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Literature summary for 3.5.1.11 extracted from

  • Avinash, V.; Pundle, A.; Ramasamy, S.; Suresh, C.
    Penicillin acylases revisited Importance beyond their industrial utility (2016), Crit. Rev. Biotechnol., 36, 303-316 .
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0197
-
6-nitro-3-(phenylacetamido)-benzoic acid pH and temperature not specified in the publication Providencia rettgeri
0.42
-
penicillin G pH and temperature not specified in the publication Rhizobium viscosum
1.83
-
penicillin G pH and temperature not specified in the publication Priestia megaterium

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Lysinibacillus sphaericus 5737
-
cytoplasm
-
Achromobacter sp. 5737
-
extracellular
-
Rhizobium viscosum
-
-
intracellular
-
Bacillus badius 5622
-
periplasm
-
Achromobacter xylosoxidans
-
-
periplasm
-
Escherichia coli
-
-

Organism

Organism UniProt Comment Textmining
Achromobacter sp.
-
-
-
Achromobacter xylosoxidans
-
-
-
Alcaligenes faecalis
-
-
-
Bacillus badius
-
-
-
Bacillus subtilis
-
-
-
Escherichia coli P06875
-
-
Fusarium oxysporum
-
-
-
Kluyvera cryocrescens P07941
-
-
Lysinibacillus sphaericus
-
-
-
Priestia megaterium Q60136
-
-
Providencia rettgeri
-
-
-
Rhizobium viscosum P31956
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-nitro-3-(phenylacetamido)-benzoic acid + H2O
-
Alcaligenes faecalis 3-amino-6-nitrobenzoic acid + phenylacetate
-
?
6-nitro-3-(phenylacetamido)-benzoic acid + H2O
-
Achromobacter sp. 3-amino-6-nitrobenzoic acid + phenylacetate
-
?
6-nitro-3-(phenylacetamido)-benzoic acid + H2O
-
Achromobacter xylosoxidans 3-amino-6-nitrobenzoic acid + phenylacetate
-
?
6-nitro-3-(phenylacetamido)-benzoic acid + H2O
-
Providencia rettgeri 3-amino-6-nitrobenzoic acid + phenylacetate
-
?
6-nitro-3-(phenylacetamido)-benzoic acid + H2O
-
Bacillus badius 3-amino-6-nitrobenzoic acid + phenylacetate
-
?
6-nitro-3-(phenylacetamido)-benzoic acid + H2O
-
Escherichia coli 3-amino-6-nitrobenzoic acid + phenylacetate
-
?
6-nitro-3-(phenylacetamido)-benzoic acid + H2O
-
Kluyvera cryocrescens 3-amino-6-nitrobenzoic acid + phenylacetate
-
?
ampicillin + H2O
-
Bacillus badius 6-aminopenicillanic acid + (R)-2-phenylglycine
-
?
ampicillin + H2O
-
Achromobacter sp. (R)-2-phenylglycine + 6-aminopenicillanic acid
-
?
cephalexin + H2O
-
Achromobacter sp. 7-aminodesacetoxycephalosporanic acid + D-phenylglycine
-
?
cephalexin + H2O
-
Bacillus badius 7-aminodesacetoxycephalosporanic acid + D-phenylglycine
-
?
cephalothin + H2O
-
Bacillus badius (6R,7R)-3-[(acetyloxy)methyl]-7-amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid + (thiophen-2-yl)acetic acid
-
?
penicillin G + H2O
-
Alcaligenes faecalis 6-aminopenicillanic acid + phenylacetic acid
-
?
penicillin G + H2O
-
Achromobacter sp. 6-aminopenicillanic acid + phenylacetic acid
-
?
penicillin G + H2O
-
Achromobacter xylosoxidans 6-aminopenicillanic acid + phenylacetic acid
-
?
penicillin G + H2O
-
Providencia rettgeri 6-aminopenicillanic acid + phenylacetic acid
-
?
penicillin G + H2O
-
Bacillus badius 6-aminopenicillanic acid + phenylacetic acid
-
?
penicillin G + H2O
-
Priestia megaterium 6-aminopenicillanic acid + phenylacetic acid
-
?
penicillin G + H2O
-
Escherichia coli 6-aminopenicillanic acid + phenylacetic acid
-
?
penicillin G + H2O
-
Kluyvera cryocrescens 6-aminopenicillanic acid + phenylacetic acid
-
?
penicillin G + H2O
-
Rhizobium viscosum 6-aminopenicillanic acid + phenylacetic acid
-
?
penicillin V + H2O
-
Lysinibacillus sphaericus phenoxyacetate + 6-aminopenicillinate
-
?
penicillin V + H2O
-
Fusarium oxysporum phenoxyacetate + 6-aminopenicillinate
-
?
penicillin V + H2O specific for penicillin V Bacillus subtilis phenoxyacetate + 6-aminopenicillinate
-
?

Synonyms

Synonyms Comment Organism
penicillin G acylase
-
Alcaligenes faecalis
penicillin G acylase
-
Achromobacter sp.
penicillin G acylase
-
Achromobacter xylosoxidans
penicillin G acylase
-
Providencia rettgeri
penicillin G acylase
-
Bacillus badius
penicillin G acylase
-
Priestia megaterium
penicillin G acylase
-
Escherichia coli
penicillin G acylase
-
Kluyvera cryocrescens
penicillin G acylase
-
Rhizobium viscosum
penicillin V acylase
-
Bacillus subtilis
penicillin V acylase
-
Lysinibacillus sphaericus
penicillin V acylase
-
Fusarium oxysporum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
thermostable enzyme Alcaligenes faecalis
additional information
-
thermostable enzyme Achromobacter xylosoxidans

General Information

General Information Comment Organism
physiological function possible scavenging of phenylacetyl compounds, cell signalling Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3
-
Penicillin V pH and temperature not specified in the publication Bacillus subtilis
4.1
-
Penicillin V pH and temperature not specified in the publication Lysinibacillus sphaericus
5.2
-
Penicillin V pH and temperature not specified in the publication Fusarium oxysporum
21
-
ampicillin pH and temperature not specified in the publication Bacillus badius
58.2
-
cephalexin pH and temperature not specified in the publication Bacillus badius
86
-
cephalothin pH and temperature not specified in the publication Bacillus badius
805
-
6-nitro-3-(phenylacetamido)-benzoic acid pH and temperature not specified in the publication Bacillus badius
1025
-
penicillin G pH and temperature not specified in the publication Bacillus badius
1700
-
6-nitro-3-(phenylacetamido)-benzoic acid pH and temperature not specified in the publication Escherichia coli
2500
-
6-nitro-3-(phenylacetamido)-benzoic acid pH and temperature not specified in the publication Kluyvera cryocrescens
2600
-
6-nitro-3-(phenylacetamido)-benzoic acid pH and temperature not specified in the publication Achromobacter xylosoxidans
3100
-
penicillin G pH and temperature not specified in the publication Kluyvera cryocrescens
4200
-
6-nitro-3-(phenylacetamido)-benzoic acid pH and temperature not specified in the publication Achromobacter sp.
8200
-
penicillin G pH and temperature not specified in the publication Achromobacter xylosoxidans
10000
-
penicillin G pH and temperature not specified in the publication Escherichia coli
13000
-
penicillin G pH and temperature not specified in the publication Alcaligenes faecalis
15000
-
penicillin G pH and temperature not specified in the publication Achromobacter sp.
21000
-
6-nitro-3-(phenylacetamido)-benzoic acid pH and temperature not specified in the publication Alcaligenes faecalis