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Literature summary for 3.5.1.11 extracted from

  • Kumar, R.S.; Suresh, C.G.; Pundle, A.; Prabhune, A.
    Evidence for the involvement of arginyl residue at the active site of penicillin G acylase from Kluyvera citrophila (2004), Biotechnol. Lett., 26, 1601-1606.
    View publication on PubMed
Search for more literature for 3.5.1.11

Inhibitors

Inhibitors Comment Organism Structure
2,3-Butanedione 50 mM, 78% loss of activity, benzylpenicillin or phenylacetate protects Kluyvera cryocrescens
Phenylglyoxal 20 mM, 82% loss of activity, benzylpenicillin or phenylacetate protects Kluyvera cryocrescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 benzylpenicillin pH 7.5, 40°C Kluyvera cryocrescens
0.061
-
 benzylpenicillin pH 7.5, 40°C, presence of 50 mM 2,3-butanedione Kluyvera cryocrescens
0.065
-
 benzylpenicillin pH 7.5, 40°C, presence of 10 mM phenylglyoxal Kluyvera cryocrescens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 x * 24000, alpha-subunit, + x * 62000, beta-subunit, SDS-PAGE Kluyvera cryocrescens
62000
-
 x * 24000, alpha-subunit, + x * 62000, beta-subunit, SDS-PAGE Kluyvera cryocrescens

Organism

Organism UniProt Comment Textmining
Kluyvera cryocrescens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Kluyvera cryocrescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18
-
 pH 7.5, 40°C Kluyvera cryocrescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzylpenicillin + H2O
-
 Kluyvera cryocrescens phenylacetic acid + 6-aminopenicillanate
-
 ?
penicillin G + H2O
-
 Kluyvera cryocrescens 6-aminopenicillanic acid + phenylacetic acid
-
 ?

Subunits

Subunits Comment Organism
? x * 24000, alpha-subunit, + x * 62000, beta-subunit, SDS-PAGE Kluyvera cryocrescens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
21
-
 benzylpenicillin pH 7.5, 40°C, presence of 10 mM phenylglyoxal Kluyvera cryocrescens
27
-
 benzylpenicillin pH 7.5, 40°C, presence of 50 mM 2,3-butanedione Kluyvera cryocrescens
63
-
 benzylpenicillin pH 7.5, 40°C Kluyvera cryocrescens