Protein Variants | Comment | Organism |
---|---|---|
alphaF146Y | bad catalyst for synthesis | Escherichia coli |
betaF24A | reduced hydrolytic activity towards amides of phenylglycine | Escherichia coli |
betaF24A/alphaF146Y | very low amidase activity | Escherichia coli |
betaF24T | used as negative control because of the negative effect on synthetic and hydrolytic activities | Escherichia coli |
betaF24T/alphaF146Y | used as negative control because of the negative effect on synthetic and hydrolytic activities | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P06875 | - |
- |
Purification (Comment) | Organism |
---|---|
ion exchange chromatography (Q-Sepharose) | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.7 | - |
substrate penicillin G, betaF24A/alphaF146Y mutant protein, pH 8.0, 37°C | Escherichia coli |
3 | 8 | substrate penicillin G, wild type protein, pH 8.0, 37°C | Escherichia coli |
11 | - |
substrate penicillin G, betaF24A mutant protein, pH 8.0, 37°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-nitro-3-(phenylacetamido)benzoic acid + H2O | - |
Escherichia coli | ? | - |
? | |
7-amino-3-propenyl-cephalosporanic acid + 4-hydroxy-D-phenylglycine methyl ester | - |
Escherichia coli | cefprozil + ? | - |
? | |
D-phenylglycine amide + 7-aminodeacetoxycephalosporanic acid | - |
Escherichia coli | cephalexin + ? | - |
? | |
D-phenylglycine amide + 7-aminodesacetoxymethyl-3-chlorocephalosporanic acid | - |
Escherichia coli | cephaclor + ? | - |
? | |
D-phenylglycine methyl ester + 7-aminodeacetoxycephalosporanic acid | - |
Escherichia coli | cephalexin + ? | - |
? | |
D-phenylglycine methyl ester + 7-aminodesacetoxymethyl-3-chlorocephalosporanic acid | - |
Escherichia coli | cephaclor + ? | - |
? | |
penicillin G + H2O | - |
Escherichia coli | phenyl acetic acid + 6-aminopenicillanate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
penicillin acylase | - |
Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
10 | - |
stable: 2 h at 20°C or 4°C, mutant betaF24A and mutant betaF24A/alphaF146Y: more than 80% activity after 2 h at 4°C, less than 10% activity after 2 h at 20°C | Escherichia coli |