Application | Comment | Organism |
---|---|---|
synthesis | enzyme immobilized on alginate/chitosan hybrid beads can be used on large scale for the synthesis of beta-lactam | Escherichia coli |
General Stability | Organism |
---|---|
enzyme immobilized on alginate/chitosan hybrid beads can be reused more times as compared to enzyme immobilized on alginate beads | Escherichia coli |
enzyme immobilized on alginate/chitosan hybrid beads shows higher operational stability compared to enzyme immobilized on alginate beads | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
56.19 | - |
penicillin G | soluble enzyme, pH and temperature not specified in the publication | Escherichia coli | |
64.84 | - |
penicillin G | enzyme immobilized on alginate beads, pH and temperature not specified in the publication | Escherichia coli | |
87.08 | - |
penicillin G | enzyme immobilized on alginate/chitosan hybrid beads, pH and temperature not specified in the publication | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Storage Stability | Organism |
---|---|
enzyme immobilized on alginate/chitosan hybrid beads shows higher storage stability compared to enzyme immobilized on alginate beads | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
penicillin G + H2O | - |
Escherichia coli | 6-aminopenicillanic acid + phenylacetic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Penicillin G amidase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
soluble enzyme | Escherichia coli |
50 | - |
enzyme immobilized on alginate/chitosan hybrid beads and enzyme immobilized on alginate beads | Escherichia coli |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | 30°C: about 85% of maximal activity, 70°C: about 85% of maximal activity, enzyme immobilized on alginate/chitosan hybrid beads | Escherichia coli |
30 | 70 | 30°C: about 95% of maximal activity, 70°C: about 65% of maximal activity, soluble enzyme | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
thermal stability of the enzyme immobilized on alginate/chitosan hybrid beads is much higher that that of the soluble enzyme | Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
10 | - |
stability of the enzyme immobilized on alginate/chitosan hybrid beads is higher that that of the enzyme immobilized an alginate. | Escherichia coli |