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Literature summary for 3.5.1.115 extracted from

  • Newton, G.L.; Av-Gay, Y.; Fahey, R.C.
    A novel mycothiol-dependent detoxification pathway in Mycobacteria involving mycothiol S-conjugate amidase (2000), Biochemistry, 39, 10739-10746.
    View publication on PubMed
Search for more literature for 3.5.1.115

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycolicibacterium smegmatis

Inhibitors

Inhibitors Comment Organism Structure
mycothiol amidase activity is decreased by 30, 48 and 89% at 1.0, 3.0, and 10 mM mycothiol, respectively Mycolicibacterium smegmatis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.095
-
 mycothiol bimane derivative at pH 7.5 and 30°C Mycolicibacterium smegmatis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 x * 36000, SDS-PAGE Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a mycothiol S-conjugate + H2O Mycolicibacterium smegmatis the enzyme is highly specific for S-conjugates of mycothiol an N-acetyl L-cysteine-S-conjugate + 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol
-
 ?

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Toyopearl DEAE 650C column chromatography, phenyl Sepharose and Sephadex G-100 gel filtration Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0012
-
 crude extract, at pH 7.5 and 30°C Mycolicibacterium smegmatis
3.3
-
 after 2800fold purification, at pH 7.5 and 30°C Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a mycothiol S-conjugate + H2O the enzyme is highly specific for S-conjugates of mycothiol Mycolicibacterium smegmatis an N-acetyl L-cysteine-S-conjugate + 1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol
-
 ?
additional information no activity with mycothiol, mycothiol disulfide, 2-(N-acetyl-L-cysteinyl)amido-2-deoxy-(alpha,beta)-D-glucopyranoside monobromobiane derivate, and 1-D-myo-inosityl-2-(L-cysteinyl)amido-2-deoxy-alpha-D-glucopyranoside monobromobiane derivate Mycolicibacterium smegmatis ?
-
 ?
mycothiol bimane derivative + H2O 100% activity Mycolicibacterium smegmatis ?
-
 ?
mycothiol-3-(N-maleimidopropionyl)biocytin + H2O 5.2% activity compared to mycothiol bimane derivative Mycolicibacterium smegmatis ?
-
 ?
mycothiol-7-(diethylamino)-3-(4'-maleimidylphenyl)-4-methylcoumarin + H2O 3.8% activity compared to mycothiol bimane derivative Mycolicibacterium smegmatis ?
-
 ?
mycothiol-cerulenin + H2O 8% activity compared to mycothiol bimane derivative Mycolicibacterium smegmatis ?
-
 ?
mycothiol-N-ethylmaleimide + H2O 2.1% activity compared to mycothiol bimane derivative Mycolicibacterium smegmatis ?
-
 ?

Subunits

Subunits Comment Organism
? x * 36000, SDS-PAGE Mycolicibacterium smegmatis

Synonyms

Synonyms Comment Organism
MCA
-
 Mycolicibacterium smegmatis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8
-
 mycothiol bimane derivative at pH 7.5 and 30°C Mycolicibacterium smegmatis

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9 no significant dependence upon pH is detected from pH 7.0 to 9.0, but the activity declines sharply below pH 7.0. At pH 7.5, activity is about 25% lower in 50 mM sodium phosphate than in 25 mM HEPES Mycolicibacterium smegmatis

General Information

General Information Comment Organism
metabolism mycothiol S-conjugate amidase plays an important role in the detoxification of alkylating agents and antibiotics Mycolicibacterium smegmatis