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Literature summary for 3.5.1.11 extracted from
- High expression of the penicillin G acylase gene (pac) from Bacillus megaterium UN1 in its own pac minus mutant (2000), J. Appl. Microbiol., 89, 152-157.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | production of 6-aminopenicillanate as precursor for the production of semi-synthetic beta-lactam derivatives | Priestia megaterium |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in enzyme deficient Bacillus megaterium strain UN-cat, 20fold higher expression rate than in host strain | Priestia megaterium |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Priestia megaterium | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
1 * 27000 + 1 * 57000, alpha, beta, SDS-PAGE, the alpha and beta subunits range from residue 25 to 265 and from 266 to 802 | Priestia megaterium |
| 57000 | - |
1 * 27000 + 1 * 57000, alpha, beta, SDS-PAGE, the alpha and beta subunits range from residue 25 to 265 and from 266 to 802 | Priestia megaterium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| penicillin G + H2O | Priestia megaterium | - |
6-aminopenicillanic acid + phenylacetic acid | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Priestia megaterium | - |
strain UN1 and enzyme deficient strain UN-cat | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Bacillus megaterium UN-cat | Priestia megaterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| penicillin G + H2O | - |
Priestia megaterium | 6-aminopenicillanic acid + phenylacetic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 27000 + 1 * 57000, alpha, beta, SDS-PAGE, the alpha and beta subunits range from residue 25 to 265 and from 266 to 802 | Priestia megaterium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PAC | - |
Priestia megaterium |
| penicillin G acylase | - |
Priestia megaterium |
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