Any feedback?
Literature summary for 3.5.1.11 extracted from
- Penicillin V acylase from Pectobacterium atrosepticum exhibits high specific activity and unique kinetics (2015), Int. J. Biol. Macromol., 79, 1-7 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-butanone | 5% (v/v), 30 min at room temperature, 1.74fold activation | Pectobacterium atrosepticum |  |
| 2-mercaptoethanol | 1 mM, 1.19fold activation | Pectobacterium atrosepticum | |
| acetonitrile | 5% (v/v), 30 min at room temperature, 1.65fold activation | Pectobacterium atrosepticum | |
| CTAB | 0.1%, 2.5fold activation | Pectobacterium atrosepticum | |
| dithiothreitol | 10 mM, 1.23fold activation | Pectobacterium atrosepticum | |
| ethyl acetate | 5% (v/v), 30 min at room temperature, 1.47fold activation | Pectobacterium atrosepticum | |
| hexane | 5% (v/v), 30 min at room temperature, 1.13fold activation | Pectobacterium atrosepticum | |
| Isopropanol | 5% (v/v), 30 min at room temperature, 1.95fold activation | Pectobacterium atrosepticum | |
| Triton X-100 | 0.1%, 1.8fold activation | Pectobacterium atrosepticum | |
| Tween 20 | 0.1%, 1.75fold activation | Pectobacterium atrosepticum | |
Application
| Application | Comment | Organism |
|---|---|---|
| pharmacology | both the recombinant protein yield and the specific activity of the enzyme are very high, auguring well for application in the pharmaceutical industry for production of the pharmaceutical intermediates 6-amino penicillanic acid. Penicillin acylases find use in the pharmaceutical industry for the production of semi-synthetic antibiotics | Pectobacterium atrosepticum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli, attaining a very high yield (250 mg/l) and a comparatively high specific activity (430 IU/mg) | Pectobacterium atrosepticum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| SDS | 0.1%, 90% loss of activity | Pectobacterium atrosepticum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 154000 | - |
gel filtration | Pectobacterium atrosepticum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pectobacterium atrosepticum | Q6D291 | - |
- |
| Pectobacterium atrosepticum ATCC BAA-672 | Q6D291 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pectobacterium atrosepticum |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| the enzyme starts progressively losing activity after 1.5 M guanidine hydrochloride, and is completelly inactivated at concentrations over 2 M. Dilution of guanidine hydrochloride leads to revival of the original enzyme activity within 1 h, indicating that unfolding is completely reversible | Pectobacterium atrosepticum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-nitro-5-(phenoxyacetamido)-benzoic acid + H2O | - |
Pectobacterium atrosepticum | 5-amino-2-nitrobenzoic acid + phenoxyacetate | - |
? | |
| 2-nitro-5-(phenoxyacetamido)-benzoic acid + H2O | - |
Pectobacterium atrosepticum ATCC BAA-672 | 5-amino-2-nitrobenzoic acid + phenoxyacetate | - |
? | |
| penicillin V + H2O | - |
Pectobacterium atrosepticum | 6-aminopenicillanic acid + phenoxyacetic acid | - |
? | |
| penicillin V + H2O | - |
Pectobacterium atrosepticum ATCC BAA-672 | 6-aminopenicillanic acid + phenoxyacetic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 39191, matrix-associated laser desorption ionization-mass spectrometry | Pectobacterium atrosepticum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Eca3205 | - |
Pectobacterium atrosepticum |
| penicillin V acylase | - |
Pectobacterium atrosepticum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Pectobacterium atrosepticum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 60 | 35°C: about 60% of maximal activity, 60°C: about 35% of maximal activity | Pectobacterium atrosepticum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
15 min, the enzyme retains 87% of its original activity | Pectobacterium atrosepticum |
| 40 | - |
90 min, about 35% loss of activity | Pectobacterium atrosepticum |
| 50 | - |
90 min, about 50% loss of activity | Pectobacterium atrosepticum |
| 60 | - |
60 min, about 80% loss of activity | Pectobacterium atrosepticum |
| 70 | - |
30 min, about 80% loss of activity | Pectobacterium atrosepticum |
| 80 | - |
15 min, about 90% loss of activity | Pectobacterium atrosepticum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
acetate buffer | Pectobacterium atrosepticum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6 | pH 4.0: about 40% of maximal activity, pH 6.0: about 35% of maximal activity | Pectobacterium atrosepticum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | the enzyme is functionally stable in the pH range 3-7 with than 80% retention in activity after 4 h. Significant reduction in activity at pH 9-11, with only 40% of the original activity retained. The enzyme is rapidly inactivated at pH 1-2 | Pectobacterium atrosepticum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pectobacterium atrosepticum | - |
- |
8.4 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
