Cloned (Comment) | Organism |
---|---|
- |
Alcaligenes faecalis |
- |
Escherichia coli |
- |
Providencia rettgeri |
- |
Kluyvera cryocrescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alcaligenes faecalis | - |
- |
- |
Escherichia coli | - |
- |
- |
Kluyvera cryocrescens | - |
- |
- |
Providencia rettgeri | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-tagged recombinant enzyme | Alcaligenes faecalis |
His-tagged recombinant enzyme | Escherichia coli |
His-tagged recombinant enzyme | Providencia rettgeri |
His-tagged recombinant enzyme | Kluyvera cryocrescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1975 | - |
30°C, pH 7.0 | Providencia rettgeri |
12840 | - |
30°C, pH 7.0, high intrinsic synthseis/hydrolysis ratio | Kluyvera cryocrescens |
17990 | - |
30°C, pH 7.0 | Alcaligenes faecalis |
22240 | - |
30°C, pH 7.0 | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-phenylglycine amide + 7-aminodesacetoxycephalosporanic acid | kinetically controlled synthesis | Alcaligenes faecalis | cephalexin + NH3 | - |
r | |
D-phenylglycine amide + 7-aminodesacetoxycephalosporanic acid | kinetically controlled synthesis | Escherichia coli | cephalexin + NH3 | - |
r | |
D-phenylglycine amide + 7-aminodesacetoxycephalosporanic acid | kinetically controlled synthesis | Providencia rettgeri | cephalexin + NH3 | - |
r | |
D-phenylglycine amide + 7-aminodesacetoxycephalosporanic acid | kinetically controlled synthesis | Kluyvera cryocrescens | cephalexin + NH3 | - |
r |