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Literature summary for 3.5.1.116 extracted from
- Ureidoglycolate amidohydrolase from developing French bean fruits (Phaseolus vulgaris [L.].) (1991), Arch. Biochem. Biophys., 287, 151-159.
General Stability
| General Stability | Organism |
|---|---|
| Mn2+ stabilizes against heat inactivation | Phaseolus vulgaris |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Phaseolus vulgaris |  |
| Ni2+ | slight | Phaseolus vulgaris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
ureidoglycolate | in presence of Mn2+ | Phaseolus vulgaris | |
| 5.4 | - |
ureidoglycolate | in absence of Mn2+ | Phaseolus vulgaris | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| peroxisome | - |
Phaseolus vulgaris | 5777 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slight increase of activity | Phaseolus vulgaris | |
| Fe2+ | slight increase of activity | Phaseolus vulgaris | |
| Mg2+ | slight increase of activity | Phaseolus vulgaris | |
| Mn2+ | activates, maximal activity at 0.2 mM | Phaseolus vulgaris | |
| Zn2+ | activates | Phaseolus vulgaris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 300000 | - |
gel filtration | Phaseolus vulgaris |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ureidoglycolate + H2O | Phaseolus vulgaris | - |
glyoxylate + NH3 + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaseolus vulgaris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Phaseolus vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | - |
Phaseolus vulgaris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Phaseolus vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ureidoglycolate + H2O | - |
Phaseolus vulgaris | glyoxylate + NH3 + CO2 | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
15 min, in absence of Mn2+, 60% loss of activity | Phaseolus vulgaris |
| 50 | - |
15 min, in abesence of Mn2+, 88% loss of activity | Phaseolus vulgaris |
| 60 | - |
15 min, in presence of 2 mM Mn2+, stable | Phaseolus vulgaris |
| 70 | - |
15 min, in presence of 2 mM Mn2+, 97% loss of activity | Phaseolus vulgaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | - |
Phaseolus vulgaris |
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Release 2023.1 (January 2023)
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