Literature summary for 3.5.1.11 extracted from

Scaramozzino, F.; Estruch, I.; Rossolillo, P.; Terreni, M.; Albertini, A.M.
Improvement of catalytic properties of Escherichia coli penicillin G acylase immobilized on glyoxyl agarose by addition of a six-amino-acid tag (2005), Appl. Environ. Microbiol., 71, 8937-8940.

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Protein Variants

Protein Variants	Comment	Organism
additional information	addition of a tag of three lysine residues alternating with three glycines to C-terminus of enzyme results in improvement of the immobilization efficiency on glyoxyl agarose and the catalytic protperties of immobilized enzyme, but impairs posttranslational steps of overexpressed protein maturation	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
12	-	substrate (R)-mandelic acid methyl ester, mutant with three lysine residues alternating with three glycines at C-terminus, pH 8.5, 25°C	Escherichia coli
12	-	substrate (R)-mandelic acid methyl ester, wild-type, pH 8.5, 25°C	Escherichia coli
12	-	substrate 6-nitro-3-[(phenylacetyl)amino]benzoic acid, mutant with three lysine residues alternating with three glycines at C-terminus, pH 8.5, 25°C	Escherichia coli
16	-	substrate 6-nitro-3-[(phenylacetyl)amino]benzoic acid, wild-type, pH 8.5, 25°C	Escherichia coli
18	-	substrate penicillin G, mutant with three lysine residues alternating with three glycines at C-terminus, pH 8.5, 25°C	Escherichia coli
33	-	substrate penicillin G, wild-type, pH 8.5, 25°C	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(R)-mandelic acid methyl ester + H2O	-	Escherichia coli	?	-	?
6-nitro-3-[(phenylacetyl)amino]benzoic acid + H2O	-	Escherichia coli	3-amino-6-nitrobenzoic acid + phenylacetic acid	-	?
penicillin G + H2O	-	Escherichia coli	6-aminopenicillanate + phenylacetic acid	-	?

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Release 2023.1 (January 2023)