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Literature summary for 3.5.1.11 extracted from

  • Torres-Bacete, J.; Hormigo, D.; Torres-Guzman, R.; Arroyo, M.; Castillon, M.; Garcia, J.; Acebal, C.; de la Mata, I.
    Overexpression of penicillin V acylase from Streptomyces lavendulae and elucidation of its catalytic residues (2015), Appl. Environ. Microbiol., 81, 1225-1233 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.11

Cloned(Commentary)

Cloned (Comment) Organism
the heterologous expression of pva in Streptomyces lividans leads to the production of an extracellularly homogeneous heterodimeric enzyme at a 5fold higher concentration than in the original host and in a considerably shorter time Streptomyces lavendulae subsp. lavendulae

Protein Variants

Protein Variants Comment Organism
N272D mutation at beta-subunit results in a drastic decrease of activity Streptomyces lavendulae subsp. lavendulae
N272V mutation at beta-subunit results in a drastic decrease of activity Streptomyces lavendulae subsp. lavendulae
S1A mutation at beta-subunit results in an inactive enzyme Streptomyces lavendulae subsp. lavendulae
S1C mutation at beta-subunit results in an inactive enzyme Streptomyces lavendulae subsp. lavendulae
S1D mutation at beta-subunit results in an inactive enzyme Streptomyces lavendulae subsp. lavendulae
S1H mutation at beta-subunit results in an inactive enzyme Streptomyces lavendulae subsp. lavendulae
S1K mutation at beta-subunit results in an inactive enzyme Streptomyces lavendulae subsp. lavendulae
V70A mutation at beta-subunit results in a drastic decrease of activity Streptomyces lavendulae subsp. lavendulae
V70D mutation at beta-subunit results in a drastic decrease of activity Streptomyces lavendulae subsp. lavendulae

Inhibitors

Inhibitors Comment Organism Structure
Na+ activity is slightly enhanced at 0.6 M NaCl, reaches 100% activity at 1 M NaCl, and is gradually inhibited by higher NaCl concentrations, retaining 60% activity at 3 M NaCl Streptomyces lavendulae subsp. lavendulae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
 penicillin K pH 8.0, 40°C recombinant enzyme Streptomyces lavendulae subsp. lavendulae
0.82
-
 penicillin dihydroF pH 8.0, 40°C recombinant enzyme Streptomyces lavendulae subsp. lavendulae
1.39
-
 penicillin F pH 8.0, 40°C recombinant enzyme Streptomyces lavendulae subsp. lavendulae
3.68
-
 Penicillin V pH 8.0, 40°C recombinant enzyme Streptomyces lavendulae subsp. lavendulae
58.18
-
 penicillin G pH 8.0, 40°C recombinant enzyme Streptomyces lavendulae subsp. lavendulae

Metals/Ions

Metals/Ions Comment Organism Structure
Na+ activity is slightly enhanced at 0.6 M NaCl, reaches 100% activity at 1 M NaCl, and is gradually inhibited by higher NaCl concentrations, retaining 60% activity at 3 M NaCl Streptomyces lavendulae subsp. lavendulae

Organism

Organism UniProt Comment Textmining
Streptomyces lavendulae subsp. lavendulae Q539C0
-
-
Streptomyces lavendulae subsp. lavendulae ATCC 13664 Q539C0
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the gene encodes an inactive precursor protein containing a secretion signal peptide that is activated by two internal autoproteolytic cleavages that release a 25-amino-acid linker peptide and two large domains of 18.79 kDa (alpha-subunit) and 60.09 kDa (beta-subunit) Streptomyces lavendulae subsp. lavendulae

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces lavendulae subsp. lavendulae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aculeacin A + H2O recombinant enzyme Streptomyces lavendulae subsp. lavendulae ?
-
 ?
penicillin dihydroF + H2O
-
 Streptomyces lavendulae subsp. lavendulae 6-aminopenicillanic acid + hexanoic acid
-
 ?
penicillin dihydroF + H2O
-
 Streptomyces lavendulae subsp. lavendulae ATCC 13664 6-aminopenicillanic acid + hexanoic acid
-
 ?
penicillin F + H2O
-
 Streptomyces lavendulae subsp. lavendulae 6-aminopenicillanic acid + (3E)-hex-3-enoic acid
-
 ?
penicillin F + H2O
-
 Streptomyces lavendulae subsp. lavendulae ATCC 13664 6-aminopenicillanic acid + (3E)-hex-3-enoic acid
-
 ?
penicillin G + H2O
-
 Streptomyces lavendulae subsp. lavendulae phenylacetic acid + 6-aminopenicillanic acid
-
 ?
penicillin G + H2O
-
 Streptomyces lavendulae subsp. lavendulae ATCC 13664 phenylacetic acid + 6-aminopenicillanic acid
-
 ?
penicillin K + H2O
-
 Streptomyces lavendulae subsp. lavendulae octanoic acid + 6-aminopenicillanate
-
 ?
penicillin K + H2O
-
 Streptomyces lavendulae subsp. lavendulae ATCC 13664 octanoic acid + 6-aminopenicillanate
-
 ?
penicillin V + H2O
-
 Streptomyces lavendulae subsp. lavendulae phenoxyacetate + 6-aminopenicillinate
-
 ?
penicillin V + H2O
-
 Streptomyces lavendulae subsp. lavendulae ATCC 13664 phenoxyacetate + 6-aminopenicillinate
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 18790 (alpha-subunit) + 1 * 60090 (beta-subunit), MALDI-TOF Streptomyces lavendulae subsp. lavendulae
heterodimer 1 * 21400 (alpha-subunit) + 1 * 59000 (beta-subunit), SDS-PAGE, the beta-subunit loses a fragment of 11000 Da when the enzyme is stored at 4°C , although the enzyme remains active, indicating that the 11000 Da fragment remains attached to the enzyme via noncovalent bonds and that the structure of the enzyme is not affect by the proteolytic effect Streptomyces lavendulae subsp. lavendulae

Synonyms

Synonyms Comment Organism
penicillin V acylase
-
 Streptomyces lavendulae subsp. lavendulae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 substrate: penicillin V, recombinant enzyme Streptomyces lavendulae subsp. lavendulae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 stable up to 45°C, recombinant enzyme Streptomyces lavendulae subsp. lavendulae
62
-
 Tm-value of recombinant enzyme Streptomyces lavendulae subsp. lavendulae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.33
-
 penicillin F pH 8.0, 40°C recombinant enzyme Streptomyces lavendulae subsp. lavendulae
10.95
-
 penicillin G pH 8.0, 40°C recombinant enzyme Streptomyces lavendulae subsp. lavendulae
14.75
-
 penicillin dihydroF pH 8.0, 40°C recombinant enzyme Streptomyces lavendulae subsp. lavendulae
35.58
-
 penicillin K pH 8.0, 40°C recombinant enzyme Streptomyces lavendulae subsp. lavendulae
77.92
-
 Penicillin V pH 8.0, 40°C recombinant enzyme Streptomyces lavendulae subsp. lavendulae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5 10 substrate: penicillin V, recombinant enzyme Streptomyces lavendulae subsp. lavendulae

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 10.5 stable between pH 7.0 and 10.5, recombinant enzyme Streptomyces lavendulae subsp. lavendulae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.18
-
 penicillin G pH 8.0, 40°C recombinant enzyme Streptomyces lavendulae subsp. lavendulae
2.4
-
 penicillin F pH 8.0, 40°C recombinant enzyme Streptomyces lavendulae subsp. lavendulae
17.93
-
 penicillin dihydroF pH 8.0, 40°C recombinant enzyme Streptomyces lavendulae subsp. lavendulae
21.47
-
 Penicillin V pH 8.0, 40°C recombinant enzyme Streptomyces lavendulae subsp. lavendulae
244.11
-
 penicillin K pH 8.0, 40°C recombinant enzyme Streptomyces lavendulae subsp. lavendulae