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Literature summary for 3.5.1.11 extracted from

  • Yang, Y.; Biedendieck, R.; Wang, W.; Gamer, M.; Malten, M.; Jahn, D.; Deckwer, W.D.
    High yield recombinant penicillin G amidase production and export into the growth medium using Bacillus megaterium (2006), Microb. Cell Fact., 5, 36.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.1.11

Application

Application Comment Organism
synthesis production of 6-aminopenicillanate as precursor for the production of semi-synthetic beta-lactam derivatives Priestia megaterium

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Bacillus megaterium MS941, strain is deficient in the major extracellular protease NprM due to deletion of the corresponding gene, expressed in the newly constructed Bacillus megaterium YYBm1 strain, which has a xylose utilization deficiency, 7fold higher enzyme expression compared with host strain Priestia megaterium

Protein Variants

Protein Variants Comment Organism
additional information leader peptide was replaced by the Bacillus megaterium LipA counterpart, changing the signal peptide increases the amount of secreted enzyme Priestia megaterium

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Priestia megaterium
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information 2.5 mM CaCl2 in the medium is optimal for enzyme production Priestia megaterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 1 * 27000 + 1 * 57000, alpha, beta subunits produced by autoproteolytic cleavage, SDS-PAGE Priestia megaterium
27753
-
 1 * 27753 + 1 * 61394, alpha,beta, calculated from the deduced amino acid sequence Priestia megaterium
57000
-
 1 * 27000 + 1 * 57000, alpha, beta subunits produced by autoproteolytic cleavage, SDS-PAGE Priestia megaterium
61394
-
 1 * 27753 + 1 * 61394, alpha,beta, calculated from the deduced amino acid sequence Priestia megaterium
90000
-
-
 Priestia megaterium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Priestia megaterium autoproteolytic processing of the alpha and beta subunit ?
-
 ?
penicillin G + H2O Priestia megaterium function in nature not fully understood, possibly functions to degrade phenylacetylated compounds in order to generate phenylacetic acid which can be used as carbon source 6-aminopenicillanic acid + phenylacetic acid
-
 ?

Organism

Organism UniProt Comment Textmining
Priestia megaterium
-
 strain ATCC 14945, grown on buffered mineral medium containing calcium ions and defined amino acid supplements for optimal PGA production
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification autoproteolytic cleavage which results in the formation of two subunits, the alpha and beta subunits range from residue 25 to 265 and from 266 to 802 with calculated molecular masses of 27753 Da and 61394 Da, respectively Priestia megaterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-nitro-6-phenylacetaminobenzoic acid + H2O
-
 Priestia megaterium 6-nitrophenol + ?
-
 ?
additional information autoproteolytic processing of the alpha and beta subunit Priestia megaterium ?
-
 ?
penicillin G + H2O function in nature not fully understood, possibly functions to degrade phenylacetylated compounds in order to generate phenylacetic acid which can be used as carbon source Priestia megaterium 6-aminopenicillanic acid + phenylacetic acid
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 27000 + 1 * 57000, alpha, beta subunits produced by autoproteolytic cleavage, SDS-PAGE Priestia megaterium
dimer 1 * 27753 + 1 * 61394, alpha,beta, calculated from the deduced amino acid sequence Priestia megaterium

Synonyms

Synonyms Comment Organism
penicillin G acylase
-
 Priestia megaterium
PGA
-
 Priestia megaterium