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Literature summary for 3.5.1.11 extracted from
- Production of enantiomerically pure (S)-beta-phenylalanine and (R)-beta-phenylalanine by penicillin G acylase from Escherichia coli in aqueous medium (2007), Biotechnol. Lett., 29, 1825-1830.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | penicillin G acylase-catalyzed acylation of beta-phenylalanine with phenylacetamide as an acyl donor is efficient with a high preferential enantioselectivity for (R)-beta-phenylalanine. Both (S)-beta-phenylalanine, at 98% ee, and (R)-beta-phenylalanine, at 99% ee, can be produced using simple separation procedures | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-beta-phenylalanine + phenylacetamide | the acylation reaction is highly preferential for the acylation of (R)-beta-phenylalanine | Escherichia coli | N-phenylacetyl-(R)-beta-phenylalanine + ? | - |
? |  |
| penicillin G + H2O | - |
Escherichia coli | 6-aminopenicillanate + phenylacetic acid | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| penicillin G acylase | - |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
acylation of beta-phenylalanine | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 11 | pH 8: about 50% of maximal activity, pH 11: about 60% of maximal activity | Escherichia coli |
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