ?

Synonyms

Synonyms Comment Organism
Ahx oligomer hydrolase
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 Paenarthrobacter ureafaciens
endo-type 6-aminohexanoate oligomer hydrolase
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 Paenarthrobacter ureafaciens
NylC
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 Paenarthrobacter ureafaciens
nylon hydrolase
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 Paenarthrobacter ureafaciens

General Information

General Information Comment Organism
metabolism enzymatic degradation of 6-aminohexanoate and its oligomers in Arthrobacter sp. KI7 by the Nyl enzymes. A 6-aminohexanoate oligomer is converted to adipate, pathway overview. 6-Aminohexanoate oligomers are almost completely converted to the monomers (Ahx) by the three nylon oligomer-degrading enzymes, NylA, NylB, and NylC. 6-Aminohexanoate aminotransferase (NylD1) catalyzes the reaction of 6-aminohexanoate to adipate semialdehyde using alpha-ketoglutarate, pyruvate, and glyoxylate as amino acceptors, generating glutamate, alanine, and glycine, respectively. The reaction requires pyridoxal phosphate (PLP) as a cofactor. For further metabolism, adipate semialdehyde dehydrogenase (NylE1) catalyzes the oxidative reaction of adipate semialdehyde to adipate using NADP+ as a cofactor Paenarthrobacter ureafaciens