Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | decreases enzyme activity to 50% | Pseudomonas melanogenum | |
diethyldicarbonate | - |
Pseudomonas melanogenum | |
Fe2+ | decreases enzyme activity to 50% | Pseudomonas melanogenum | |
Hg2+ | decreases enzyme activity to 50% | Pseudomonas melanogenum | |
N-bromosuccinimide | - |
Pseudomonas melanogenum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.61 | - |
cephalexin | - |
Pseudomonas melanogenum | |
1.79 | - |
cephadrine | - |
Pseudomonas melanogenum | |
1.96 | - |
cephaloglycin | - |
Pseudomonas melanogenum | |
4.34 | - |
ampicillin | - |
Pseudomonas melanogenum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Pseudomonas melanogenum | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
2 * 72000, SDS-PAGE | Pseudomonas melanogenum |
146000 | - |
gel filtration | Pseudomonas melanogenum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas melanogenum | other natural penicillins | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas melanogenum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas melanogenum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ampicillin + H2O | - |
Pseudomonas melanogenum | 6-aminopenicillanic acid + (R)-2-phenylglycine | - |
r | |
cephadrine + H2O | - |
Pseudomonas melanogenum | ? | - |
? | |
cephalexin + H2O | - |
Pseudomonas melanogenum | 7-amino-3-methyl-8-oxo-5-thia-1-aza-bicyclo[4.2.0]oct-2-ene-2-carboxylic acid + D-phenylglycine | - |
? | |
cephaloglycin + H2O | - |
Pseudomonas melanogenum | (6R,7R)-7-amino-3-(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid + (2R)-amino(phenyl)acetic acid | - |
? | |
additional information | other natural penicillins | Pseudomonas melanogenum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 72000, SDS-PAGE | Pseudomonas melanogenum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Pseudomonas melanogenum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Pseudomonas melanogenum |