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Literature summary for 3.5.1.11 extracted from

  • Cecchini, D.A.; Pavesi, R.; Sanna, S.; Daly, S.; Xaiz, R.; Pregnolato, M.; Terreni, M.
    Efficient biocatalyst for large-scale synthesis of cephalosporins, obtained by combining immobilization and site-directed mutagenesis of penicillin acylase (2012), Appl. Microbiol. Biotechnol., 95, 1491-1500.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
alphaF146Y bad catalyst for synthesis Escherichia coli
betaF24A reduced hydrolytic activity towards amides of phenylglycine Escherichia coli
betaF24A/alphaF146Y very low amidase activity Escherichia coli
betaF24T used as negative control because of the negative effect on synthetic and hydrolytic activities Escherichia coli
betaF24T/alphaF146Y used as negative control because of the negative effect on synthetic and hydrolytic activities Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P06875
-
-

Purification (Commentary)

Purification (Comment) Organism
ion exchange chromatography (Q-Sepharose) Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.7
-
substrate penicillin G, betaF24A/alphaF146Y mutant protein, pH 8.0, 37°C Escherichia coli
3 8 substrate penicillin G, wild type protein, pH 8.0, 37°C Escherichia coli
11
-
substrate penicillin G, betaF24A mutant protein, pH 8.0, 37°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-nitro-3-(phenylacetamido)benzoic acid + H2O
-
Escherichia coli ?
-
?
7-amino-3-propenyl-cephalosporanic acid + 4-hydroxy-D-phenylglycine methyl ester
-
Escherichia coli cefprozil + ?
-
?
D-phenylglycine amide + 7-aminodeacetoxycephalosporanic acid
-
Escherichia coli cephalexin + ?
-
?
D-phenylglycine amide + 7-aminodesacetoxymethyl-3-chlorocephalosporanic acid
-
Escherichia coli cephaclor + ?
-
?
D-phenylglycine methyl ester + 7-aminodeacetoxycephalosporanic acid
-
Escherichia coli cephalexin + ?
-
?
D-phenylglycine methyl ester + 7-aminodesacetoxymethyl-3-chlorocephalosporanic acid
-
Escherichia coli cephaclor + ?
-
?
penicillin G + H2O
-
Escherichia coli phenyl acetic acid + 6-aminopenicillanate
-
?

Synonyms

Synonyms Comment Organism
penicillin acylase
-
Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
10
-
stable: 2 h at 20°C or 4°C, mutant betaF24A and mutant betaF24A/alphaF146Y: more than 80% activity after 2 h at 4°C, less than 10% activity after 2 h at 20°C Escherichia coli