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Literature summary for 3.5.1.11 extracted from

  • Pinotti, L.; Fonseca, L.; Prazeres, D.; Rodrigues, D.; Nucci, E.; Giordano, R.
    Recovery and partial purification of penicillin G acylase from E. coli homogenate and B. megaterium culture medium using an expanded bed adsorption column (2009), Biochem. Eng. J., 44, 111-118.
No PubMed abstract available
Search for more literature for 3.5.1.11

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Priestia megaterium
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20500
-
 1 * 20500 + 1 * 69000, SDS-PAGE Escherichia coli
26900
-
 1 * 26900 + 1 * 59100, SDS-PAGE Priestia megaterium
59100
-
 1 * 26900 + 1 * 59100, SDS-PAGE Priestia megaterium
69000
-
 1 * 20500 + 1 * 69000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
penicillin G + H2O Escherichia coli
-
 phenylacetic acid + 6-aminopenicillanate
-
 ?
penicillin G + H2O Priestia megaterium
-
 phenylacetic acid + 6-aminopenicillanate
-
 ?
penicillin G + H2O Priestia megaterium ATCC 14945
-
 phenylacetic acid + 6-aminopenicillanate
-
 ?
penicillin G + H2O Escherichia coli ATCC 9637
-
 phenylacetic acid + 6-aminopenicillanate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli ATCC 9637
-
-
-
Priestia megaterium
-
-
-
Priestia megaterium ATCC 14945
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 12.7fold from fermentation broth by expanded bed adsorption method using a cationic resin, method comparisons show similar results for packed-bed and for expanded-bed modes, evaluation, overview Priestia megaterium
native enzyme 4.5-7.5fold from crude cell extract or homogenate by expanded bed adsorption method using a cationic resin, method comparisons show similar results for packed-bed and for expanded-bed modes, evaluation, overview Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
penicillin G + H2O
-
 Escherichia coli phenylacetic acid + 6-aminopenicillanate
-
 ?
penicillin G + H2O
-
 Priestia megaterium phenylacetic acid + 6-aminopenicillanate
-
 ?
penicillin G + H2O
-
 Escherichia coli phenylacetic acid + 6-aminopenicillanate product detection by p-dimethylamine benzaldehyde ?
penicillin G + H2O
-
 Priestia megaterium phenylacetic acid + 6-aminopenicillanate product detection by p-dimethylamine benzaldehyde ?
penicillin G + H2O
-
 Priestia megaterium ATCC 14945 phenylacetic acid + 6-aminopenicillanate
-
 ?
penicillin G + H2O
-
 Priestia megaterium ATCC 14945 phenylacetic acid + 6-aminopenicillanate product detection by p-dimethylamine benzaldehyde ?
penicillin G + H2O
-
 Escherichia coli ATCC 9637 phenylacetic acid + 6-aminopenicillanate
-
 ?
penicillin G + H2O
-
 Escherichia coli ATCC 9637 phenylacetic acid + 6-aminopenicillanate product detection by p-dimethylamine benzaldehyde ?

Subunits

Subunits Comment Organism
heterodimer 1 * 20500 + 1 * 69000, SDS-PAGE Escherichia coli
heterodimer 1 * 26900 + 1 * 59100, SDS-PAGE Priestia megaterium

Synonyms

Synonyms Comment Organism
penicillin G acylase
-
 Escherichia coli
penicillin G acylase
-
 Priestia megaterium
PGA
-
 Escherichia coli
PGA
-
 Priestia megaterium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli
37
-
 assay at Priestia megaterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Escherichia coli
8
-
 assay at Priestia megaterium

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 4.8 high inactivation at pH 4.0, but stable at pH 4.8 Priestia megaterium
4.5 4.8 high inactivation at pH 4.5, but stable at pH 4.8 Escherichia coli