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Literature summary for 3.5.1.114 extracted from
- Inhibition of aminoacylase 3 protects rat brain cortex neuronal cells from the toxicity of 4-hydroxy-2-nonenal mercapturate and 4-hydroxy-2-nonenal (2012), Toxicol. Appl. Pharmacol., 263, 303-314.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of the enzyme in Escherichia coli | Mus musculus |
| recombinant expression of the enzyme in Escherichia coli | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-((E)-[(4H-1,2,4-triazol-4-yl)imino]methyl)-1-benzothiophene-3-ol | - |
Rattus norvegicus |  |
| 2-(4-ethoxyphenyl)-2-methylsuccinic acid | - |
Homo sapiens | |
| 2-(4-ethoxyphenyl)-2-methylsuccinic acid | - |
Mus musculus | |
| 2-(4-ethoxyphenyl)-2-methylsuccinic acid | - |
Rattus norvegicus | |
| 2-([(3-fluoro-4-methoxyphenyl)methyl]sulfanyl)-1-methyl-1H-benzimidazole-5-sulfonamide | - |
Rattus norvegicus | |
| 2-([2-chloro-4-(thiocyanato)phenyl]carbamoyl)benzoic acid | - |
Rattus norvegicus | |
| 2-[(1,3-benzothiazol-2-yl)sulfanyl]ethan-1-amine | - |
Rattus norvegicus | |
| clioquinol | - |
Rattus norvegicus | |
| ebselen | - |
Homo sapiens | |
| ebselen | - |
Mus musculus | |
| ebselen | - |
Rattus norvegicus | |
| Ibuprofen | - |
Mus musculus | |
| additional information | the enzyme is a metalloenzyme that is inactivated by metal chelating compounds. Molecular docking, overview | Homo sapiens | |
| additional information | the enzyme is a metalloenzyme that is inactivated by metal chelating compounds. Molecular docking, overview | Mus musculus | |
| additional information | the enzyme is a metalloenzyme that is inactivated by metal chelating compounds. Molecular docking, overview | Rattus norvegicus | |
| N-(2,3,5-trichloro-4-oxocyclohexa-2,5-dien-1-ylidene)benzenesulfonamide | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Mus musculus | |
| 0.2 | - |
4-hydroxy-2-nonenal mercapturate | pH and temperature not specified in the publication, absence of Co2+ | Mus musculus | |
| 0.3 | - |
N-acetyl-L-tyrosine | pH and temperature not specified in the publication, presence of Co2+ | Mus musculus | |
| 0.45 | - |
N-acetyl-L-tyrosine | pH and temperature not specified in the publication, absence of Co2+ | Mus musculus | |
| 0.53 | - |
4-hydroxy-2-nonenal mercapturate | pH and temperature not specified in the publication, presence of Co2+ | Mus musculus | |
| 0.56 | - |
N-acetyl-S-(1,2-dichlorovinyl)-L-cysteine | pH and temperature not specified in the publication, presence of Co2+ | Mus musculus | |
| 0.71 | - |
acrolein mercapturate | pH and temperature not specified in the publication, presence of Co2+ | Mus musculus | |
| 0.82 | - |
acrolein mercapturate | pH and temperature not specified in the publication, absence of Co2+ | Mus musculus | |
| 1.3 | - |
N-acetyl-S-(1,2-dichlorovinyl)-L-cysteine | pH and temperature not specified in the publication, absence of Co2+ | Mus musculus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | metalloenzyme, highly activating | Mus musculus | |
| Co2+ | metalloenzyme, highly activating | Rattus norvegicus | |
| Co2+ | metalloenzyme, highly activating | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q96HD9 | - |
- |
| Mus musculus | Q91XE4 | - |
- |
| Rattus norvegicus | Q5M876 | - |
- |
| Rattus norvegicus Wistar | Q5M876 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli to homogeneity | Mus musculus |
| recombinant enzyme from Escherichia coli to homogeneity | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blood vessel | - |
Mus musculus | - |
| blood vessel | - |
Rattus norvegicus | - |
| blood vessel | - |
Homo sapiens | - |
| brain cortex | - |
Mus musculus | - |
| brain cortex | - |
Rattus norvegicus | - |
| brain cortex | - |
Homo sapiens | - |
| hippocampus | - |
Rattus norvegicus | - |
| neuron | - |
Rattus norvegicus | - |
| neuron | - |
Homo sapiens | - |
| neuron | mainly | Mus musculus | - |
| neuronal cell | - |
Mus musculus | - |
| neuronal cell | - |
Homo sapiens | - |
| neuronal cell | hippocampal | Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-hydroxy-2-nonenal mercapturate + H2O | substrate of aminoacylase 3, not aminoacylase 1, deacetylation | Mus musculus | ? | - |
? | |
| 4-hydroxy-2-nonenal mercapturate + H2O | substrate of aminoacylase 3, not aminoacylase 1, deacetylation | Rattus norvegicus | ? | - |
? | |
| 4-hydroxy-2-nonenal mercapturate + H2O | substrate of aminoacylase 3, not aminoacylase 1, deacetylation | Homo sapiens | ? | - |
? | |
| 4-hydroxy-2-nonenal mercapturate + H2O | substrate of aminoacylase 3, not aminoacylase 1, deacetylation | Rattus norvegicus Wistar | ? | - |
? | |
| acrolein mercapturate + H2O | substrate of aminoacylase 3, not aminoacylase 1, deacetylation | Mus musculus | ? | - |
? | |
| acrolein mercapturate + H2O | substrate of aminoacylase 3, not aminoacylase 1, deacetylation | Rattus norvegicus | ? | - |
? | |
| acrolein mercapturate + H2O | substrate of aminoacylase 3, not aminoacylase 1, deacetylation | Homo sapiens | ? | - |
? | |
| acrolein mercapturate + H2O | substrate of aminoacylase 3, not aminoacylase 1, deacetylation | Rattus norvegicus Wistar | ? | - |
? | |
| N-acetyl-L-tyrosine + H2O | - |
Mus musculus | acetate + L-tyrosine | - |
? | |
| N-acetyl-S-(1,2-dichlorovinyl)-L-cysteine + H2O | - |
Mus musculus | acetate + S-(1,2-dichlorovinyl)-L-cysteine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AA3 | - |
Mus musculus |
| AA3 | - |
Rattus norvegicus |
| AA3 | - |
Homo sapiens |
| aminoacylase 3 | - |
Mus musculus |
| aminoacylase 3 | - |
Rattus norvegicus |
| aminoacylase 3 | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
N-acetyl-S-(1,2-dichlorovinyl)-L-cysteine | pH and temperature not specified in the publication, absence of Co2+ | Mus musculus | |
| 0.27 | - |
4-hydroxy-2-nonenal mercapturate | pH and temperature not specified in the publication, absence of Co2+ | Mus musculus | |
| 0.28 | - |
N-acetyl-L-tyrosine | pH and temperature not specified in the publication, absence of Co2+ | Mus musculus | |
| 0.4 | - |
acrolein mercapturate | pH and temperature not specified in the publication, absence of Co2+ | Mus musculus | |
| 1.09 | - |
N-acetyl-S-(1,2-dichlorovinyl)-L-cysteine | pH and temperature not specified in the publication, presence of Co2+ | Mus musculus | |
| 1.67 | - |
4-hydroxy-2-nonenal mercapturate | pH and temperature not specified in the publication, presence of Co2+ | Mus musculus | |
| 2.28 | - |
N-acetyl-L-tyrosine | pH and temperature not specified in the publication, presence of Co2+ | Mus musculus | |
| 3.03 | - |
acrolein mercapturate | pH and temperature not specified in the publication, presence of Co2+ | Mus musculus | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00000063 | - |
pH and temperature not specified in the publication | Rattus norvegicus | 2-(4-ethoxyphenyl)-2-methylsuccinic acid | |
| 0.0000032 | - |
pH and temperature not specified in the publication | Rattus norvegicus | N-(2,3,5-trichloro-4-oxocyclohexa-2,5-dien-1-ylidene)benzenesulfonamide | |
| 0.00004 | - |
pH and temperature not specified in the publication | Rattus norvegicus | 2-([2-chloro-4-(thiocyanato)phenyl]carbamoyl)benzoic acid | |
| 0.000079 | - |
pH and temperature not specified in the publication | Rattus norvegicus | 2-([(3-fluoro-4-methoxyphenyl)methyl]sulfanyl)-1-methyl-1H-benzimidazole-5-sulfonamide | |
| 0.0016 | - |
pH and temperature not specified in the publication | Rattus norvegicus | 2-[(1,3-benzothiazol-2-yl)sulfanyl]ethan-1-amine | |
| 0.0101 | - |
pH and temperature not specified in the publication | Rattus norvegicus | clioquinol | |
| 0.063 | - |
pH and temperature not specified in the publication | Rattus norvegicus | 2-((E)-[(4H-1,2,4-triazol-4-yl)imino]methyl)-1-benzothiophene-3-ol | |
| 10 | - |
pH and temperature not specified in the publication | Rattus norvegicus | ebselen | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | aminoacylase 3 inhibition completely protects rat brain cortex neurons expressing the enzyme from the toxicity of 4-hydroxy-2-nonenal-mercapturate. 4-hydroxy-2-nonenal-cysteine is also neurotoxic and its toxicity is mostly prevented by a beta-lyase inhibitor, aminooxyacetate | Mus musculus |
| malfunction | aminoacylase 3 inhibition completely protects rat brain cortex neurons expressing the enzyme from the toxicity of 4-hydroxy-2-nonenal-mercapturate. 4-hydroxy-2-nonenal-cysteine is also neurotoxic and its toxicity is mostly prevented by a beta-lyase inhibitor, aminooxyacetate | Rattus norvegicus |
| malfunction | aminoacylase 3 inhibition completely protects rat brain cortex neurons expressing the enzyme from the toxicity of 4-hydroxy-2-nonenal-mercapturate. 4-hydroxy-2-nonenal-cysteine is also neurotoxic and its toxicity is mostly prevented by a beta-lyase inhibitor, aminooxyacetate | Homo sapiens |
| physiological function | the enzyme cleaves the conjugates 4-hydroxy-2-nonenal mercapturate and acrolein mercapturate formed by reaction with glutathione for detoxification in the GSH-conjugation pathway, overview. Aminoacylase 3 is involved in the neurotoxicity of the compounds 4-hydroxy-2-nonenal and acrolein | Mus musculus |
| physiological function | the enzyme cleaves the conjugates 4-hydroxy-2-nonenal mercapturate and acrolein mercapturate formed by reaction with glutathione for detoxification in the GSH-conjugation pathway, overview. Aminoacylase 3 is involved in the neurotoxicity of the compounds 4-hydroxy-2-nonenal and acrolein | Rattus norvegicus |
| physiological function | the enzyme cleaves the conjugates 4-hydroxy-2-nonenal mercapturate and acrolein mercapturate formed by reaction with glutathione for detoxification in the GSH-conjugation pathway, overview. Aminoacylase 3 is involved in the neurotoxicity of the compounds 4-hydroxy-2-nonenal and acrolein | Homo sapiens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
N-acetyl-S-(1,2-dichlorovinyl)-L-cysteine | pH and temperature not specified in the publication, absence of Co2+ | Mus musculus | |
| 0.49 | - |
acrolein mercapturate | pH and temperature not specified in the publication, absence of Co2+ | Mus musculus | |
| 0.62 | - |
N-acetyl-L-tyrosine | pH and temperature not specified in the publication, absence of Co2+ | Mus musculus | |
| 1.35 | - |
4-hydroxy-2-nonenal mercapturate | pH and temperature not specified in the publication, absence of Co2+ | Mus musculus | |
| 1.93 | - |
N-acetyl-S-(1,2-dichlorovinyl)-L-cysteine | pH and temperature not specified in the publication, presence of Co2+ | Mus musculus | |
| 3.15 | - |
4-hydroxy-2-nonenal mercapturate | pH and temperature not specified in the publication, presence of Co2+ | Mus musculus | |
| 4.27 | - |
acrolein mercapturate | pH and temperature not specified in the publication, presence of Co2+ | Mus musculus | |
| 7.6 | - |
N-acetyl-L-tyrosine | pH and temperature not specified in the publication, presence of Co2+ | Mus musculus | |
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